Load time: 12/21/2011 09:51:34 AM Read time: 12/21/2011 09:51:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2089 Comment: p1_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 103781424 cps Min counts: 24241 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103781424.00 96780632.00 98807976.00 15616828.00 12648224.00 24551610.00 15345350.00 19425364.00 25492998.00 21011598.00 22349630.00 21207694.00 B 64091372.00 84128800.00 79263400.00 20273954.00 21591240.00 24356036.00 6093387.00 24056362.00 25792142.00 24493696.00 24599460.00 22115240.00 C 37090640.00 41111736.00 40945512.00 22383036.00 25284910.00 24086202.00 18789086.00 26049150.00 26148512.00 20701988.00 22106890.00 20461136.00 D 25495168.00 24466530.00 26060832.00 22489430.00 24130232.00 30451876.00 17819094.00 24804748.00 25871916.00 41571288.00 21883930.00 18679658.00 E 16783116.00 16677731.00 15546401.00 11724884.00 21319516.00 25553310.00 22921210.00 25150254.00 31995390.00 25038260.00 22388376.00 15783441.00 F 11092159.00 10832998.00 10529963.00 18774592.00 26781680.00 22259810.00 17217208.00 15120282.00 19933168.00 19436610.00 23973568.00 21318582.00 G 6171314.00 4965419.00 5310246.00 22343860.00 25063806.00 27839424.00 24817714.00 25064290.00 23095848.00 24213966.00 24209824.00 18135506.00 H 4056026.00 3709058.00 3604834.00 50592.00 49679.00 48482.00 49622.00 50497.00 434931.00 50763.00 49413.00 265168.00