Load time: 08/30/2013 10:07:42 AM Read time: 08/30/2013 10:07:42 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3203 Comment: Standards; blanks Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 27220282 cps Min counts: 1155 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25607210.00 25583274.00 27220282.00 17823.00 17813.00 2521.00 2434.00 2455.00 2352.00 2403.00 2437.00 2465.00 B 24171678.00 24288534.00 25160794.00 17672.00 16941.00 2391.00 2405.00 2426.00 2515.00 2395.00 2410.00 2422.00 C 15722506.00 16574748.00 16326570.00 17063.00 17990.00 2463.00 3040.00 2459.00 2643.00 2372.00 2356.00 2457.00 D 9252694.00 9901804.00 10230409.00 16972.00 28215.00 2470.00 2430.00 2484.00 2449.00 2531.00 2310.00 2583.00 E 5293433.00 5262725.00 6050334.00 15488.00 17808.00 2531.00 2405.00 2432.00 2422.00 2377.00 2420.00 2337.00 F 3171550.00 3116464.00 3081334.00 17546.00 17717.00 2449.00 2436.00 2501.00 2511.00 2467.00 2447.00 2428.00 G 2122726.00 2213806.00 2184502.00 17825.00 17872.00 2455.00 2472.00 2350.00 2531.00 2381.00 2472.00 2364.00 H 1145188.00 1164691.00 1232316.00 17381.00 17494.00 2344.00 2484.00 2432.00 2410.00 2414.00 2505.00 2356.00 Load time: 08/30/2013 10:10:56 AM Read time: 08/30/2013 10:10:56 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3204 Comment: Standards; blanks Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 9159382 cps Min counts: 11354 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1291889.00 8339965.00 3479159.00 2785910.00 996761.00 3539284.00 3440704.00 3074555.00 4882536.00 3854192.00 1321406.00 1742504.00 B 3949380.00 9159382.00 4975510.00 5472203.00 843068.00 4075795.00 2555922.00 3146408.00 3951799.00 8984086.00 3224789.00 3025525.00 C 4186631.00 4798449.00 5129020.00 6686359.00 7239972.00 3287424.00 3814520.00 3599631.00 5437977.00 5908608.00 3388880.00 362907.00 D 217097.00 4535651.00 4808244.00 6204648.00 5130677.00 4323400.00 3203058.00 142284.00 6026724.00 4950315.00 67963.00 4946829.00 E 2900565.00 47230.00 4682040.00 4773461.00 5470657.00 5493144.00 2859791.00 2774954.00 119203.00 3539600.00 2912506.00 3303857.00 F 838329.00 8587448.00 3084231.00 120327.00 3542001.00 4913392.00 1123769.00 69637.00 4201278.00 4389140.00 3192777.00 2928430.00 G 1475255.00 4302707.00 344259.00 3910199.00 1263838.00 3496475.00 3312957.00 3142633.00 5962084.00 3623770.00 2835610.00 3143928.00 H 3185001.00 2718039.00 22709.00 2492661.00 2537376.00 2335434.00 3128649.00 2668506.00 2857464.00 3655645.00 1056633.00 428742.00 Load time: 08/30/2013 10:13:32 AM Read time: 08/30/2013 10:13:32 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3205 Comment: Plate 8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 14148938 cps Min counts: 10031 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3101754.00 7372782.00 6244820.00 5036788.00 3603029.00 5930725.00 3654585.00 3508142.00 3592170.00 218793.00 1711162.00 4999356.00 B 20063.00 4572437.00 7543097.00 9434364.00 3213405.00 7666951.00 4065296.00 4421977.00 12525015.00 4272186.00 3392501.00 3672796.00 C 3525107.00 4146696.00 7058930.00 4895001.00 4440181.00 5816818.00 7173045.00 4765150.00 7626292.00 38577.00 3178840.00 3862613.00 D 3684217.00 7161655.00 5877720.00 9626343.00 8896885.00 14148938.00 10251945.00 5413094.00 5740387.00 4577948.00 4476211.00 3866055.00 E 3114838.00 6887437.00 5773999.00 7123736.00 6429699.00 3168474.00 5500414.00 2825587.00 589438.00 4444631.00 4109195.00 2885939.00 F 2689618.00 5408079.00 513866.00 3311465.00 5708928.00 2448610.00 4515551.00 3882715.00 5738063.00 2432373.00 4886533.00 2705763.00 G 1514329.00 4749896.00 4112133.00 5681917.00 4263833.00 4363588.00 5658829.00 3582217.00 8286595.00 2071032.00 3969624.00 3216178.00 H 1546939.00 3807932.00 3917416.00 3226125.00 3249767.00 4195660.00 3759349.00 2501435.00 1744522.00 2183468.00 4246975.00 2003032.00 Load time: 08/30/2013 10:15:52 AM Read time: 08/30/2013 10:15:52 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3206 Comment: Plate 10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 18493532 cps Min