Load time: 01/04/2011 04:15:45 PM Read time: 01/04/2011 04:15:45 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1275 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 32259358 cps Min counts: 89 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 32259358.00 21250050.00 22483818.00 144273.00 B 18626900.00 12325748.00 9721129.00 143914.00 C 9128612.00 7097537.00 6278338.00 145351.00 D 5627130.00 3811848.00 4293270.00 148316.00 E 2416901.00 3168904.00 1906822.00 179.00 F 1218781.00 1446242.00 1924609.00 269.00 G 858725.00 910380.00 841118.00 269.00 H 545383.00 868338.00 547809.00 359.00 Load time: 01/04/2011 04:17:22 PM Read time: 01/04/2011 04:17:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1276 Comment: P#1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 107942920 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21209262.00 24166192.00 19628972.00 26337818.00 31536740.00 33632356.00 62578008.00 52994152.00 31626570.00 33574452.00 31008090.00 21928150.00 B 29992574.00 14648621.00 21525426.00 23380294.00 30127392.00 19275672.00 51918608.00 58938956.00 28431358.00 21534140.00 23538670.00 17053334.00 C 33996852.00 20387240.00 13864045.00 15638870.00 16493875.00 19333024.00 61878508.00 69388680.00 33493464.00 22512870.00 32707952.00 12323297.00 D 27306062.00 19904072.00 17287072.00 21806194.00 25512660.00 34148040.00 58953588.00 55484948.00 29829792.00 31984166.00 28278520.00 37271932.00 E 35336272.00 19453160.00 718.00 19058552.00 20566268.00 718.00 59980012.00 60875776.00 539.00 12814247.00 23245474.00 269.00 F 33578740.00 9916312.00 179.00 12219782.00 21412138.00 359.00 43897768.00 48161644.00 269.00 9565227.00 14624152.00 359.00 G 26835386.00 107942920.00 179.00 13731622.00 22676502.00 449.00 39514520.00 37755680.00 179.00 23174322.00 12891522.00 269.00 H 25041272.00 18327244.00 179.00 21695122.00 26753440.00 89.00 35254512.00 27289920.00 179.00 24695664.00 23484572.00 449.00 Load time: 01/04/2011 04:19:32 PM Read time: 01/04/2011 04:19:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1277 Comment: P#2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 140859872 cps Min counts: 89 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 47007632.00 34606108.00 49082964.00 34165224.00 45578452.00 35768320.00 25748136.00 21900066.00 10665137.00 101698992.00 46894584.00 50567116.00 B 52228560.00 33136968.00 88849464.00 38243280.00 45850204.00 34688776.00 21933852.00 17209568.00 54295992.00 92214832.00 52047052.00 45784256.00 C 65965564.00 31581074.00 109484560.00 42385640.00 46775896.00 43713424.00 24156402.00 19319504.00 75987200.00 94408336.00 54179424.00 30892890.00 D 40456416.00 37865636.00 131728984.00 45999112.00 48982532.00 49684072.00 26419362.00 16970892.00 59001520.00 85930280.00 115755112.00 32312446.00 E 60914980.00 37923508.00 269.00 122918424.00 47200712.00 628.00 28018446.00 18611254.00 449.00 76927184.00 34852896.00 179.00 F 56119880.00 42428452.00 359.00 37287948.00 60942316.00 359.00 26899068.00 24024046.00 539.00 56515076.00 34388380.00 449.00 G 58026972.00 38516336.00 628.00 140859872.00 59742788.00 359.00 22759130.00 17754462.00 628.00 32692260.00 26100130.00 449.00 H 53837132.00 41211108.00 539.00 96374120.00 74649872.00 628.00 13449031.00 21448910.00 269.00 18905716.00 19814842.00 269.00 Load time: 01/04/2011 04:21:43 PM Read time: 01/04/2011 04:21:43 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1278 Comment: P#3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 114601816 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 80867392.00 53823664.00 43764216.00 52573392.00 56960728.00 63580476.00 63880712.00 52715520.00 87926768.00 58255536.00 59133128.00 47981088.00 B 73565024.00 41190576.00 44906280.00 26293986.00 70073432.00 53049700.00 67430320.00 101725696.00 12668097.00 85702920.00 58664564.00 41258100.00 C 72808680.00 38195008.00 40690796.00 36160244.00 68922336.00 61848664.00 65821724.00 103155888.00 54786944.00 93381448.00 82544528.00 51338388.00 D 68703952.00 39733540.00 40882808.00 30418514.00 65004376.00 52587300.00 55111320.00 89116944.00 53129284.00 103724384.00 66845280.00 55208228.00 E 53095708.00 39176152.00 449.00 55250840.00 60184468.00 