Load time: 05/04/2012 01:42:44 PM Read time: 05/04/2012 01:42:44 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2542 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56618892 cps Min counts: 1085 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56618892.00 53705004.00 53037076.00 29462.00 2436.00 31606282.00 32154114.00 32955040.00 25477.00 2436.00 2417.00 2285.00 B 47060248.00 50297920.00 41409288.00 29613.00 2492.00 27436316.00 29557492.00 26771374.00 24457.00 2322.00 2398.00 2360.00 C 18329884.00 24444074.00 29687490.00 30500.00 2417.00 18786154.00 17868462.00 17644910.00 25420.00 2436.00 2549.00 2417.00 D 16370850.00 16645691.00 17565258.00 32257.00 2832.00 12185034.00 11520413.00 10682855.00 25817.00 2322.00 2417.00 12502.00 E 9644222.00 10118215.00 10011621.00 31822.00 2795.00 7495294.00 7305817.00 7008000.00 25231.00 2379.00 2322.00 2492.00 F 3612391.00 3839127.00 4004328.00 31539.00 2757.00 5130587.00 4518239.00 5406152.00 25590.00 2474.00 2398.00 2266.00 G 2320891.00 2441308.00 2632654.00 30784.00 2606.00 3470964.00 2912502.00 2486507.00 33446.00 2171.00 2606.00 2360.00 H 1732330.00 1787077.00 1803795.00 29556.00 2209.00 2080001.00 1667793.00 1656735.00 26138.00 2285.00 2360.00 2322.00