Load time: 07/15/2013 10:57:28 AM Read time: 07/15/2013 10:57:28 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3131 Comment: run 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 28.9 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 142240288 cps Min counts: 1133 counts Well list: r0(a1:h5,a6:a8,b6:f8,g6:g8,a9:e9) = cps(f2(a1:h5,a6:a8,b6:f8,g6:g8,a9:e9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2493.00 110494344.00 128103616.00 142240288.00 49550.00 1842903.00 147800.00 1010061.00 903102.00 B 2417.00 81605488.00 83878096.00 83658632.00 50947.00 5795303.00 3672115.00 2328274.00 235471.00 C 2304.00 46948528.00 49123484.00 48352964.00 52326.00 5390811.00 1266784.00 388050.00 268945.00 D 2455.00 30978972.00 31833012.00 32090858.00 54499.00 9007280.00 1485008.00 6477830.00 1707937.00 E 2266.00 16414789.00 17078618.00 16542884.00 51892.00 7726491.00 2668798.00 2182827.00 438016.00 F 2304.00 8669848.00 9179132.00 8737754.00 52138.00 6122923.00 2601683.00 7117876.00 G 2342.00 4869800.00 4855578.00 4802620.00 49115.00 3218857.00 6892725.00 4064172.00 H 2417.00 3083608.00 3057329.00 3085226.00 44902.00 Load time: 07/15/2013 10:59:19 AM Read time: 07/15/2013 10:59:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3132 Comment: run 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 29 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 133861352 cps Min counts: 1133 counts Well list: r0(a1:h5,a6:a8,b6:f8,g6:g8,a9:e9) = cps(f2(a1:h5,a6:a8,b6:f8,g6:g8,a9:e9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2436.00 102112664.00 124868264.00 133861352.00 37724.00 1683490.00 118292.00 941280.00 863375.00 B 2266.00 78664392.00 82570368.00 81817752.00 43599.00 5644976.00 3523448.00 2274556.00 224004.00 C 2417.00 44622252.00 47684588.00 46943404.00 45186.00 5266215.00 1235615.00 365193.00 258234.00 D 2663.00 29177412.00 31308520.00 30993616.00 48681.00 8739255.00 1444903.00 6427319.00 1686351.00 E 2417.00 15644036.00 16316073.00 15980576.00 45526.00 7536710.00 2607108.00 2137450.00 416858.00 F 2399.00 8350966.00 8796701.00 8481184.00 44657.00 5900272.00 2567495.00 6779584.00 G 2493.00 4669749.00 4618393.00 4673819.00 41974.00 3137570.00 6782294.00 3861025.00 H 2342.00 2967958.00 2979311.00 2956109.00 38725.00