Load time: 06/09/2011 01:56:56 PM Read time: 06/09/2011 01:56:56 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1598 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10334210 cps Min counts: 33733 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3893501.00 3067007.00 5685835.00 3723895.00 4497452.00 4259766.00 2172722.00 5542825.00 5400217.00 4986651.00 67466.00 71619.00 B 5126488.00 3232467.00 3268889.00 5437420.00 4483632.00 5240718.00 7977541.00 4554264.00 4381874.00 4713257.00 3727081.00 93123.00 C 3420687.00 4126220.00 7689524.00 2027889.00 3945787.00 8491936.00 5457763.00 4879381.00 6290385.00 5676068.00 3167499.00 71771.00 D 4939337.00 5391183.00 5517187.00 4405967.00 4174504.00 5143691.00 7394871.00 5743996.00 7529113.00 6891713.00 73714.00 76952.00 E 6127564.00 5205878.00 4825833.00 6709581.00 9563023.00 7514514.00 4690065.00 6222521.00 10334210.00 6952946.00 8076074.00 104038.00 F 4704974.00 6570283.00 6968857.00 6885090.00 4918390.00 6830273.00 5906429.00 7325178.00 6484973.00 7209996.00 7617711.00 71657.00 G 4362099.00 2986024.00 4890354.00 5851578.00 4770106.00 7415092.00 6037661.00 6665699.00 5986497.00 6962129.00 74914.00 71028.00 H 6263554.00 4888007.00 5275625.00 1077773.00 5271140.00 7515558.00 8898008.00 7733007.00 8353560.00 7504591.00 73695.00 72133.00 Load time: 06/09/2011 01:59:13 PM Read time: 06/09/2011 01:59:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1599 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10775933 cps Min counts: 34333 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4160679.00 3269375.00 5940591.00 3980668.00 4720103.00 4451730.00 2271784.00 5740957.00 5587091.00 5080622.00 68666.00 72723.00 B 5388677.00 3338771.00 3397230.00 5607346.00 4559779.00 5342519.00 8072890.00 4664788.00 4593133.00 4943609.00 3899973.00 96685.00 C 3651260.00 4302169.00 7928905.00 2121141.00 4142509.00 8857640.00 5660329.00 5144794.00 6481498.00 5947742.00 3269033.00 73485.00 D 5378731.00 5490332.00 5657329.00 4735948.00 4492224.00 5211672.00 7855561.00 6147848.00 7966047.00 7221795.00 76438.00 79162.00 E 6570681.00 5373960.00 5136160.00 7097624.00 10213575.00 7862693.00 4864282.00 6685125.00 10775933.00 7301417.00 8489579.00 105943.00 F 5174902.00 6946465.00 7175375.00 7201703.00 5076698.00 6995307.00 6013240.00 7538082.00 6654920.00 7433829.00 7937705.00 72933.00 G 4700597.00 3207842.00 5100778.00 6083920.00 5037787.00 7637576.00 6190537.00 6888269.00 6247149.00 7175045.00 76152.00 72571.00 H 6670088.00 5220796.00 5549489.00 1138401.00 5463755.00 7809186.00 9142005.00 7949028.00 8501346.00 7620159.00 74038.00 74762.00 Load time: 06/09/2011 01:59:13 PM Read time: 06/09/2011 01:59:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1599 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10775933 cps Min counts: 34333 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4160679.00 3269375.00 5940591.00 3980668.00 4720103.00 4451730.00 2271784.00 5740957.00 5587091.00 5080622.00 68666.00 72723.00 B 5388677.00 3338771.00 3397230.00 5607346.00 4559779.00 5342519.00 8072890.00 4664788.00 4593133.00 4943609.00 3899973.00 96685.00 C 3651260.00 4302169.00 7928905.00 2121141.00 4142509.00 8857640.00 5660329.00 5144794.00 6481498.00 5947742.00 3269033.00 73485.00 D 5378731.00 5490332.00 5657329.00 4735948.00 4492224.00 5211672.00 7855561.00 6147848.00 7966047.00 7221795.00 76438.00 79162.00 E 6570681.00 5373960.00 5136160.00 7097624.00 10213575.00 7862693.00 4864282.00 6685125.00 10775933.00 7301417.00 8489579.00 105943.00 F 5174902.00 6946465.00 7175375.00 7201703.00 5076698.00 6995307.00 6013240.00 7538082.00 6654920.00 7433829.00 7937705.00 72933.00 G 4700597.00 3207842.00 5100778.00 6083920.00 5037787.00 7637576.00 6190537.00 6888269.00 6247149.00 7175045.00 76152.00 72571.00 H 6670088.00 5220796.00 5549489.00 1138401.00 5463755.00 7809186.00 9142005.00 7949028.00 8501346.00 7620159.00 74038.00 74762.00