Load time: 02/08/2012 02:51:30 PM Read time: 02/08/2012 02:51:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2198 Comment: Standard and Nagu Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16324576 cps Min counts: 12998 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1108183.00 1568811.00 2794711.00 2513573.00 4552781.00 7871553.00 10742325.00 795240.00 1183792.00 2692437.00 3253605.00 11756589.00 B 2419235.00 287441.00 3184183.00 1976095.00 1364489.00 703549.00 9343642.00 2434591.00 3603278.00 4236441.00 1962830.00 12903139.00 C 2368314.00 1069797.00 2357292.00 2295538.00 2803044.00 1355849.00 8521250.00 1830891.00 2369424.00 1517573.00 5281760.00 16324576.00 D 3807350.00 1790642.00 2582499.00 444540.00 4375946.00 3594372.00 8843554.00 1117907.00 2454077.00 2555849.00 9013988.00 6563413.00 E 2503475.00 1044277.00 2572559.00 1014037.00 1025931.00 2031146.00 6054411.00 4928171.00 1593124.00 3374900.00 29745.00 6740599.00 F 2019405.00 1812852.00 698582.00 1236907.00 2512657.00 2254748.00 5655732.00 2701940.00 2065882.00 2359221.00 27118.00 9297104.00 G 2942238.00 513508.00 2855317.00 1811808.00 753277.00 3492515.00 4040684.00 3090201.00 2980046.00 1481643.00 25996.00 26528.00 H 1184496.00 1422058.00 951312.00 1701702.00 1130068.00 2722395.00 3758158.00 1069265.00 2473492.00 2562515.00 26034.00 28032.00