Load time: 10/01/2010 11:23:38 AM Read time: 10/01/2010 11:23:38 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1073 Comment: standard, tyson,jenny Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 58382516 cps Min counts: 65770 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 55590800.00 52081496.00 58382516.00 139117.00 226437.00 213454.00 226836.00 136185.00 136357.00 134091.00 133749.00 132016.00 B 35049924.00 35456668.00 35171504.00 132987.00 544229.00 509488.00 520948.00 136281.00 137822.00 132245.00 135081.00 133711.00 C 20847330.00 21211538.00 20904676.00 134358.00 729243.00 654944.00 697414.00 134758.00 135367.00 133615.00 135062.00 134168.00 D 12878099.00 12094859.00 11752621.00 135652.00 695396.00 644208.00 626085.00 134720.00 134320.00 133558.00 135500.00 135081.00 E 6977234.00 7241969.00 6837143.00 135709.00 907042.00 814469.00 855225.00 135215.00 137575.00 135043.00 135576.00 132968.00 F 4543811.00 4529840.00 4255565.00 1448226.00 954899.00 875994.00 875804.00 135291.00 135728.00 136262.00 137213.00 135786.00 G 2862346.00 2648622.00 3080349.00 1435928.00 974488.00 932874.00 1009819.00 136909.00 144409.00 133768.00 135081.00 135443.00 H 1692249.00 1718210.00 1772158.00 1435491.00 355027.00 324569.00 331555.00 136471.00 136223.00 135614.00 131540.00 134168.00 Load time: 10/01/2010 11:26:22 AM Read time: 10/01/2010 11:26:22 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1074 Comment: 1-36220 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 96696016 cps Min counts: 779727 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33376182.00 28063506.00 30761762.00 33765112.00 30658590.00 34445284.00 36645296.00 35973096.00 34721380.00 33862040.00 34233864.00 31286344.00 B 32260810.00 39081344.00 27229312.00 37816528.00 35929496.00 37092972.00 35280360.00 37400956.00 33659444.00 30307874.00 32768826.00 34312052.00 C 31541232.00 35896088.00 35723408.00 35919736.00 41662132.00 36616044.00 34905572.00 39503280.00 37364216.00 32190224.00 35949216.00 37030436.00 D 31629260.00 34004692.00 34380436.00 35735032.00 43275652.00 41530100.00 35420592.00 42366832.00 40179928.00 31706686.00 44347056.00 41686412.00 E 32436214.00 35036696.00 38590828.00 31270910.00 38597896.00 39409444.00 37879172.00 40428256.00 35995520.00 38595224.00 33127744.00 41216616.00 F 32008264.00 35849544.00 36672008.00 28953112.00 35665072.00 37324868.00 35721604.00 35950040.00 34072156.00 29182844.00 33520382.00 36467228.00 G 30233604.00 31441264.00 30715232.00 27203092.00 33885292.00 32505446.00 32517782.00 34527656.00 30198590.00 27814138.00 35773396.00 96696016.00 H 24819128.00 30335702.00 31260882.00 31404542.00 32696960.00 41011532.00 31031588.00 35078312.00 42005528.00 27400108.00 31429354.00 87857624.00 Load time: 10/01/2010 11:28:16 AM Read time: 10/01/2010 11:28:16 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1075 Comment: 2-31900 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36672008 cps Min counts: 779727 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20630442.00 21035176.00 23439178.00 36672008.00 25977758.00 24923214.00 30542102.00 20851456.00 21656214.00 18505770.00 20228496.00 25792492.00 B 23383644.00 22406290.00 22001906.00 34640552.00 26493504.00 26628042.00 27461002.00 21357606.00 21277000.00 20970004.00 21635424.00 26990750.00 C 22474682.00 23771750.00 25367804.00 28674364.00 28711002.00 23370750.00 26270704.00 21601616.00 23047660.00 20977114.00 21096014.00 33646948.00 D 21867020.00 26326230.00 25842840.00 28341884.00 32083334.00 26466254.00 