Load time: 08/29/2013 10:19:35 AM Read time: 08/29/2013 10:19:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3194 Comment: Standards; Blanks Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 48450936 cps Min counts: 1369 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48450936.00 47410096.00 45441296.00 3476.00 3684.00 3627.00 3703.00 3760.00 3495.00 3741.00 3476.00 3268.00 B 28353554.00 30219502.00 30133026.00 3590.00 3552.00 3514.00 3401.00 3646.00 3741.00 3514.00 3854.00 3457.00 C 17236366.00 18287432.00 17385274.00 3495.00 3741.00 3533.00 4308.00 3495.00 3609.00 3722.00 4289.00 3533.00 D 10425423.00 10675711.00 11486061.00 2739.00 3401.00 3533.00 3760.00 3571.00 3911.00 3665.00 4119.00 3476.00 E 6355700.00 6099509.00 6585508.00 2796.00 3987.00 3741.00 3287.00 3590.00 3457.00 3495.00 4062.00 3590.00 F 3828347.00 3690411.00 4299089.00 3401.00 3495.00 3798.00 3495.00 3646.00 3609.00 3741.00 4346.00 3609.00 G 2397788.00 2320776.00 2437202.00 3816.00 3495.00 3741.00 3287.00 3495.00 3722.00 3533.00 4119.00 3514.00 H 1428764.00 1381808.00 1514607.00 3665.00 3193.00 3722.00 3533.00 3646.00 3571.00 3722.00 3816.00 3363.00 Load time: 08/29/2013 10:23:11 AM Read time: 08/29/2013 10:23:11 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3195 Comment: DNA from Plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29165024 cps Min counts: 8002 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2403744.00 3327153.00 2895225.00 2963024.00 4041103.00 967027.00 249140.00 248819.00 119307.00 289652.00 3076496.00 66872.00 B 2819231.00 743755.00 2981437.00 7930164.00 3464806.00 3339416.00 413968.00 128944.00 4345144.00 1382961.00 4708982.00 30233.00 C 2746586.00 1894932.00 5745494.00 3711476.00 4493504.00 4602415.00 112902.00 824704.00 2017821.00 2759571.00 4351435.00 32236.00 D 1248933.00 4253944.00 4909751.00 7208328.00 3412138.00 2639728.00 3693014.00 2256322.00 1762232.00 244189.00 29165024.00 18309.00 E 2615162.00 2939317.00 5956075.00 610559.00 6570291.00 6230361.00 385926.00 1794872.00 2060521.00 1400269.00 2667155.00 16004.00 F 1485602.00 4566306.00 3654705.00 7454568.00 8429960.00 4653915.00 2073445.00 954367.00 1549645.00 1435037.00 3224672.00 51509.00 G 2400080.00 3485432.00 5332103.00 3946862.00 5074047.00 5611058.00 2285350.00 1016969.00 306545.00 197611.00 6716617.00 22278.00 H 2434177.00 2759805.00 3236188.00 2582619.00 3494542.00 3807049.00 348078.00 156381.00 732304.00 67212.00 1253147.00 41343.00