counts: 13007 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3657094.00 1789296.00 10799676.00 3374345.00 5556474.00 11882311.00 45602.00 3406945.00 3343165.00 7687370.00 26015.00 3368302.00 B 3384243.00 2308572.00 4308663.00 4984132.00 12126642.00 18493532.00 3980931.00 4525798.00 3505632.00 4271427.00 11372462.00 4068233.00 C 4530059.00 3608729.00 5598366.00 9158960.00 5744499.00 10488873.00 7692137.00 14957562.00 6984214.00 5925412.00 9912189.00 5491055.00 D 4685274.00 4540035.00 4118875.00 11984025.00 13559690.00 9491444.00 7131927.00 6604855.00 7955242.00 5482105.00 5863609.00 4188863.00 E 5685416.00 4602127.00 13351912.00 6400202.00 4931738.00 11710005.00 6136631.00 11059378.00 3885566.00 5573679.00 4961547.00 5820187.00 F 2847786.00 3938974.00 10771102.00 6059710.00 4693085.00 4814076.00 10250092.00 6870686.00 2280654.00 4684583.00 4987953.00 4267501.00 G 4580484.00 5289623.00 4170267.00 8205478.00 5896757.00 3406579.00 7380665.00 8131758.00 3419199.00 4450911.00 5172400.00 5477112.00 H 300892.00 4852243.00 8241942.00 5656893.00 5220397.00 2803909.00 8509293.00 3167248.00 1854100.00 2649393.00 3469745.00 3673527.00 Load time: 08/30/2013 10:18:18 AM Read time: 08/30/2013 10:18:18 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3207 Comment: Plate 10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 18738000 cps Min counts: 16187 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2782424.00 4138200.00 6330884.00 5668244.00 8309323.00 8022698.00 5953856.00 8964606.00 10271447.00 6405004.00 7120348.00 6015853.00 B 7711491.00 6737549.00 8771151.00 6790052.00 5207519.00 5622036.00 195361.00 4955599.00 16397395.00 5095111.00 5445625.00 6303685.00 C 4572911.00 8516322.00 10473511.00 4838667.00 4181558.00 3119257.00 11169711.00 10208437.00 10675066.00 3923264.00 2844185.00 4564391.00 D 3626982.00 6149515.00 5462225.00 10457781.00 10888113.00 6225328.00 8942085.00 6833836.00 6636384.00 3570744.00 6697472.00 3488270.00 E 2118834.00 7866394.00 5108916.00 32374.00 6097460.00 18738000.00 3915542.00 6788485.00 6668945.00 4999766.00 6833248.00 6573895.00 F 1986804.00 6061746.00 5203765.00 3907254.00 5970694.00 7629884.00 5490333.00 5246412.00 7558108.00 47676.00 3958578.00 5416012.00 G 2120005.00 4185329.00 5069794.00 3003488.00 4987044.00 10461088.00 4733186.00 7110717.00 5026423.00 2814646.00 2932400.00 3890345.00 H 2069445.00 5197427.00 3993409.00 4542555.00 3543361.00 9273339.00 3805669.00 3419788.00 3407588.00 1643514.00 300009.00 3573605.00 Load time: 08/30/2013 10:20:30 AM Read time: 08/30/2013 10:20:30 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3208 Comment: Plate 12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 14315302 cps Min counts: 30753 counts Well list: r0(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10) = cps(f2(a1:h3,a4:d4,a5:h5,a6:d6,a7:d7,e4,f4,g4,h4,h6,g6,f6,e6,e7,f7,g7,h7,h8,g8,f8,e8,d8,c8,a8,b8,a9,b9,c9,d9,e9,f9,g9:h10,a11:h12,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4589117.00 2422250.00 5348069.00 4816530.00 4169194.00 12265110.00 6510436.00 4707809.00 6286910.00 3299489.00 61507.00 3146832.00 B 8991006.00 8479931.00 5064844.00 5617978.00 11094310.00 4661776.00 3585972.00 4548437.00 4981098.00 3790114.00 3817970.00 6619063.00 C 6158484.00 4200677.00 3932359.00 4275477.00 11135709.00 7690646.00 7495023.00 13442810.00 3721980.00 3859758.00 4682068.00 4521184.00 D 4595892.00 5672699.00 6053565.00 4335869.00 6098678.00 7201287.00 7999494.00 6121441.00 475418.00 5068887.00 3650602.00 4560641.00 E 4605999.00 4612261.00 6701260.00 4839464.00 5024387.00 5013692.00 8608775.00 14315302.00 4167749.00 4655350.00 3447981.00 5077577.00 F 5226481.00 3875398.00 9257982.00 2926044.00 3300189.00 5876023.00 9312723.00 4684416.00 3933976.00 4494993.00 1612613.00 5227823.00 G 3104751.00 3265166.00 716710.00 4110067.00 3500669.00 5186933.00 6808631.00 4250568.00 4817612.00 4988800.00 4729576.00 11907087.00 H 2459116.00 2656468.00 11087646.00 1696428.00 3577374.00 4243025.00 7325223.00 1576453.00 4605865.00 3748732.00 491442.00 6351145.00