Load time: 10/14/2010 12:22:08 PM Read time: 10/14/2010 12:22:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1096 Comment: Standards (C1-3), Cowpea (C10-12) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 82262144 cps Min counts: 913 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 82262144.00 47303196.00 41795140.00 1845.00 1960.00 1979.00 1979.00 2036.00 1902.00 4773637.00 2883107.00 1757122.00 B 31861916.00 26015266.00 25089620.00 1902.00 2036.00 1941.00 2112.00 2036.00 2017.00 4414684.00 4006350.00 2953788.00 C 21646864.00 14198377.00 9822491.00 1941.00 2036.00 1864.00 2169.00 1941.00 1979.00 5684244.00 4662755.00 3553027.00 D 12770502.00 6461948.00 6447495.00 1922.00 1864.00 2055.00 1960.00 1826.00 2017.00 5776523.00 4792042.00 4637345.00 E 4196351.00 4221050.00 4280884.00 2055.00 1960.00 2036.00 1960.00 2074.00 1902.00 4896474.00 7489924.00 4741980.00 F 2529684.00 4781644.00 2607725.00 1922.00 1998.00 2131.00 1941.00 1941.00 2226.00 4472966.00 3533054.00 3715973.00 G 2330914.00 2432931.00 2257320.00 1960.00 1979.00 2131.00 1922.00 2112.00 2131.00 4667228.00 3965125.00 1998.00 H 1550884.00 1477257.00 1512291.00 1998.00 1979.00 2017.00 2036.00 1922.00 1845.00 5000681.00 4358678.00 2188.00 Load time: 10/14/2010 12:24:32 PM Read time: 10/14/2010 12:24:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1097 Comment: Cowpea P#1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28219260 cps Min counts: 2588 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28219260.00 15222177.00 14302090.00 15111333.00 14368845.00 13413526.00 7770772.00 7032106.00 6143154.00 3922668.00 3706857.00 3594988.00 B 19680720.00 14935889.00 14680965.00 17432118.00 14119087.00 14054677.00 7855548.00 6931384.00 7606268.00 3751658.00 3802892.00 3728856.00 C 18109570.00 17646452.00 18771974.00 14199747.00 15045471.00 15756989.00 13416991.00 7995687.00 8394668.00 4742580.00 3243996.00 5355750.00 D 17634198.00 17237974.00 19564880.00 14740907.00 16720080.00 16995288.00 8990679.00 8357584.00 10146202.00 7153999.00 5100691.00 3874511.00 E 18709610.00 16759699.00 19711128.00 15619167.00 15696795.00 15835964.00 8997758.00 9035117.00 8121578.00 5307022.00 4640111.00 4709239.00 F 16657373.00 15420787.00 16268817.00 14170861.00 15793545.00 2872858.00 9072744.00 8326969.00 9265710.00 4702041.00 4561126.00 3495463.00 G 16702740.00 15341177.00 8373.00 11507100.00 12790011.00 6584.00 8750041.00 7618080.00 6584.00 3831728.00 3994690.00 5176.00 H 19899990.00 15639632.00 6298.00 12301064.00 13733475.00 5442.00 9092164.00 7481751.00 26775.00 4340410.00 4480317.00 6964.00 Load time: 10/14/2010 12:26:27 PM Read time: 10/14/2010 12:26:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1098 Comment: Cowpea P#2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 52452964 cps Min counts: 2825 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26980878.00 21291382.00 19116384.00 19908838.00 22193688.00 25750386.00 18077820.00 18630904.00 19215868.00 11008437.00 17411948.00 14280028.00 B 26054222.00 19334458.00 17298112.00 22828538.00 21546336.00 25779340.00 25655122.00 10733279.00 20699176.00 12450113.00 18328966.00 7822131.00 C 24336054.00 21724874.00 28525460.00 21465166.00 25958084.00 25193256.00 25411404.00 16018372.00 39676200.00 12330292.00 7826695.00 11939538.00 D 24730532.00 23417678.00 27518102.00 24435078.00 27255892.00 24619690.00 18042212.00 21701348.00 37378224.00 15016949.00 13061667.00 10880922.00 E 29504690.00 23191652.00 28917120.00 22862552.00 29485496.00 26649528.00 21117578.00 21455420.00 23299868.00 15561698.00 20993024.00 11415339.00 F 25423500.00 29182578.00 29395868.00 32732410.00 25062676.00 27227332.00 52452964.00 25688350.00 16336981.00 12774729.00 19338968.00 10699451.00 G 27122170.00 25561902.00 6451.00 23361738.00 26925330.00 16651.00 41794236.00 18793290.00 7250.00 20004076.00 13794432.00 144970.00 H 24602516.00 24517392.00 7535.00 22353056.00 30130722.00 7098.00 30674504.00 32748776.00 5651.00 30943252.00 20198934.00 145978.00 Load time: 10/14/2010 12:28:18 PM Read time: 10/14/2010 12:28:18 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1099 Comment: Cowpea P#3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28339506 cps Min counts: 88822 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24489352.00 27216588.00 27406928.00 18230242.00 17417940.00 14669747.00 19100412.00 26052916.00 13496209.00 13614737.00 18592022.00 9238168.00 B 22625900.00 21823718.00 20383546.00 18451188.00 19422186.00 15322479.00 27795214.00 27121228.00 14677731.00 21463808.00 17270440.00 10098747.00 C 22229512.00 23320990.00 27003870.00 16998030.00 21742936.00 16632455.00 22143816.00 24192318.00 18949592.00 12868454.00 13016561.00 13412812.00 D 24391418.00 22520978.00 24918218.00 20036572.00 13952460.00 16133819.00 22540122.00 17301566.00 17730828.00 19099428.00 24319804.00 10941313.00 E 22213042.00 25753874.00 25715214.00 15478386.00 17302298.00 16902306.00 28339506.00 23222190.00 18822242.00 20716724.00 14741075.00 18114790.00 F 24623112.00 17040580.00 16462959.00 13051257.00 16921790.00 14987296.00 24397372.00 25269540.00 17387828.00 17213306.00 16168490.00 12274778.00 G 18435218.00 17901286.00 181793.00 13736404.00 14288404.00 180765.00 22835494.00 20177392.00 180879.00 10747989.00 8021359.00 177911.00 H 19010178.00 20177340.00 223088.00 13352086.00 15761099.00 182135.00 22496992.00 20062410.00 178519.00 12771904.00 10948028.00 177644.00