Load time: 11/17/2010 10:55:14 AM Read time: 11/17/2010 10:55:14 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1159 Comment: 224, std. Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 92409952 cps Min counts: 63657 counts Well list: r0(a1:h9,a10:d10) = cps(f2(a1:h9,a10:d10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17038632.00 19041194.00 16554540.00 18197444.00 16800414.00 13423770.00 92409952.00 74168184.00 134028.00 190982.00 B 17145072.00 19560342.00 15750978.00 18219066.00 15637143.00 15597563.00 77752256.00 36753548.00 128304.00 127315.00 C 18328644.00 20081424.00 17631510.00 17202548.00 16629573.00 4641522.00 19252846.00 32398740.00 130167.00 130985.00 D 16593332.00 14288963.00 14642512.00 15243507.00 12606504.00 8974545.00 15526087.00 22792132.00 135682.00 132012.00 E 16322049.00 17514712.00 16729617.00 15957271.00 16261293.00 9711784.00 14389458.00 17956064.00 132506.00 F 19425362.00 19586014.00 19218546.00 18844576.00 19102038.00 5618052.00 6695701.00 6890607.00 133894.00 G 19697408.00 20164814.00 17864638.00 16389017.00 19327994.00 12351465.00 2448353.00 9705396.00 134902.00 H 21928090.00 18248944.00 16725701.00 17898628.00 18023580.00 14963926.00 8805938.00 7444806.00 133971.00 Load time: 11/17/2010 10:57:14 AM Read time: 11/17/2010 10:57:14 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1160 Comment: 167, 164 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32145888 cps Min counts: 778914 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21365230.00 22014048.00 20817234.00 24759586.00 24903980.00 26381160.00 22031422.00 22339008.00 20663926.00 20269340.00 21249414.00 21488178.00 B 20988432.00 20640926.00 21214054.00 24043830.00 24573908.00 18947306.00 22288700.00 22217306.00 22058188.00 20263592.00 19439422.00 19968526.00 C 23986520.00 24223886.00 23554926.00 24964562.00 27056250.00 32145888.00 26032188.00 24878128.00 22842194.00 20864802.00 21915012.00 20988262.00 D 24655194.00 21745660.00 23380778.00 25324862.00 27972916.00 28276340.00 26068432.00 20929328.00 24211012.00 22455446.00 23420922.00 20808836.00 E 25087746.00 23614124.00 25449890.00 26878390.00 28242510.00 27812206.00 22796866.00 22417702.00 24753842.00 22806682.00 23607756.00 20674128.00 F 24110140.00 21948418.00 23650046.00 25490034.00 27071294.00 29674394.00 21423050.00 21806722.00 22496302.00 11343654.00 22057000.00 20065286.00 G 22537246.00 20711784.00 21800860.00 23031652.00 26688582.00 27372200.00 24250886.00 22476440.00 21246286.00 20128908.00 22332476.00 19188624.00 H 23449480.00 21053272.00 19925818.00 22785132.00 24763130.00 25021500.00 25228976.00 22370252.00 22285700.00 20405864.00 21114224.00 18166674.00 Load time: 11/17/2010 10:59:02 AM Read time: 11/17/2010 10:59:02 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1161 Comment: 178, 219 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40674584 cps Min counts: 778914 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34254852.00 28154716.00 35725912.00 37907056.00 38169344.00 40674584.00 39753304.00 37653264.00 36845692.00 33595756.00 32439350.00 33780212.00 B 26460382.00 26074802.00 25956544.00 26842268.00 29526250.00 31781650.00 35473072.00 29450892.00 29906480.00 24340768.00 22894158.00 24561046.00 C 27145828.00 27256686.00 25019088.00 26098302.00 32926156.00 30944906.00 37480956.00 27506074.00 28368410.00 22929880.00 22447746.00 24610166.00 D 29445434.00 25685464.00 29907054.00 28834044.00 28460766.00 29559642.00 38322776.00 28354572.00 30123690.00 25809810.00 24204090.00 24640454.00 E 27186474.00 27960766.00 30248746.00 29681974.00 28561478.00 30114374.00 37032608.00 29780250.00 29462368.00 26971278.00 24811956.00 24399782.00 F 25174910.00 24261614.00 25079830.00 26307472.00 28215704.00 31554278.00 31681598.00 28577718.00 26055700.00 24096492.00 23978324.00 26864578.00 G 22385490.00 24653010.00 23950528.00 26606806.00 29882960.00 26433710.00 31458702.00 16753403.00 25505142.00 23937792.00 28199566.00 25775904.00 H 24376796.00 26128328.00 22722582.00 26684286.00 28181506.00 25427874.00 29881930.00 27788098.00 26449150.00 26199870.00 26187364.00 26000990.00 Load time: 11/17/2010 11:01:55 AM Read time: 11/17/2010 11:01:55 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1162 Comment: 220, 221 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27124276 cps Min counts: 778914 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18306976.00 21011872.00 20578814.00 23943314.00 23606468.00 27124276.00 10234652.00 10475234.00 9140126.00 10143658.00 9573393.00 