Load time: 11/30/2010 01:49:28 PM Read time: 11/30/2010 01:49:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1201 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56450912 cps Min counts: 75260 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 55593236.00 55044608.00 56450912.00 152973.00 B 36727732.00 35384332.00 35822568.00 150521.00 C 22629056.00 21365012.00 21680236.00 154798.00 D 13532607.00 13017856.00 12768124.00 154779.00 E 9660178.00 8993667.00 8959176.00 151566.00 F 5243056.00 5225019.00 5013751.00 158049.00 G 2712372.00 2569027.00 2605031.00 156338.00 H 2107802.00 2077320.00 2046662.00 155501.00 Load time: 11/30/2010 01:50:54 PM Read time: 11/30/2010 01:50:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1202 Comment: tsl plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2726902 cps Min counts: 337380 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 757035.00 983197.00 1853181.00 1101021.00 1168583.00 885467.00 970366.00 1197535.00 1348722.00 750743.00 1199988.00 1223503.00 B 775057.00 1112428.00 983406.00 931376.00 1296045.00 1005572.00 876038.00 1083836.00 1362447.00 1200634.00 1257398.00 1139023.00 C 674760.00 1816464.00 1081251.00 771312.00 1459950.00 1023423.00 1132122.00 1214074.00 1463542.00 1223712.00 1168260.00 1466793.00 D 920084.00 2353272.00 765590.00 941432.00 949207.00 1053953.00 2726902.00 1137122.00 1346175.00 1292072.00 1094292.00 992512.00 E 769373.00 1111363.00 895789.00 1122921.00 1751220.00 931642.00 958351.00 1116971.00 1322108.00 1173868.00 1013842.00 846496.00 F 755952.00 2102930.00 954967.00 1077392.00 1278613.00 1133624.00 1100280.00 957800.00 1042224.00 1112218.00 1180674.00 1249148.00 G 1257512.00 1069256.00 1027852.00 1465481.00 975993.00 1374461.00 1248976.00 884745.00 987132.00 1094539.00 1097543.00 878338.00 H 976943.00 1047717.00 1188544.00 1192897.00 1444190.00 1062754.00 1087695.00 1154592.00 1048687.00 1050398.00 1025894.00 1003044.00 Load time: 11/30/2010 01:53:16 PM Read time: 11/30/2010 01:53:16 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1203 Comment: tsl plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2172247 cps Min counts: 360296 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 968484.00 994128.00 980783.00 1160903.00 1206204.00 925730.00 1249109.00 845375.00 935501.00 954701.00 857560.00 1206736.00 B 752283.00 1442080.00 1179324.00 1033802.00 1215956.00 1079578.00 1083437.00 1034068.00 1356744.00 720593.00 1935438.00 1095452.00 C 981239.00 962191.00 948447.00 1304429.00 2172247.00 1010572.00 1005876.00 1092448.00 1325796.00 903013.00 1194570.00 1139346.00 D 772262.00 858454.00 969776.00 1110070.00 1072678.00 1108816.00 1252969.00 1087619.00 1015990.00 1149459.00 914077.00 1437385.00 E 828950.00 961013.00 1257835.00 967362.00 1273613.00 942668.00 1012682.00 878167.00 929836.00 988064.00 973046.00 1191376.00 F 882273.00 960861.00 1215063.00 1451015.00 855640.00 1201337.00 1110793.00 902063.00 1002778.00 1092030.00 1189095.00 1553194.00 G 1165560.00 1112523.00 997227.00 1142786.00 1108454.00 1502627.00 1030817.00 1209474.00 1007929.00 994090.00 1196395.00 1300398.00 H 940596.00 859613.00 1155846.00 1184381.00 1240954.00 1167880.00 986581.00 985250.00 1011560.00 913583.00 1416055.00 1380659.00 Load time: 11/30/2010 01:55:45 PM Read time: 11/30/2010 01:55:45 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1204 Comment: tsl plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 1791846 cps Min counts: 359289 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 927384.00 986106.00 1041824.00 928639.00 1065264.00 806157.00 1118416.00 1085528.00 1045645.00 1044334.00 990592.00 968522.00 B 963712.00 796101.00 1217914.00 1074331.00 1122978.00 928848.00 1013100.00 757682.00 1049162.00 1016636.00 1160599.00 1028612.00 C 983616.00 1086403.00 1234472.00 1033061.00 1003196.00 1048383.00 1155352.00 1192517.00 1038612.00 971658.00 1080814.00 1109253.00 D 1080871.00 940292.00 1113777.00 1255326.00 828798.00 915845.00 1182765.00 1217914.00 941889.00 986885.00 1055188.00 910180.00 E 1205196.00 985536.00 1154383.00 897405.00 1103417.00 1216089.00 900941.00 1086935.00 1244946.00 1014773.00 1072411.00 928810.00 F 1019659.00 887463.00 1041064.00 1074959.00 718578.00 1291996.00 1126172.00 1097410.00 1005819.00 1197383.00 967571.00 874270.00 G 821004.00 1009564.00 1087524.00 1081859.00 923981.00 1141684.00 1273081.00 1189342.00 947078.00 1206242.00 986372.00 988938.00 H 1430313.00 876684.00 1040113.00 924780.00 784790.00 1321557.00 968179.00 1791846.00 1054428.00 827030.00 872749.00 1049771.00 Load time: 11/30/2010 01:58:14 PM Read time: 11/30/2010 01:58:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1205 Comment: tsl plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 1476963 cps Min counts: 348596 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1181966.00 988938.00 1239167.00 799770.00 998691.00 1151892.00 1036311.00 919210.00 988444.00 951413.00 1022738.00 1017339.00 B 1131228.00 1039011.00 697192.00 963902.00 1096041.00 1044010.00 910921.00 1099520.00 885220.00 1011047.00 1243045.00 1155485.00 C 918240.00 1049428.00 1076404.00 1068400.00 1077373.00 1085471.00 1047185.00 962400.00 1125677.00 1127521.00 1225461.00 1203162.00 D 755952.00 901340.00 1064845.00 851743.00 1292833.00 940786.00 948371.00 1005971.00 757834.00 1180407.00 1047698.00 1122427.00 E 916168.00 921168.00 969263.00 903869.00 1226393.00 782889.00 1160884.00 873757.00 815263.00 1054656.00 1011998.00 1151531.00 F 875658.00 871552.00 1141285.00 881893.00 870924.00 747910.00 996523.00 1218732.00 896322.00 1279621.00 996580.00 994870.00 G 1001542.00 1102200.00 1190844.00 944797.00 942592.00 1034581.00 1056063.00 1143376.00 1027092.00 1154915.00 1111401.00 968845.00 H 1031806.00 956773.00 820890.00 769962.00 880030.00 726334.00 1044581.00 1040456.00 1179761.00 1476963.00 877939.00 849690.00