Load time: 01/31/2012 11:23:06 AM Read time: 01/31/2012 11:23:06 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2180 Comment: Wheat Plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 94661016 cps Min counts: 856 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 78447376.00 94661016.00 85408856.00 42156.00 2992930.00 2016.00 9302.00 1921.00 1997.00 1940.00 1807.00 2092.00 B 64621708.00 62167572.00 53204928.00 52105.00 4223361.00 1921.00 1959.00 2092.00 2092.00 2016.00 1959.00 1959.00 C 35242384.00 30717036.00 29929944.00 131281.00 52276.00 2054.00 2016.00 2092.00 2054.00 1940.00 2054.00 1864.00 D 19442966.00 18077762.00 20077716.00 49746.00 59581.00 1940.00 2149.00 1997.00 2130.00 1902.00 2092.00 1883.00 E 9676331.00 9197479.00 8841679.00 51287.00 50906.00 1959.00 2130.00 2035.00 2035.00 2054.00 1864.00 2035.00 F 6852870.00 6029893.00 5523694.00 48871.00 47863.00 2016.00 2149.00 1712.00 2016.00 1921.00 2035.00 1864.00 G 3185662.00 3268251.00 3260732.00 49841.00 51515.00 1940.00 2149.00 2358.00 2701.00 2168.00 2016.00 1902.00 H 2424339.00 1852332.00 2311156.00 46721.00 59981.00 2130.00 1902.00 2035.00 1940.00 2092.00 1978.00 2073.00