Load time: 09/06/2013 02:24:32 PM Read time: 09/06/2013 02:24:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3244 Comment: Standard; Plate8; Blank Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65373784 cps Min counts: 1095 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65373784.00 59433496.00 64414696.00 2009304.00 154897.00 10520.00 7328.00 2379.00 2190.00 2304.00 2323.00 2360.00 B 38846700.00 37951424.00 36062924.00 4484530.00 163756.00 9481.00 7347.00 2530.00 2568.00 2360.00 2304.00 2360.00 C 22134352.00 21761530.00 20644210.00 3559599.00 158996.00 8952.00 12088.00 2304.00 2398.00 2360.00 2360.00 2417.00 D 14604335.00 14776498.00 16250241.00 317596.00 155313.00 9066.00 9368.00 2436.00 2323.00 2342.00 2323.00 2304.00 E 7998897.00 7758766.00 7760385.00 2862777.00 155011.00 23194.00 8801.00 2417.00 2398.00 2304.00 2398.00 2398.00 F 4905816.00 4656842.00 4530252.00 588068.00 157183.00 12484.00 25857.00 2436.00 2417.00 2379.00 2417.00 2379.00 G 2778306.00 2744840.00 2633655.00 1338174.00 157598.00 15809.00 8197.00 2455.00 2436.00 2474.00 2417.00 2285.00 H 1502591.00 1488595.00 1612007.00 3146176.00 153840.00 11729.00 8121.00 2417.00 2493.00 2342.00 2530.00 2417.00 Load time: 09/06/2013 02:27:14 PM Read time: 09/06/2013 02:27:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3245 Comment: Plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13114829 cps Min counts: 87478 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4237091.00 1066153.00 4037360.00 2175319.00 1206187.00 7256415.00 2156930.00 7200581.00 1981109.00 329080.00 5644859.00 4307691.00 B 8431756.00 2764163.00 9966464.00 201286.00 7378631.00 4811309.00 2424958.00 12355068.00 5693501.00 1558106.00 6079804.00 8487546.00 C 220589.00 2987499.00 174956.00 7031178.00 5526042.00 4910368.00 7586318.00 7259799.00 5036373.00 7752617.00 196262.00 4803381.00 D 178923.00 12685740.00 3965270.00 4879770.00 4321821.00 3890540.00 7455382.00 6535301.00 9232716.00 4053455.00 9204617.00 6156518.00 E 1191549.00 8004625.00 241233.00 3249287.00 1222279.00 3533102.00 213619.00 9318223.00 4288965.00 4318757.00 4131751.00 4254686.00 F 5952188.00 1263775.00 2911539.00 5263765.00 473080.00 215281.00 778701.00 1316566.00 13114829.00 5779858.00 5818626.00 4676058.00 G 3906662.00 1500381.00 3978284.00 4224342.00 5212538.00 3553986.00 970374.00 7067359.00 5336801.00 3790557.00 3883390.00 6379108.00 H 309078.00 329080.00 7026593.00 222440.00 4491359.00 5194109.00 190331.00 4817433.00 4713013.00 211674.00 3422179.00 4376664.00 Load time: 09/06/2013 02:29:47 PM Read time: 09/06/2013 02:29:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3246 Comment: Plate 13 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 5247392 cps Min counts: 87365 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1476394.00 174730.00 1651378.00 1833039.00 1376969.00 1965843.00 2737136.00 778550.00 1215253.00 2810496.00 1403676.00 526286.00 B 968315.00 2490984.00 2512257.00 2114112.00 2794550.00 3894773.00 2166445.00 2338481.00 2115302.00 2373809.00 2389455.00 2103489.00 C 1247022.00 5216113.00 3470724.00 2971118.00 2159159.00 1526522.00 1947705.00 1682219.00 2127623.00 1967944.00 1866884.00 945517.00 D 1355362.00 1826980.00 3417928.00 2133965.00 1900525.00 1255767.00 3638390.00 980252.00 2173811.00 3530847.00 2148295.00 2139408.00 E 1918314.00 1459697.00 2246311.00 2957794.00 3479420.00 1565538.00 5247392.00 1891501.00 3609680.00 1918255.00 1446910.00 201191.00 F 2636403.00 3032345.00 3876366.00 3441121.00 3148366.00 1905904.00 3714140.00 2709989.00 2296758.00 2375642.00 1191228.00 3864107.00 G 1937077.00 3613597.00 5170994.00 4839652.00 4968201.00 2461489.00 3304478.00 2759981.00 2876572.00 3105892.00 3410200.00 2073353.00 H 3846189.00 2599878.00 2333365.00 4844935.00 2592432.00 401344.00 4577130.00 1943979.00 1711004.00 2494333.00 1431932.00 1176080.00 Load time: 09/06/2013 02:32:17 PM Read time: 09/06/2013 02:32:17 