Load time: 08/29/2012 09:07:35 AM Read time: 08/29/2012 09:07:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2853 Comment: poplar rescan plate 1, IMH Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46753980 cps Min counts: 55956 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 46753980.00 44098788.00 45447432.00 111913.00 B 28329066.00 27698824.00 29291856.00 112706.00 C 18869878.00 18031552.00 18291432.00 112687.00 D 10916294.00 10501534.00 11230694.00 113349.00 E 6386582.00 6072259.00 6477029.00 112555.00 F 3597160.00 3235832.00 3639062.00 112555.00 G 2168739.00 1926195.00 2143826.00 116674.00 H 1465397.00 1422664.00 1445712.00 113727.00 Load time: 08/29/2012 09:09:39 AM Read time: 08/29/2012 09:09:39 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2854 Comment: poplar rescan plate 1b, IMH Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3116176 cps Min counts: 74394 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 148789.00 281370.00 327352.00 259928.00 241452.00 343296.00 234387.00 326143.00 364871.00 308404.00 348775.00 344543.00 B 184740.00 448126.00 311842.00 311030.00 399612.00 312465.00 398384.00 367100.00 386766.00 265048.00 182605.00 413913.00 C 154438.00 287680.00 524939.00 384763.00 343126.00 411854.00 470059.00 485833.00 401199.00 449580.00 478012.00 534517.00 D 212643.00 297352.00 634037.00 433522.00 416577.00 341011.00 410456.00 530247.00 397043.00 367515.00 462615.00 727437.00 E 1487746.00 1986052.00 2403672.00 2109901.00 2854149.00 2333400.00 2508482.00 3116176.00 2744992.00 2236879.00 1665419.00 1835113.00 F 1109650.00 1258346.00 2436155.00 1642876.00 2261359.00 2039324.00 2244843.00 2262305.00 2319354.00 1917556.00 1847515.00 1758077.00 G 1018272.00 1478205.00 2089068.00 2108162.00 2174693.00 2051542.00 1921599.00 2515058.00 2657382.00 1902154.00 2141042.00 1650291.00 H 1303818.00 1843039.00 1638558.00 1550762.00 1876396.00 1627031.00 2066919.00 1925348.00 1507393.00 1436738.00 1446921.00 1783329.00