Load time: 10/18/2010 14:55 Read time: 10/18/2010 14:55 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1105 Comment: "standards, dario" Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 67748416 cps Min counts: 904 counts Well list: r0(a1:h9) = cps(f2(a1:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 67748416 66157436 66684712 115027 1941 6414503 4534235 3115036 21294952 B 46343984 42622612 40465040 111962 1960 6507256 4873447 4724930 18510202 C 28257022 24265476 23745654 111048 1808 6736588 5569248 4565742 20257698 D 13799434 13978053 14031675 109316 1960 7462922 6734551 7993466 2189 E 9951732 9614255 9349622 108611 1998 6937857 6805456 8940308 2018 F 5341465 5175792 5387531 107127 1979 6258721 4328617 6147493 2170 G 2651963 2793481 2832650 108916 1960 6460975 5245561 2075 1941 H 1941385 1916485 1943405 106194 1884 6844492 6083539 1941 2094 Load time: 10/18/2010 14:57 Read time: 10/18/2010 14:57 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1106 Comment: helen Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59143244 cps Min counts: 779797 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 50713604 59143244 53963752 29564222 31980288 22229128 30166610 31347140 21968300 29123450 31782816 21573018 B 32432384 34778088 39022860 24956724 27780964 30336540 25399834 27146630 29469034 24906268 27238800 29276304 C 18815152 19161346 21587710 27537178 32406372 25864454 28200514 32517454 26005482 28876374 31159628 25195078 D 10913864 11151964 11955175 23140358 26703298 23700114 23638258 26166920 23877694 23403154 28101428 23484772 E 8738366 6788808 7204626 26573898 29730368 18206174 26776102 30592542 18816060 26566476 31243902 18519170 F 4872122 5472822 4632314 24360458 33437864 19794784 24187352 32512166 19453604 24387276 33687772 19556646 G 2659382 3209292 2849205 13107872 34200608 23099986 13494439 32817562 21634550 13634427 33708736 22269248 H 1569007 1609035 1596582 31536794 30379800 14781999 31722954 30806338 14276362 31842258 31502016 14734433 Load time: 10/20/2010 09:56:33 AM Read time: 10/20/2010 09:56:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1109 Comment: helen Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15403271 cps Min counts: 30005 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2537993.00 9273365.00 6652380.00 8377038.00 2449674.00 15403271.00 1363471.00 11042844.00 11776572.00 2524173.00 374291.00 2808098.00 B 2141263.00 6430668.00 11250847.00 4650987.00 6453845.00 3523899.00 2418365.00 10535507.00 4107984.00 1583378.00 2066797.00 4042165.00 C 677947.00 4147540.00 7378785.00 7677169.00 8055887.00 60011.00 2155510.00 10431615.00 4725586.00 2786514.00 2275772.00 4394867.00 D 1399665.00 61134.00 2614093.00 6343887.00 3305623.00 3381293.00 1812820.00 2132642.00 1506341.00 1197526.00 4728712.00 3358680.00 E 1905223.00 1775260.00 1560000.00 7028216.00 8406994.00 5091978.00 1887517.00 5232781.00 1114839.00 456483.00 528108.00 5726163.00 F 2283865.00 11734997.00 11324515.00 1839997.00 8701438.00 9155985.00 6734554.00 2553767.00 2849532.00 3278966.00 11684937.00 7550428.00 G 2954199.00 8093544.00 7672743.00 2947667.00 3363993.00 4671678.00 1721614.00 1884084.00 4012792.00 4233964.00 534639.00 1437115.00 H 1818369.00 827157.00 498597.00 1839170.00 6146388.00 3424262.00 4478509.00 2945072.00 6851837.00 3401657.00 1655989.00 4489112.00