Load time: 02/08/2012 02:48:47 PM Read time: 02/08/2012 02:48:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2197 Comment: Standard and Nagu Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 109830752 cps Min counts: 15100 counts Well list: r0(a2:h5) = cps(f2(a2:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 92529056.00 92458808.00 109830752.00 33665.00 B 75549224.00 77080712.00 76724888.00 31419.00 C 43251352.00 47096108.00 50617652.00 30201.00 D 30391356.00 29221748.00 29456512.00 31667.00 E 16926594.00 17415960.00 17413352.00 32675.00 F 9770140.00 9354956.00 11119251.00 31476.00 G 6395912.00 6398794.00 6691243.00 31438.00 H 3562049.00 3796138.00 4086248.00 32447.00