Load time: 08/30/2013 09:50:07 AM Read time: 08/30/2013 09:50:07 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3197 Comment: Standards; Blanks Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29763678 cps Min counts: 1096 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27293526.00 27321658.00 29763678.00 19729.00 19786.00 2192.00 2456.00 2381.00 2211.00 2532.00 2494.00 2475.00 B 25862626.00 25901746.00 26847746.00 19729.00 19219.00 2456.00 2494.00 2456.00 2362.00 2362.00 2343.00 2589.00 C 17177586.00 18023272.00 17938858.00 19143.00 20598.00 2305.00 2966.00 2437.00 2456.00 2437.00 2381.00 2248.00 D 10051606.00 10881644.00 10820055.00 18538.00 30406.00 2437.00 2305.00 2551.00 2607.00 2532.00 2532.00 2532.00 E 5848162.00 5782702.00 6614456.00 18123.00 19597.00 2494.00 2626.00 2456.00 2267.00 2381.00 2305.00 2456.00 F 3526741.00 3450091.00 3323210.00 20296.00 19653.00 2551.00 2456.00 2324.00 2513.00 2418.00 2437.00 2381.00 G 2358292.00 2445793.00 2450056.00 20069.00 19805.00 2343.00 2400.00 2437.00 2475.00 2494.00 2229.00 2343.00 H 1267614.00 1301668.00 1368964.00 20844.00 20466.00 2437.00 2362.00 2418.00 2381.00 2513.00 2324.00 2248.00 Load time: 08/30/2013 09:53:23 AM Read time: 08/30/2013 09:53:23 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3198 Comment: Plate 8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9571741 cps Min counts: 12085 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1355546.00 8874950.00 3739232.00 2979389.00 1086232.00 3793049.00 3707858.00 3224920.00 5187465.00 4142183.00 1429928.00 1848105.00 B 4166402.00 8908883.00 5281971.00 5995765.00 903395.00 4413486.00 2805049.00 3249675.00 4175894.00 9571741.00 3465497.00 3273655.00 C 4393469.00 5248566.00 5485548.00 7247490.00 7601466.00 3451280.00 4014901.00 3735936.00 5738405.00 6367352.00 3672519.00 383892.00 D 231518.00 4985056.00 5129966.00 6545574.00 5497099.00 4570494.00 3367966.00 156833.00 6306850.00 5223264.00 73267.00 5192235.00 E 3051615.00 50684.00 5054535.00 5061608.00 5874271.00 5854155.00 2992827.00 2962969.00 132190.00 3777573.00 3132581.00 3528592.00 F 861574.00 9137396.00 3302357.00 127712.00 3763475.00 5074869.00 1191078.00 75043.00 4486656.00 4731892.00 3407757.00 3107830.00 G 1554776.00 4596211.00 373876.00 4119383.00 1363257.00 3761842.00 3509704.00 3344435.00 6395878.00 3822795.00 2956261.00 3286812.00 H 3425684.00 2934224.00 24170.00 2666462.00 2762429.00 2483955.00 3334849.00 2822416.00 2981072.00 3914235.00 1126693.00 458028.00 Load time: 08/30/2013 09:56:20 AM Read time: 08/30/2013 09:56:20 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3199 Comment: Plate 9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14817061 cps Min counts: 10516 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3446414.00 8012700.00 6941384.00 5342030.00 3853608.00 6299198.00 3886555.00 3694236.00 3751784.00 231178.00 1830237.00 5239479.00 B 21033.00 4882313.00 7970604.00 9958690.00 3380512.00 8029940.00 4091090.00 4541528.00 12854990.00 4448604.00 3642463.00 3837297.00 C 3630053.00 4377312.00 7647148.00 5388987.00 4584661.00 6136563.00 7560829.00 5187045.00 7793843.00 36132.00 3341498.00 3939194.00 D 3983913.00 7621645.00 5942275.00 10156110.00 9159162.00 14817061.00 10505204.00 5304253.00 5872026.00 4759047.00 4660129.00 4018133.00 E 3314355.00 7313777.00 6956931.00 7285972.00 6228866.00 3385907.00 5663287.00 2764321.00 608588.00 4617223.00 4346743.00 2955411.00 F 2887187.00 5732183.00 523490.00 3474269.00 5997373.00 2621502.00 4783539.00 4042773.00 5901515.00 2525490.00 5027712.00 2770605.00 G 1589310.00 4933430.00 4304940.00 5938961.00 4528233.00 4573565.00 5873737.00 3684033.00 8650453.00 2137861.00 4103028.00 3325958.00 H 1641194.00 4048226.00 4115581.00 3345482.00 3428796.00 4356465.00 3873815.00 2579616.00 1797007.00 2274604.00 4390398.00 1991955.00 Load time: 08/30/2013 09:59:03 AM Read time: 08/30/2013 09:59:03 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3200 