Load time: 06/03/2011 02:43:43 PM Read time: 06/03/2011 02:43:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1585 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 71205712 cps Min counts: 942 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 70775128.00 66537268.00 71205712.00 39927.00 35968.00 2112.00 2055.00 1884.00 2207.00 2017.00 2055.00 2017.00 B 60424892.00 42411632.00 50579584.00 38557.00 36273.00 2188.00 2074.00 2074.00 2112.00 2017.00 2131.00 2074.00 C 30831314.00 25589242.00 27417674.00 38081.00 34941.00 2169.00 2169.00 2493.00 2036.00 1941.00 2169.00 2150.00 D 19252284.00 18634250.00 15848036.00 39394.00 35226.00 2036.00 2055.00 2207.00 2093.00 2150.00 1941.00 2359.00 E 9770640.00 9616737.00 9504965.00 37624.00 34769.00 1979.00 1960.00 2093.00 2169.00 2188.00 2074.00 3025.00 F 5773758.00 5982799.00 6052491.00 97591.00 32828.00 2226.00 2055.00 2207.00 2188.00 2245.00 2036.00 2074.00 G 3219942.00 2898914.00 3155587.00 38481.00 33380.00 2150.00 2283.00 2188.00 2378.00 2131.00 2055.00 2169.00 H 1871265.00 1822169.00 1891088.00 40174.00 35093.00 2302.00 2283.00 2150.00 2207.00 2017.00 2093.00 2283.00 Load time: 06/03/2011 02:47:24 PM Read time: 06/03/2011 02:47:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1586 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 4827564 cps Min counts: 18622 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1395138.00 997101.00 747963.00 2590690.00 552856.00 4827564.00 4073975.00 1230195.00 1885103.00 2792066.00 39946.00 1705269.00 B 1873414.00 1033812.00 683352.00 2716355.00 1335551.00 1303027.00 4023097.00 974720.00 957972.00 4422396.00 41982.00 1767941.00 C 747716.00 1298041.00 1125276.00 3360704.00 1237978.00 2901214.00 2607212.00 1959575.00 1791136.00 4804900.00 41050.00 613108.00 D 737972.00 1280190.00 1655075.00 2634822.00 1549329.00 2869050.00 4544307.00 2295924.00 2723842.00 4243816.00 38214.00 861656.00 E 1036038.00 1439214.00 1430308.00 2191401.00 1223401.00 2112345.00 3917511.00 1559917.00 2418617.00 4537634.00 37738.00 969296.00 F 760486.00 1678736.00 976718.00 1776789.00 1315226.00 3131462.00 2632787.00 2381214.00 2186265.00 3278336.00 38233.00 763112.00 G 724422.00 596894.00 568670.00 1110793.00 2164878.00 1284852.00 1750771.00 1627939.00 2376070.00 3678032.00 37244.00 856213.00 H 1350890.00 1446008.00 1109042.00 2352228.00 1855683.00 877375.00 2809606.00 1976418.00 1699137.00 4045962.00 37795.00 2094978.00