##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 TimeFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 -27.75 4969693.75 6567894.75 4258717.75 -5282.25 34128.25 428238.25 374281.25 -17.75 -7.75 -71.75 34.25 20.25 4401768.75 4231410.75 3635116.75 44081.75 79424.25 878112.25 102292.25 164.25 26.25 -8.75 -44.75 22.25 2479112.75 2446972.75 1117178.75 -6515.25 21779.25 392447.25 461537.25 4.25 26.25 -10.75 -22.75 -4.75 1476707.75 1321505.75 1336758.75 -5267.25 14513.25 481898.25 1319888.25 116.25 -13.75 38.25 4.25 -6.75 915703.75 826321.75 589968.75 -6318.25 41191.25 591787.25 7890.25 50.25 80.25 -57.75 1.25 -39.75 472874.75 475592.75 418432.75 -7018.25 9143.25 710334.25 8009.25 69.25 52.25 -18.75 50.25 43.25 294685.75 300947.75 285603.75 -6662.25 340714.25 94975.25 7257.25 29.25 -26.75 54.25 -56.75 -6.75 176160.75 182576.75 73314.75 -7018.25 848356.25 590572.25 7643.25 62.25 31.25 -12.75 -12.75 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 0.100 0.088 A2 4969693.750 5265435.417 1182654.622 22.5 0.117 A3 6567894.750 0.075 A4 4258717.750 02 0.060 0.078 B2 4401768.750 4089432.083 402563.385 9.8 0.075 B3 4231410.750 0.064 B4 3635116.750 03 0.036 0.043 C2 2479112.750 2014421.417 777201.098 38.6 0.042 C3 2446972.750 0.018 C4 1117178.750 04 0.022 0.025 D2 1476707.750 1378324.083 85543.398 6.2 0.022 D3 1321505.750 0.022 D4 1336758.750 05 0.013 0.015 E2 915703.750 777331.417 168302.888 21.7 0.013 E3 826321.750 0.009 E4 589968.750 06 0.008 0.007 F2 472874.750 455633.417 32245.373 7.1 0.007 F3 475592.750 0.006 F4 418432.750 07 0.005 0.003 G2 294685.750 293745.750 7715.069 2.6 0.004 G3 300947.750 0.003 G4 285603.750 08 0.003 0.001 H2 176160.750 144017.417 61314.285 42.6 0.001 H3 182576.750 -0.001 H4 73314.750 Group Summaries MinStd Smallest standard value: 144017.417 Min(MeanValue) MaxStd Largest standard value: 5265435.417 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A6 34128.250 R -0.001 0.001 0.005 736.8 A7 428238.250 0.006 A8 374281.250 0.005 A9 -17.750 R -0.002 A10 -7.750 R -0.002 A11 -71.750 R -0.002 A12 34.250 R -0.002 B6 79424.250 R -0.000 B7 878112.250 0.014 B8 102292.250 R 0.000 B9 164.250 R -0.002 B10 26.250 R -0.002 B11 -8.750 R -0.002 B12 -44.750 R -0.002 C6 21779.250 R -0.001 C7 392447.250 0.005 C8 461537.250 0.007 C9 4.250 R -0.002 C10 26.250 R -0.002 C11 -10.750 R -0.002 C12 -22.750 R -0.002 D6 14513.250 R -0.002 D7 481898.250 0.007 D8 1319888.250 0.022 D9 116.250 R -0.002 D10 -13.750 R -0.002 D11 38.250 R -0.002 D12 4.250 R -0.002 E6 41191.250 R -0.001 E7 591787.250 0.009 E8 7890.250 R -0.002 E9 50.250 R -0.002 E10 80.250 R -0.002 E11 -57.750 R -0.002 E12 1.250 R -0.002 F6 9143.250 R -0.002 F7 710334.250 0.011 F8 8009.250 R -0.002 F9 69.250 R -0.002 F10 52.250 R -0.002 F11 -18.750 R -0.002 F12 50.250 R -0.002 G6 340714.250 0.004 G7 94975.250 R -0.000 G8 7257.250 R -0.002 G9 29.250 R -0.002 G10 -26.750 R -0.002 G11 54.250 R -0.002 G12 -56.750 R -0.002 H6 848356.250 0.013 H7 590572.250 0.009 H8 7643.250 R -0.002 H9 62.250 R -0.002 H10 31.250 R -0.002 H11 -12.750 R -0.002 H12 -12.750 R -0.002 Group Summaries InRange R - Outside standard range ~End Original Filename: 2019_07_10_Interspecies_RNA-seq_library; Date Last Saved: 7/10/2019 4:03:23 PM