179.00 62183024.00 69755544.00 988.00 92343512.00 38661904.00 359.00 F 65765960.00 44676248.00 718.00 51505536.00 64161076.00 718.00 60204552.00 114601816.00 269.00 80465784.00 52712348.00 89.00 G 60936360.00 45521500.00 269.00 53744108.00 57094016.00 269.00 50013752.00 51922712.00 628.00 90949616.00 51892616.00 359.00 H 63689316.00 49724216.00 808.00 58200348.00 56453260.00 449.00 52484460.00 52157784.00 20572.00 67491792.00 52697400.00 89.00 Load time: 01/04/2011 04:25:13 PM Read time: 01/04/2011 04:25:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1279 Comment: P#4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 105859472 cps Min counts: 134 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 77612736.00 72160568.00 79229760.00 95705096.00 85647464.00 87556432.00 83749176.00 78767248.00 75026904.00 63148492.00 67715488.00 62177556.00 B 69071384.00 73740456.00 76781440.00 92348136.00 76032416.00 80233840.00 81686752.00 72797872.00 67755264.00 59075024.00 60737008.00 64619780.00 C 73274232.00 61565740.00 72620584.00 85834064.00 72954472.00 80950296.00 76900336.00 71533920.00 62555988.00 60973828.00 61989428.00 64467396.00 D 105859472.00 73484656.00 75799208.00 91676312.00 71876128.00 90371512.00 81218672.00 69499816.00 65726368.00 64040252.00 62595784.00 79508640.00 E 78325448.00 68329248.00 449.00 84171792.00 73849376.00 449.00 78436144.00 104741528.00 449.00 61949044.00 65771596.00 269.00 F 79028616.00 66176860.00 539.00 73069352.00 71109800.00 539.00 71662096.00 79488024.00 269.00 64021196.00 65269816.00 449.00 G 78406720.00 65255576.00 269.00 80360880.00 72339176.00 539.00 79076008.00 83378912.00 359.00 63089484.00 59459732.00 449.00 H 83778536.00 75715136.00 359.00 88905504.00 85756048.00 449.00 78999272.00 81351736.00 539.00 72079296.00 68737456.00 269.00 Load time: 01/04/2011 04:27:25 PM Read time: 01/04/2011 04:27:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1280 Comment: P#5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 44428704 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25301132.00 30979396.00 28855944.00 34115400.00 40957572.00 38331568.00 17054552.00 16055744.00 13559632.00 23147324.00 32557004.00 31504848.00 B 29808742.00 24897436.00 26857952.00 27565568.00 33249738.00 29456242.00 15467490.00 11226858.00 10537942.00 19780722.00 29023464.00 26868976.00 C 37000700.00 19693372.00 18350084.00 20883274.00 25638466.00 22795888.00 13035622.00 11435300.00 7698370.00 20203350.00 32589964.00 31633310.00 D 32108222.00 19899860.00 17167380.00 23566818.00 24991966.00 44428704.00 11590990.00 6905831.00 6424050.00 20838818.00 33266390.00 31918496.00 E 21408776.00 14158431.00 359.00 18120822.00 25492192.00 89.00 15682127.00 9257619.00 539.00 32550120.00 32303850.00 449.00 F 22825172.00 15195401.00 449.00 11329998.00 22247754.00 179.00 12123911.00 9940252.00 269.00 22210302.00 30604826.00 89.00 G 29939400.00 20683982.00 628.00 8883993.00 26018996.00 718.00 16213879.00 9358873.00 179.00 29737532.00 29211800.00 179.00 H 26067938.00 28122816.00 718.00 27626414.00 31141930.00 539.00 16986844.00 12199750.00 628.00 23600222.00 24059170.00 539.00 Load time: 01/04/2011 04:29:34 PM Read time: 01/04/2011 04:29:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1281 Comment: P#6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 99494144 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27751302.00 16276968.00 23754376.00 23740396.00 23886744.00 22942254.00 40120508.00 39807472.00 36802228.00 18275748.00 11867426.00 33246134.00 B 23327660.00 20961972.00 28450636.00 14216185.00 29667304.00 26020910.00 24781018.00 33110432.00 38888556.00 36097344.00 31219240.00 31602312.00 C 28772886.00 25840062.00 13910257.00 24238894.00 25002070.00 24642574.00 76429136.00 44464196.00 45548324.00 34152668.00 33932052.00 36514196.00 D 17884446.00 17934096.00 27240404.00 22765384.00 58313144.00 18529684.00 69685536.00 99494144.00 53496576.00 38112800.00 30063834.00 22503470.00 E 28227082.00 24498148.00 179.00 15587415.00 56112348.00 89.00 55019932.00 85152744.00 718.00 41911552.00 33792816.00 359.00 F 31036858.00 23673998.00 359.00 21945858.00 24817504.00 359.00 52798500.00 74834944.00 179.00 26765448.00 