Load time: 07/03/2012 08:39:49 AM Read time: 07/03/2012 08:39:49 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2730 Comment: samples 1-90 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 122127848 cps Min counts: 27612 counts Well list: r0(a2:h7) = cps(f2(a2:h7)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 102721504.00 122127848.00 120410568.00 59098.00 8219741.00 8874678.00 B 74693120.00 78737104.00 75978992.00 56641.00 9115621.00 7733385.00 C 45853916.00 50699772.00 50812920.00 59003.00 7653214.00 5054966.00 D 27199406.00 27690442.00 29017040.00 57095.00 6120437.00 10832407.00 E 15779455.00 17485652.00 16246394.00 56584.00 197158.00 4083981.00 F 8319857.00 10430825.00 8890343.00 57851.00 8464624.00 1592518.00 G 5702462.00 5610269.00 6306215.00 56603.00 12295633.00 6386076.00 H 3455452.00 3530119.00 3288032.00 55224.00 1140446.00 3904033.00