Load time: 09/14/2010 09:55:40 AM Read time: 09/14/2010 09:55:40 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1036 Comment: Standard Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 74328608 cps Min counts: 99793 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 74328608.00 73984464.00 72276904.00 202518.00 B 41438816.00 40521880.00 44127164.00 202366.00 C 25737762.00 23605472.00 25485734.00 199853.00 D 14799054.00 13376562.00 14889822.00 202651.00 E 8845625.00 8856817.00 8570663.00 200557.00 F 5321524.00 5340655.00 5600798.00 208781.00 G 3191632.00 2940205.00 3247247.00 199586.00 H 2078752.00 1974765.00 1945533.00 205316.00 Load time: 09/14/2010 09:57:44 AM Read time: 09/14/2010 09:57:44 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1038 Comment: Cowpea Plate 1 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 12391135 cps Min counts: 284121 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1015771.00 568243.00 1816159.00 2482748.00 3332234.00 8950428.00 8803610.00 3934828.00 5787736.00 6077455.00 5596187.00 2122809.00 B 2119633.00 1866460.00 3360309.00 823266.00 8295990.00 7582195.00 6332178.00 7133169.00 7390837.00 7108329.00 1885568.00 4616377.00 C 1534232.00 2415428.00 1500727.00 1724765.00 7401311.00 6391241.00 10326777.00 8072277.00 6581931.00 9957140.00 2979055.00 3808133.00 D 2473546.00 1917155.00 3487149.00 1137172.00 2880712.00 4744777.00 10755595.00 7609906.00 9159069.00 5199889.00 1035799.00 813196.00 E 2261746.00 1348581.00 2417980.00 2164759.00 7614931.00 5172901.00 12391135.00 7895203.00 5687953.00 3897599.00 1163691.00 2779476.00 F 1615420.00 1048649.00 9192457.00 620196.00 3965950.00 7986605.00 11108324.00 5234383.00 6442329.00 1861341.00 3701251.00 3384348.00 G 2124930.00 947980.00 3508874.00 2151815.00 4090467.00 6623133.00 12354363.00 7212341.00 5016338.00 1302149.00 2167776.00 3810522.00 H 1350256.00 1766819.00 4184866.00 4821827.00 3969265.00 4135994.00 7671938.00 3523005.00 4956340.00 2113571.00 1655866.00 2080348.00 Load time: 09/14/2010 10:00:06 AM Read time: 09/14/2010 10:00:06 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1039 Comment: Cowpea Plate 2 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9661326 cps Min counts: 400182 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2254536.00 1069057.00 1780021.00 5023599.00 2178759.00 8501451.00 5923693.00 3022542.00 7404881.00 4158892.00 3682923.00 2313402.00 B 1711165.00 800365.00 4610059.00 3217384.00 994964.00 3729051.00 3901693.00 3758190.00 3222073.00 5356839.00 3820048.00 3739522.00 C 1953222.00 3151984.00 9360225.00 3202320.00 4272731.00 5572926.00 2418296.00 2254198.00 3750462.00 2375194.00 3316115.00 2821022.00 D 3155685.00 1955509.00 4559460.00 2815025.00 5089262.00 6195960.00 2655430.00 5122895.00 4025100.00 2144760.00 2429513.00 5088614.00 E 1797177.00 3096231.00 5635426.00 5804002.00 1609153.00 6364361.00 2179080.00 6277333.00 2258275.00 2605390.00 3741289.00 2584642.00 F 1502212.00 2303533.00 3392841.00 3762522.00 5603812.00 4732351.00 2008435.00 2952675.00 3204798.00 2848998.00 3627616.00 3880679.00 G 1599394.00 1228532.00 3243099.00 3938744.00 2612596.00 8921855.00 2475965.00 2912944.00 2270239.00 1874051.00 1734456.00 2077081.00 H 1348981.00 4026834.00 3114425.00 9661326.00 2641984.00 5471989.00 2752358.00 8200395.00 2480333.00 2127940.00 1568621.00 2763311.00 Load time: 09/14/2010 10:02:35 AM Read time: 09/14/2010 10:02:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1040 Comment: Cowpea Plate 3 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9384015 cps Min counts: 160255 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3358179.00 785040.00 4685706.00 2292533.00 1957843.00 2277039.00 2880066.00 1795718.00 5200198.00 5410770.00 1388502.00 2044818.00 B 7189048.00 573402.00 1683562.00 4508174.00 5685680.00 4919493.00 8502146.00 2519292.00 7536933.00 4272268.00 2568106.00 2535156.00 C 2966516.00 3162978.00 3703378.00 5434273.00 2984912.00 5600186.00 5235926.00 3529720.00 3468905.00 4059305.00 4399617.00 1851147.00 D 2494399.00 538012.00 320511.00 2335923.00 3569704.00 8067867.00 9384015.00 3677939.00 4781585.00 3234469.00 4220121.00 2254406.00 E 2354551.00 1916383.00 436162.00 2657286.00 3418820.00 2353425.00 6194183.00 4843460.00 3746687.00 1953281.00 4882376.00 1411118.00 F 1836701.00 2059519.00 816851.00 1214273.00 3073975.00 4818130.00 3510875.00 1764762.00 1793240.00 3163447.00 2250584.00 2154145.00 G 1798403.00 657813.00 1907444.00 2390733.00 1034694.00 6937012.00 2123615.00 2342888.00 4061489.00 2554705.00 4290897.00 3089713.00 H 1637138.00 1656485.00 963038.00 2533853.00 842570.00 3653820.00 2364937.00 1502783.00 2187909.00 2417934.00 3236307.00 2705673.00 Load time: 09/14/2010 10:05:13 AM Read time: 09/14/2010 10:05:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1041 Comment: Cowpea Plate 4 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9618342 cps Min counts: 460130 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2206838.00 2317747.00 2000048.00 3224052.00 4618272.00 3697475.00 7084607.00 2014705.00 4918943.00 2667661.00 3920107.00 3376880.00 B 1876564.00 2025845.00 3454145.00 5671059.00 2648964.00 2005911.00 6223569.00 5896482.00 5246834.00 7141452.00 6713913.00 3227968.00 C 1895726.00 3758590.00 6711624.00 4368864.00 4519998.00 1877757.00 3722191.00 2062992.00 4347560.00 3024035.00 7499465.00 2988337.00 D 3653274.00 1564453.00 4139049.00 5554954.00 7555775.00 2269202.00 920261.00 6693896.00 4003755.00 2471225.00 5507495.00 2004085.00 E 2325958.00 2582732.00 6340462.00 4231919.00 4533400.00 4909299.00 1664749.00 3993304.00 3177118.00 2140821.00 2402969.00 4515886.00 F 2391782.00 1484374.00 3635258.00 5255069.00 961439.00 1416925.00 2670252.00 9618342.00 2179258.00 5576010.00 1938563.00 2430385.00 G 2826545.00 1810844.00 1453172.00 2554590.00 1567917.00 1381858.00 3044240.00 6205201.00 3084479.00 2348699.00 1733847.00 2116326.00 H 2959018.00 1711137.00 2891221.00 1638074.00 1780056.00 1677743.00 2609023.00 3090173.00 3695394.00 2010137.00 2908482.00 2893461.00