Load time: 11/18/2010 10:02:55 AM Read time: 11/18/2010 10:02:55 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1172 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 63617728 cps Min counts: 73585 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33568360.00 45623532.00 29578660.00 157042.00 B 36332520.00 63617728.00 40395904.00 147171.00 C 9516207.00 10797889.00 9450681.00 149149.00 D 7157435.00 6120406.00 6463164.00 156471.00 E 3821505.00 6606212.00 3620932.00 179257.00 F 2098998.00 2377784.00 2008193.00 55322620.00 G 3725722.00 1336068.00 1573810.00 58287576.00 H 846017.00 827092.00 766858.00 56400432.00 Load time: 11/18/2010 10:05:14 AM Read time: 11/18/2010 10:05:14 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1173 Comment: 174, 131 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37811160 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18028242.00 20074600.00 16358088.00 25201408.00 24130034.00 23337416.00 6575766.00 6730359.00 6242660.00 6608257.00 6708358.00 6351001.00 B 21128614.00 20354160.00 21435634.00 23100950.00 24753304.00 26683894.00 7174989.00 6686576.00 6408723.00 6575843.00 6418407.00 6247005.00 C 20243562.00 23246120.00 25314748.00 25589992.00 31901482.00 25809146.00 7182855.00 6900699.00 6617789.00 6681141.00 7030197.00 6670454.00 D 22207586.00 25353304.00 27638638.00 31624086.00 28212982.00 32282740.00 7257811.00 7729671.00 6972586.00 7209998.00 7513585.00 6860554.00 E 27737244.00 31528228.00 31730226.00 32972206.00 37811160.00 33799784.00 7940366.00 8960177.00 9097938.00 9136724.00 8924253.00 8243757.00 F 22854664.00 24814486.00 22680930.00 28921662.00 32021856.00 36480024.00 7716119.00 8331213.00 8429119.00 8550191.00 8477634.00 7705685.00 G 20807620.00 20964686.00 21752740.00 23198632.00 29147170.00 26112622.00 7473429.00 7998544.00 8645794.00 7531722.00 7641117.00 7694550.00 H 25029750.00 26128040.00 21772258.00 27197004.00 30699276.00 26989990.00 8752715.00 8864753.00 8190196.00 8061590.00 8322447.00 8591444.00 Load time: 11/18/2010 10:07:00 AM Read time: 11/18/2010 10:07:00 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1174 Comment: 132, 133 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42666292 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 30950774.00 30299754.00 30169274.00 34788108.00 37033048.00 35264472.00 25501616.00 25113344.00 23690706.00 23542508.00 23875854.00 22396438.00 B 30924604.00 20030160.00 30002888.00 31803156.00 35773272.00 39641104.00 25932172.00 25336978.00 24034298.00 21351388.00 22482272.00 19863106.00 C 32046886.00 35089748.00 31136950.00 34355320.00 36482932.00 42666292.00 27273846.00 24934896.00 25005006.00 23985978.00 21349106.00 22845816.00 D 33735232.00 34611292.00 35926200.00 33581528.00 39745056.00 38758336.00 27157468.00 29531680.00 27429798.00 26462098.00 25495772.00 22769296.00 E 28997338.00 30617592.00 31995686.00 32867732.00 32254006.00 34172524.00 22756324.00 23708442.00 23015298.00 24364742.00 22431326.00 20184816.00 F 29398948.00 27567144.00 28369870.00 30479952.00 32210528.00 37762408.00 21327246.00 22700692.00 21239794.00 20105998.00 19083560.00 20420530.00 G 27090512.00 24972784.00 26049492.00 28453492.00 31348578.00 31486810.00 20536104.00 20410416.00 19245788.00 18101002.00 19233478.00 18904114.00 H 27010390.00 26263514.00 23297638.00 28819802.00 31901742.00 31637700.00 18171302.00 20463978.00 17572020.00 19711126.00 17745618.00 17146924.00 Load time: 11/18/2010 10:08:41 AM Read time: 11/18/2010 10:08:41 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1175 Comment: 197, 198 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 39280920 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26854432.00 25685500.00 24216220.00 27744156.00 28484182.00 29053250.00 35551576.00 31469512.00 30737068.00 29077218.00 28216764.00 28129346.00 B 27271076.00 25006612.00 22564322.00 25765952.00 25228586.00 24200120.00 35635976.00 32281402.00 29670250.00 27426806.00 27382268.00 28324278.00 C 27745444.00 27356306.00 24612610.00 24124356.00 28337674.00 30389822.00 39280920.00 31165604.00 30401208.00 26751796.00 26669552.00 29397174.00 D 28135946.00 26646474.00 26911764.00 28399964.00 30388046.00 29703848.00 36064580.00 34533196.00 32691872.00 30977358.00 28890022.00 28814474.00 E 28395204.00 26439736.00 26000548.00 24040082.00 29224912.00 28893232.00 37548184.00 34107592.00 30546880.00 32469498.00 29019266.00 28166060.00 F 25929410.00 23779146.00 23063198.00 25580158.00 29004682.00 29718692.00 37022844.00 32938048.00 31242218.00 28934228.00 30341176.00 30606406.00 G 25189350.00 21456238.00 24428306.00 25890716.00 29144770.00 28552772.00 33692196.00 34844120.00 31208550.00 28290234.00 29293924.00 28487724.00 H 25521162.00 24913284.00 24005414.00 26195162.00 25976878.00 28511286.00 34681096.00 33378504.00 31021024.00 31982814.00 30589460.00 29548146.00 Load time: 