23167626.00 628.00 G 14383675.00 48321088.00 269.00 26092856.00 29039564.00 269.00 2281072.00 37671764.00 359.00 15170636.00 26132236.00 539.00 H 31864814.00 24400480.00 449.00 22038592.00 24224470.00 359.00 38101516.00 31326004.00 89.00 34728360.00 13324429.00 269.00 Load time: 01/04/2011 04:31:34 PM Read time: 01/04/2011 04:31:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1282 Comment: P#7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 106959560 cps Min counts: 0 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56440696.00 53900460.00 53597096.00 57787116.00 51508560.00 56224236.00 31545336.00 12846887.00 27977882.00 16460465.00 22395390.00 17286618.00 B 56133408.00 64764676.00 59550572.00 48770884.00 106959560.00 35135508.00 12159493.00 12096737.00 33405344.00 16232140.00 21803402.00 13633874.00 C 62052360.00 51306172.00 58644092.00 50290456.00 88701472.00 60922340.00 14440571.00 14346248.00 14454003.00 16668601.00 20417470.00 16489877.00 D 70414568.00 62334076.00 52700876.00 52785320.00 102070528.00 84133296.00 12511631.00 13286623.00 30399366.00 32515832.00 13988154.00 16594540.00 E 58795804.00 57931320.00 628.00 52755280.00 54062020.00 269.00 10729744.00 15621734.00 539.00 16542660.00 12539452.00 449.00 F 57550860.00 58790540.00 89.00 43413816.00 61873416.00 0.00 7663412.00 32476602.00 718.00 16970322.00 16424405.00 449.00 G 58175408.00 52736148.00 449.00 51814428.00 56291500.00 269.00 33564376.00 17701454.00 449.00 31815774.00 18374862.00 449.00 H 54111544.00 50794816.00 269.00 53593496.00 52004684.00 628.00 32126582.00 18169334.00 269.00 33453604.00 16881448.00 0.00 Load time: 01/04/2011 04:33:20 PM Read time: 01/04/2011 04:33:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1283 Comment: P#7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 108908728 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57080368.00 54799508.00 53825636.00 58410248.00 51752268.00 56524804.00 31330778.00 12896077.00 28058526.00 16362667.00 22333298.00 17084358.00 B 56625880.00 65921952.00 60173340.00 49220376.00 108908728.00 35555964.00 12223817.00 11890712.00 33565384.00 16228131.00 21744980.00 13629575.00 C 62873876.00 51827636.00 59302956.00 50521776.00 88849472.00 62081988.00 14464418.00 14378286.00 14382333.00 16669757.00 20387120.00 16327583.00 D 70993728.00 63442900.00 53486692.00 53423016.00 100997800.00 84705032.00 12449747.00 13069134.00 30161322.00 32472044.00 14050309.00 16380825.00 E 59154224.00 58696112.00 179.00 54319076.00 54472016.00 539.00 10737094.00 15822130.00 359.00 16900622.00 12515223.00 628.00 F 58136892.00 60031292.00 89.00 43754488.00 61951336.00 359.00 7641857.00 33542434.00 539.00 17224760.00 16310553.00 449.00 G 58941788.00 53564240.00 359.00 51880832.00 57082848.00 449.00 33232020.00 17758266.00 269.00 31460054.00 18415564.00 179.00 H 55003648.00 50645904.00 359.00 53231200.00 51868328.00 269.00 32161458.00 18158030.00 359.00 33055016.00 16798168.00 628.00 Load time: 01/04/2011 04:39:42 PM Read time: 01/04/2011 04:39:42 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1284 Comment: P#8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 194307040 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 152046944.00 167024288.00 78449528.00 2950068.00 322235.00 2256637.00 39436172.00 105317728.00 59691812.00 121820880.00 73667080.00 54058756.00 B 118969640.00 194307040.00 71723832.00 2736083.00 1192999.00 704390.00 61057400.00 107986624.00 105489528.00 99799088.00 73387488.00 56296408.00 C 176858512.00 83521440.00 131091680.00 127179192.00 120688912.00 1800818.00 78855864.00 69184232.00 13769163.00 102207184.00 112632792.00 50235804.00 D 107182320.00 158549296.00 66267788.00 155702000.00 134535936.00 1755721.00 69425456.00 98968840.00 69034576.00 110588192.00 84329008.00 27802298.00 E 74481704.00 73968144.00 3683.00 116471416.00 106418216.00 359.00 140507024.00 79641312.00 539.00 81323328.00 97541120.00 89.00 F 48905908.00 64503444.00 5479.00 5103665.00 101048816.00 179.00 108609760.00 93085912.00 269.00 92176496.00 48773600.00 449.00 G 67689696.00 53062780.00 5030.00 1652411.00 909662.00 449.00 106675512.00 61572128.00 269.00 90256248.00 39028300.00 449.00 H 56470068.00 48072792.00 