Load time: 01/27/2012 12:00:27 PM Read time: 01/27/2012 12:00:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2167 Comment: Wheat Plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 80243088 cps Min counts: 18882 counts Well list: r0(a1:h9) = cps(f2(a1:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56509984.00 30041406.00 47607988.00 37764.00 17655758.00 14578919.00 25981696.00 13794052.00 11521069.00 B 80243088.00 35037428.00 70980720.00 46625.00 32862944.00 42551980.00 26793888.00 31612866.00 7606560.00 C 30934482.00 46570968.00 40619112.00 51075.00 24732598.00 21255032.00 24568838.00 11948576.00 3280630.00 D 32528334.00 23895060.00 64590000.00 52368.00 37063000.00 12978812.00 19671702.00 2768047.00 10640480.00 E 10368128.00 11960575.00 23557764.00 53984.00 28632982.00 27070372.00 17909168.00 20549994.00 12258067.00 F 11754475.00 14261389.00 5488234.00 51721.00 20914470.00 48241380.00 23750860.00 20370588.00 9665192.00 G 5497818.00 7260073.00 4489788.00 50695.00 40437120.00 36115712.00 29405360.00 50390.00 11261145.00 H 3189450.00 2398174.00 3259397.00 68854.00 15590152.00 31797814.00 24870484.00 46302.00 42195.00