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3247 Comment: Plate 14 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 7221457 cps Min counts: 98263 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 515369.00 882244.00 2722647.00 2162587.00 3754339.00 1361160.00 3052949.00 2510188.00 4763334.00 2943263.00 3611568.00 241894.00 B 1415330.00 2553004.00 3259735.00 2190654.00 4599096.00 4684016.00 2775276.00 2765889.00 2648966.00 3939165.00 3895979.00 423348.00 C 1123081.00 2940935.00 2986307.00 2272963.00 2832134.00 2461890.00 2451972.00 1697228.00 3234689.00 5248560.00 3347210.00 4917687.00 D 2272752.00 3591417.00 2499183.00 3725756.00 3769802.00 3017107.00 2628390.00 2385202.00 3134784.00 4677025.00 5142360.00 4777663.00 E 3279299.00 4310314.00 3752241.00 2723454.00 3820770.00 1327332.00 1659945.00 1349884.00 1382560.00 6025785.00 7221457.00 4351784.00 F 2247664.00 3616312.00 2446418.00 4029672.00 4704101.00 5524593.00 3857336.00 755356.00 1785718.00 5360820.00 3851224.00 449036.00 G 3585972.00 5409391.00 2854067.00 3398801.00 5334167.00 3374860.00 2005733.00 467130.00 2523312.00 2278740.00 196526.00 2669766.00 H 5240844.00 3028993.00 3409546.00 1297962.00 4209008.00 1459263.00 1528996.00 3803888.00 2882648.00 2865541.00 3479424.00 4915766.00 Load time: 09/06/2013 02:34:50 PM Read time: 09/06/2013 02:34:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3248 Comment: Plate 15 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9864458 cps Min counts: 509608 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1019217.00 1911959.00 1782514.00 1545409.00 2703829.00 3655958.00 2372783.00 1315225.00 1740674.00 1783912.00 2593836.00 3346301.00 B 1583551.00 3511687.00 1561693.00 2123094.00 2692705.00 2620509.00 1253330.00 2423752.00 1834642.00 2597566.00 2656747.00 2823982.00 C 3266708.00 2951829.00 1896081.00 2314295.00 4003182.00 2695640.00 1616020.00 1862009.00 1175664.00 2223894.00 2386784.00 2435249.00 D 2101305.00 4293724.00 2352033.00 1894970.00 3750157.00 2172443.00 2294702.00 2893769.00 1972591.00 4069244.00 3080193.00 2981644.00 E 3213621.00 2628013.00 2771781.00 3664641.00 3703259.00 2179320.00 1879601.00 2737437.00 2167556.00 3209106.00 2697696.00 2144678.00 F 2684163.00 5511181.00 3279089.00 3008681.00 5440907.00 5881251.00 3412023.00 4705011.00 4138341.00 2572032.00 3782376.00 3592479.00 G 3475536.00 2363745.00 4867391.00 9215780.00 5270467.00 9864458.00 1596030.00 7041712.00 3403386.00 3126195.00 3524336.00 5728259.00 H 2342080.00 6628429.00 3162656.00 3436563.00 7967733.00 1620141.00 3659394.00 3838174.00 2606579.00 4459319.00 2391827.00 2326825.00 Load time: 09/06/2013 02:37:13 PM Read time: 09/06/2013 02:37:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3249 Comment: Plate 16 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 8787127 cps Min counts: 71253 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1464362.00 2270180.00 2129021.00 2154129.00 2250254.00 1968170.00 3850632.00 1942210.00 183607.00 2207421.00 150497.00 6545469.00 B 3137243.00 7365466.00 3417721.00 2161148.00 2378670.00 3251146.00 3345300.00 2456993.00 6320488.00 2508318.00 3985249.00 3439287.00 C 3267524.00 3552928.00 3328745.00 2050622.00 2160562.00 3201984.00 1821421.00 3900408.00 3839003.00 142507.00 3340259.00 4240974.00 D 1612527.00 1547391.00 2223221.00 2126002.00 3609985.00 2669538.00 2535207.00 3300595.00 2203405.00 3329528.00 3238957.00 4100113.00 E 2951561.00 1980321.00 1963032.00 2623608.00 4142409.00 3734732.00 3815185.00 5126913.00 4621322.00 3333984.00 1887168.00 3279607.00 F 162226.00 3807625.00 2526870.00 2310789.00 6717280.00 5980121.00 6470735.00 5074073.00 3077724.00 4394110.00 4152479.00 3518777.00 G 3479012.00 4601703.00 5576763.00 2651121.00 3264880.00 5222296.00 1891394.00 3859492.00 4711223.00 3359331.00 8787127.00 2047586.00 H 3574896.00 4184868.00 4705635.00 3027698.00 1426190.00 4017257.00 2207775.00 4827717.00 3978801.00 298293.00 3797927.00 4194775.00