31413398.00 24096682.00 23143706.00 23026082.00 29074786.00 30356726.00 E 22425234.00 24220486.00 27074442.00 28248546.00 25312464.00 24269258.00 24468956.00 23988742.00 23543924.00 23800482.00 25625838.00 31786438.00 F 22481682.00 22428268.00 21449728.00 19362158.00 23233286.00 23766534.00 21839338.00 20239944.00 20336228.00 20192872.00 24851402.00 28608610.00 G 20324092.00 20417440.00 20767704.00 15741898.00 21302172.00 22261906.00 21165418.00 19518566.00 17751988.00 18717850.00 22365350.00 21625280.00 H 20643280.00 20441792.00 19111254.00 11722248.00 21715138.00 25276114.00 22139842.00 19829398.00 19198874.00 20426050.00 27677006.00 22159348.00 Load time: 10/01/2010 11:30:13 AM Read time: 10/01/2010 11:30:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1076 Comment: 3-03816 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38998080 cps Min counts: 779727 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27237682.00 14763039.00 22288952.00 17495354.00 25356418.00 25031306.00 23766822.00 29014308.00 24384092.00 29878746.00 23200656.00 33568292.00 B 23309758.00 17923826.00 23419254.00 21026098.00 30135098.00 25399620.00 29581750.00 33417518.00 27524008.00 29236132.00 22580186.00 38998080.00 C 22912142.00 25255808.00 24861624.00 24719986.00 28633718.00 25085180.00 30316476.00 32475932.00 23312194.00 29008048.00 25527092.00 35816776.00 D 24935644.00 22537434.00 22144938.00 24365576.00 27512744.00 31826918.00 28104878.00 35809208.00 28461368.00 26956688.00 26149036.00 35470544.00 E 28777334.00 19588292.00 20867414.00 26223084.00 28982280.00 29483430.00 29916230.00 30011340.00 32588318.00 21357194.00 22029254.00 34210588.00 F 21944254.00 14196122.00 17753806.00 21671382.00 22511930.00 25224434.00 29718176.00 31087886.00 23298612.00 23431640.00 25204458.00 30316122.00 G 22536132.00 14762619.00 37226320.00 17624190.00 23067908.00 24829244.00 25381512.00 29326080.00 20536062.00 20606780.00 24795922.00 27902124.00 H 21862300.00 22069346.00 14674355.00 22393292.00 20560974.00 21758830.00 23953296.00 23028394.00 16572427.00 21923030.00 24621786.00 25103594.00 Load time: 10/01/2010 11:30:13 AM Read time: 10/01/2010 11:30:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1076 Comment: 3-03816 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38998080 cps Min counts: 779727 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27237682.00 14763039.00 22288952.00 17495354.00 25356418.00 25031306.00 23766822.00 29014308.00 24384092.00 29878746.00 23200656.00 33568292.00 B 23309758.00 17923826.00 23419254.00 21026098.00 30135098.00 25399620.00 29581750.00 33417518.00 27524008.00 29236132.00 22580186.00 38998080.00 C 22912142.00 25255808.00 24861624.00 24719986.00 28633718.00 25085180.00 30316476.00 32475932.00 23312194.00 29008048.00 25527092.00 35816776.00 D 24935644.00 22537434.00 22144938.00 24365576.00 27512744.00 31826918.00 28104878.00 35809208.00 28461368.00 26956688.00 26149036.00 35470544.00 E 28777334.00 19588292.00 20867414.00 26223084.00 28982280.00 29483430.00 29916230.00 30011340.00 32588318.00 21357194.00 22029254.00 34210588.00 F 21944254.00 14196122.00 17753806.00 21671382.00 22511930.00 25224434.00 29718176.00 31087886.00 23298612.00 23431640.00 25204458.00 30316122.00 G 22536132.00 14762619.00 37226320.00 17624190.00 23067908.00 24829244.00 25381512.00 29326080.00 20536062.00 20606780.00 24795922.00 27902124.00 H 21862300.00 22069346.00 14674355.00 22393292.00 20560974.00 21758830.00 23953296.00 23028394.00 16572427.00 21923030.00 24621786.00 25103594.00