10669577.00 B 14780353.00 16003401.00 15622654.00 16409906.00 21920070.00 24963202.00 9846350.00 11412613.00 9647729.00 10175980.00 9017632.00 9357147.00 C 16635468.00 17501176.00 16981316.00 16780654.00 17595226.00 22861032.00 14172900.00 11956993.00 13316367.00 10579912.00 10397723.00 10120726.00 D 16997212.00 17701068.00 15488052.00 19524694.00 18890234.00 10239468.00 14945895.00 15582493.00 13663509.00 11656589.00 11575586.00 11486666.00 E 15724364.00 16948762.00 17370816.00 17166154.00 20729864.00 23194676.00 10512444.00 12899199.00 12491568.00 11748791.00 11082715.00 11417281.00 F 15570874.00 16074506.00 15453974.00 17753842.00 18940764.00 20860832.00 10259010.00 11569208.00 12805046.00 10619145.00 9345740.00 10317196.00 G 11853907.00 14846785.00 13993670.00 16643965.00 18138708.00 20000110.00 10055528.00 10248575.00 9341392.00 9400219.00 8805600.00 9598426.00 H 17558814.00 18736512.00 15468152.00 20417846.00 20765232.00 19099088.00 9805854.00 9435565.00 9256568.00 10152702.00 8997550.00 8541354.00 Load time: 11/17/2010 11:03:47 AM Read time: 11/17/2010 11:03:47 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1163 Comment: 196, 179 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35098720 cps Min counts: 778914 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11744716.00 12443515.00 11944523.00 13362284.00 12334020.00 12594986.00 32791280.00 31887562.00 31377718.00 29042072.00 28059984.00 28723570.00 B 9423428.00 11057777.00 10671477.00 11273157.00 9114042.00 9878217.00 27980956.00 27156146.00 27516666.00 20933264.00 17457948.00 21563254.00 C 12139504.00 10627186.00 12617470.00 13051491.00 9972745.00 12411790.00 35098720.00 31366476.00 25047328.00 21944462.00 18948874.00 24420132.00 D 14943572.00 11402589.00 13203033.00 14013912.00 13019421.00 11838899.00 33702016.00 29985342.00 27313556.00 24932676.00 21206026.00 22772876.00 E 11122628.00 11014496.00 11530382.00 10720017.00 10617740.00 11079546.00 27498792.00 29000986.00 25878064.00 24423348.00 22045890.00 21485156.00 F 7660561.00 7668525.00 10960127.00 9081177.00 9379390.00 11231236.00 25811692.00 26393318.00 24085836.00 20967866.00 21179558.00 23021984.00 G 7641546.00 7778820.00 7280184.00 6936111.00 9048484.00 8702681.00 26112738.00 25837806.00 25177596.00 20182576.00 22301082.00 22021888.00 H 7676831.00 7027838.00 5691750.00 7717875.00 8062077.00 7367473.00 26978470.00 24443430.00 23558558.00 24383972.00 23029608.00 23247078.00 Load time: 11/17/2010 11:05:47 AM Read time: 11/17/2010 11:05:47 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1164 Comment: 222, 223 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 49305860 cps Min counts: 778914 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40014088.00 40175276.00 40263536.00 46712620.00 46644088.00 47343500.00 37850508.00 41749396.00 40174244.00 38101756.00 37060800.00 36024320.00 B 35317860.00 35732304.00 35590260.00 35433540.00 36215436.00 44590668.00 36587292.00 33473040.00 32444486.00 29049548.00 28281168.00 30886868.00 C 34529560.00 31202752.00 34952872.00 39259196.00 41787476.00 43941164.00 33607064.00 31004400.00 31031578.00 26436404.00 49305860.00 29504326.00 D 39829120.00 38537980.00 38781284.00 35358908.00 40344036.00 47623416.00 34086512.00 31504380.00 18464292.00 24437754.00 27696994.00 25839092.00 E 39580188.00 40028280.00 36384092.00 39163264.00 42052556.00 45893536.00 35557124.00 32023772.00 29601094.00 29606044.00 26760468.00 25303144.00 F 33242328.00 32478582.00 33280396.00 36420668.00 42718388.00 42439088.00 31834258.00 28427424.00 30946752.00 26676424.00 26115016.00 48268840.00 G 24865736.00 33513940.00 32786866.00 32184678.00 37831568.00 33560152.00 34352900.00 32835098.00 29737386.00 28334974.00 28077482.00 26442596.00 H 35539272.00 35233828.00 29529942.00 36356916.00 38864488.00 35889868.00 32004038.00 29041424.00 20181112.00 23402204.00 26868748.00 24650514.00 Load time: 11/17/2010 11:20:19 AM Read time: 11/17/2010 11:20:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1165 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33303870 cps Min counts: 931 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 31160434.00 25738934.00 25677844.00 175065.00 B 33303870.00 18774898.00 19505040.00 169360.00 C 16355670.00 18243772.00 14446864.00 1863.00 D 10261901.00 11130208.00 12694915.00 176149.00 E 4126691.00 4050770.00 6574736.00 180523.00 F 2706429.00 2729027.00 3552557.00 22756440.00 G 1694948.00 1547978.00 2058191.00 27649998.00 H 1131023.00 1166793.00 1216007.00 31328878.00