Load time: 10/04/2011 11:32:59 AM Read time: 10/04/2011 11:32:59 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1913 Comment: standards final Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42280952 cps Min counts: 13145 counts Well list: r0(a1:h8) = cps(f2(a1:h8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40299140.00 42079620.00 42280952.00 46712.00 8920556.00 10414797.00 12131844.00 26290.00 B 18372018.00 18553652.00 18996200.00 47912.00 17667856.00 8243461.00 9741680.00 29947.00 C 10639212.00 10768430.00 11520074.00 50332.00 5975385.00 12531299.00 8251926.00 32957.00 D 5910102.00 6107623.00 5852996.00 50865.00 6165079.00 10611044.00 10198185.00 33434.00 E 3890983.00 3650684.00 3504271.00 50503.00 10297411.00 6463875.00 11614813.00 33662.00 F 2350057.00 2280350.00 2205922.00 50808.00 6914337.00 9655314.00 43283.00 34310.00 G 1536034.00 1489243.00 1418140.00 50846.00 13250967.00 18313638.00 44712.00 35339.00 H 944823.00 943566.00 897787.00 47760.00 10090613.00 7958399.00 49646.00 33777.00