Comment: Plate 10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19327388 cps Min counts: 13634 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3870956.00 1937920.00 11516135.00 3560968.00 5803108.00 12463178.00 48473.00 3561101.00 3476793.00 8204973.00 27269.00 3555774.00 B 3528424.00 2517602.00 4433845.00 5734655.00 12970207.00 19327388.00 4199819.00 4633238.00 3683170.00 4492692.00 12367179.00 4300502.00 C 4691431.00 3809569.00 5912040.00 9760434.00 6004565.00 10878141.00 7966264.00 15835172.00 7387679.00 6320533.00 10470234.00 5761868.00 D 4929797.00 4814638.00 4302113.00 12579560.00 13657551.00 9694181.00 7599205.00 6730138.00 8284703.00 5640293.00 6149355.00 4316841.00 E 5936779.00 4854136.00 13595829.00 6598381.00 5140134.00 12398637.00 6457921.00 11586637.00 3889494.00 5804313.00 5158643.00 6018712.00 F 2996049.00 4141939.00 11594708.00 6360953.00 4788695.00 4951095.00 10909064.00 7134538.00 2415645.00 4936084.00 5274391.00 4397607.00 G 4931022.00 5631581.00 4310733.00 8540351.00 6112865.00 3549904.00 7634921.00 8761149.00 3544622.00 4684518.00 5412910.00 5685888.00 H 317579.00 5071431.00 8854270.00 5910143.00 5478528.00 2922598.00 9080201.00 3296317.00 1944448.00 2790725.00 3609875.00 3859454.00 Load time: 08/30/2013 10:01:43 AM Read time: 08/30/2013 10:01:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3201 Comment: Plate 11 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 20092462 cps Min counts: 16696 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2921563.00 4406086.00 6839908.00 6011994.00 8816131.00 8524043.00 6396280.00 9367432.00 10656497.00 6750104.00 7447756.00 6301039.00 B 8253081.00 7152019.00 9311327.00 7140329.00 5563144.00 5872850.00 199429.00 5141607.00 17177736.00 5468767.00 5690252.00 6530399.00 C 4895820.00 9202773.00 11475688.00 5138913.00 4334995.00 3242475.00 11639779.00 10738354.00 11128047.00 4070440.00 2917043.00 4673448.00 D 3852938.00 6546178.00 5809489.00 11165045.00 11476113.00 6438171.00 9218643.00 7119385.00 6877520.00 3743413.00 6940892.00 3614111.00 E 2251566.00 8500490.00 5354709.00 33392.00 6318016.00 20092462.00 4082787.00 7224451.00 6934674.00 5250724.00 6939057.00 6874974.00 F 2101902.00 6476498.00 5435659.00 4145990.00 6166402.00 7868506.00 5815529.00 5431906.00 7927535.00 48510.00 4099875.00 5493682.00 G 2269955.00 4425252.00 5344159.00 3156660.00 5285368.00 11058494.00 4917673.00 7349952.00 5228617.00 2914985.00 3050632.00 4006358.00 H 2188770.00 5485525.00 4229176.00 4819527.00 3581879.00 9634813.00 3995674.00 3535354.00 3534933.00 1701012.00 317409.00 3892506.00 Load time: 08/30/2013 10:04:01 AM Read time: 08/30/2013 10:04:01 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3202 Comment: Plate 12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14802864 cps Min counts: 31909 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4677981.00 2485297.00 5692830.00 4918299.00 4463042.00 12768221.00 6708841.00 4859399.00 6544208.00 3406003.00 63818.00 3222315.00 B 9434002.00 8904886.00 5018087.00 5835064.00 11543595.00 4963562.00 3727521.00 4603293.00 5112929.00 3994715.00 4054838.00 7022935.00 C 6360567.00 4408600.00 4141697.00 4467563.00 11803659.00 7999699.00 7791749.00 14086974.00 3837927.00 4003381.00 4948142.00 4800681.00 D 4823546.00 5808120.00 6276708.00 4576669.00 6456009.00 7455152.00 8324404.00 6313476.00 492025.00 5216218.00 3773411.00 4712765.00 E 4634615.00 4843651.00 7067203.00 5040713.00 5225927.00 5305790.00 8981741.00 14802864.00 4365159.00 4776255.00 3590535.00 5177964.00 F 5447787.00 4001230.00 9274155.00 2962681.00 3471230.00 6259967.00 9987730.00 4899805.00 4089847.00 4640984.00 1639599.00 5435125.00 G 3225380.00 3247197.00 755802.00 4264331.00 3720022.00 5539091.00 7203360.00 4489464.00 5077835.00 5286314.00 4961045.00 12536958.00 H 2579705.00 2723854.00 11503657.00 1766630.00 3733124.00 4468367.00 7615384.00 1635122.00 4834294.00 3907095.00 511660.00 6574534.00