11/18/2010 10:10:22 AM Read time: 11/18/2010 10:10:22 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1176 Comment: 199, 200 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41255020 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29770594.00 31088864.00 30168806.00 34738776.00 34500928.00 35718160.00 31058002.00 29715182.00 29137360.00 26149524.00 26904512.00 26969154.00 B 33261504.00 35441272.00 34119388.00 36462096.00 39186868.00 40940652.00 37029352.00 35393448.00 33020978.00 28626110.00 29297122.00 30958400.00 C 35019236.00 37431272.00 32550088.00 33178916.00 39852004.00 41255020.00 35554008.00 31218430.00 31632434.00 25513810.00 26673754.00 29094266.00 D 34837268.00 35540712.00 36230456.00 37581152.00 39008888.00 40165968.00 29415822.00 33594996.00 33669192.00 28381034.00 27655810.00 27302422.00 E 35477904.00 36466536.00 35985948.00 36806120.00 38157680.00 38393692.00 34683720.00 31293548.00 31577814.00 30025204.00 26896990.00 27260196.00 F 32764058.00 31312666.00 30577936.00 32323998.00 33122486.00 40737696.00 32549810.00 29752632.00 29490206.00 26249354.00 24855818.00 27724312.00 G 21760336.00 28644322.00 30014792.00 34350172.00 36004080.00 36223552.00 31374966.00 29691960.00 28318410.00 25379072.00 30807812.00 25602690.00 H 33673704.00 31591388.00 31255128.00 33979204.00 36104696.00 35900376.00 32413570.00 29692870.00 28533318.00 27468098.00 27545700.00 24118308.00 Load time: 11/18/2010 10:12:01 AM Read time: 11/18/2010 10:12:01 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1177 Comment: 134, 201 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46651912 cps Min counts: 779031 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24660760.00 26430228.00 25334012.00 27532170.00 30893086.00 29697282.00 44236736.00 44244524.00 42897000.00 45250672.00 43753004.00 46651912.00 B 23184618.00 21031528.00 16319330.00 26963366.00 23951760.00 23105538.00 41093984.00 43075132.00 41143248.00 37508772.00 41777144.00 44276204.00 C 21070152.00 20730432.00 18837784.00 19422168.00 21471850.00 22342932.00 37914952.00 40821800.00 37833568.00 35597548.00 38195092.00 46307772.00 D 19788282.00 20183718.00 19378928.00 21302228.00 23132022.00 21730352.00 36636832.00 37825300.00 41599124.00 37733152.00 38002992.00 41428120.00 E 20862672.00 20581080.00 20948450.00 21296348.00 19449712.00 20966154.00 39629008.00 36439928.00 41767516.00 36759920.00 40456324.00 41034672.00 F 18015524.00 16987044.00 16340588.00 16923022.00 20369964.00 24384344.00 40297488.00 35269104.00 36636144.00 35711448.00 36473196.00 40624676.00 G 14924851.00 16087605.00 14739831.00 18213574.00 19534524.00 20580756.00 33241066.00 35546220.00 34463380.00 38682320.00 36693432.00 35047124.00 H 16521135.00 18561836.00 13994837.00 18522514.00 18748248.00 18215106.00 35728644.00 35294828.00 33663804.00 35104288.00 30640834.00 36627360.00 Load time: 11/18/2010 10:13:55 AM Read time: 11/18/2010 10:13:55 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1178 Comment: standard retest Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 67079772 cps Min counts: 74080 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37912000.00 48495452.00 31183190.00 158183.00 B 49062616.00 67079772.00 42651108.00 148160.00 C 10465684.00 11204919.00 9581199.00 150062.00 D 7246658.00 6506716.00 6593888.00 158259.00 E 3856001.00 6696595.00 3801697.00 179732.00 F 3127902.00 2490162.00 1922354.00 57515560.00 G 1945332.00 1441454.00 1618768.00 59903340.00 H 881316.00 966561.00 740231.00 61818044.00 Load time: 11/18/2010 10:15:05 AM Read time: 11/18/2010 10:15:05 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1179 Comment: standard retest reverse Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59918544 cps Min counts: 76238 counts Well list: r0(a9:h12) = cps(f2(a9:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51799340.00 693387.00 935883.00 909370.00 B 47276464.00 1237434.00 1451040.00 1615305.00 C 59918544.00 1949093.00 2393411.00 2084836.00 D 155597.00 4409543.00 5256790.00 6184160.00 E 156319.00 6085732.00 5655918.00 7298117.00 F 152477.00 10664981.00 10622720.00 11607354.00 G 152763.00 39571036.00 59441240.00 33105178.00 H 153961.00 31874724.00 48504816.00 34014364.00 Load time: 11/18/2010 10:16:25 AM Read time: 11/18/2010 10:16:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1180 Comment: standard sensitivity 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 65410684 cps Min counts: 75242 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37070760.00 47946920.00 29428048.00 155695.00 B 48474172.00 65410684.00 41860312.00 150574.00 C 11970537.00 11178906.00 9644883.00 150484.00 D 6969004.00 5999704.00 6198523.00 155874.00 E 3926073.00 6678996.00 3777654.00 177436.00 F 2098068.00 2383584.00 1999152.00 56208304.00 G 2461207.00 1330103.00 1588846.00 56939204.00 H 845318.00 784495.00 730770.00 59530588.00