6018.00 2613728.00 1060314.00 628.00 73363200.00 54477744.00 628.00 48443352.00 31844326.00 449.00 Load time: 01/04/2011 04:43:06 PM Read time: 01/04/2011 04:43:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1285 Comment: P#8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 199093824 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 156065872.00 171489328.00 80848024.00 2948900.00 316665.00 2230405.00 39269092.00 105044128.00 59837216.00 123628336.00 75336424.00 54770720.00 B 121816048.00 199093824.00 73763968.00 2741023.00 1207103.00 710589.00 62325424.00 110342128.00 106133368.00 100934904.00 75553456.00 56586524.00 C 181666176.00 86605384.00 135432208.00 129827120.00 123010064.00 1814922.00 80561984.00 70356808.00 14012978.00 102904896.00 115829824.00 50471052.00 D 109026192.00 163067936.00 67451824.00 159001472.00 137119872.00 1761291.00 70644112.00 100180832.00 68809304.00 110895488.00 84962608.00 27769022.00 E 75361736.00 75318848.00 3862.00 117105272.00 108916144.00 449.00 143332288.00 81476736.00 449.00 82736624.00 100546560.00 269.00 F 50403360.00 66359576.00 4761.00 5196913.00 103127272.00 449.00 110700976.00 94394288.00 898.00 93812376.00 49117224.00 539.00 G 69842136.00 55647480.00 4851.00 1676218.00 921340.00 718.00 112548736.00 62002696.00 988.00 90885136.00 39319360.00 539.00 H 58803412.00 50107944.00 5479.00 2671402.00 1088342.00 89.00 76083040.00 55424276.00 89.00 50041300.00 32620210.00 179.00 Load time: 01/04/2011 04:45:10 PM Read time: 01/04/2011 04:45:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1286 Comment: P#9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 394106208 cps Min counts: 134 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 90554104.00 93061672.00 259819232.00 91621064.00 82809984.00 107092184.00 12629975.00 11786545.00 9280092.00 11573963.00 11138259.00 11878475.00 B 115323040.00 103827112.00 248827536.00 101008880.00 117508056.00 104516912.00 16335730.00 11302702.00 10202163.00 11724887.00 11991875.00 13265524.00 C 96726512.00 155763952.00 394106208.00 91257272.00 134309568.00 103499736.00 17687372.00 10956302.00 12672210.00 12675131.00 13474250.00 17781916.00 D 121595448.00 144981904.00 390459456.00 101028856.00 183184640.00 106398528.00 18254298.00 13135513.00 14663752.00 14350036.00 14752982.00 18309014.00 E 76965320.00 110435520.00 144671248.00 102639112.00 107636640.00 449.00 7353840.00 11583458.00 269.00 13209448.00 12983298.00 15361.00 F 92644608.00 96736176.00 100276384.00 105165120.00 103236352.00 628.00 11475259.00 9313837.00 539.00 9751850.00 10522796.00 4222.00 G 73616680.00 86836336.00 90639320.00 121962608.00 85997760.00 628.00 9191938.00 5503069.00 718.00 7909532.00 7091518.00 18505.00 H 79830856.00 78438320.00 60837328.00 101867632.00 67674264.00 449.00 4888153.00 3134767.00 359.00 3057420.00 4699052.00 22458.00 Load time: 01/04/2011 04:47:05 PM Read time: 01/04/2011 04:47:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1287 Comment: P#10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 371355360 cps Min counts: 89 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 228925536.00 283522528.00 247450368.00 84974584.00 143133792.00 220069856.00 93486080.00 96101272.00 80100224.00 100417864.00 97197152.00 91745112.00 B 237627360.00 320465088.00 371355360.00 83035352.00 254477120.00 269205760.00 90669248.00 74895112.00 90459272.00 89883872.00 102986712.00 89276952.00 C 346124192.00 303345536.00 361742816.00 87531448.00 254115088.00 180636112.00 87837984.00 105056720.00 81476016.00 103325056.00 99075432.00 103429320.00 D 332040960.00 309411296.00 119419856.00 88155536.00 258026464.00 182718000.00 88650400.00 64339344.00 82049216.00 91493256.00 88771232.00 105018944.00 E 326736832.00 287406784.00 1167.00 149430096.00 212031264.00 449.00 82238088.00 80320176.00 808.00 85620360.00 130877184.00 449.00 F 330288480.00 286954080.00 449.00 110620112.00 158463936.00 359.00 92498576.00 148798640.00 269.00 47977396.00 83559368.00 269.00 G 266023600.00 171042912.00 988.00 109945504.00 131937744.00 539.00 86812984.00 109202848.00 808.00 107467808.00 43023416.00 449.00 H 148401968.00 125190936.00 179.00 107736224.00 121471008.00 718.00 85544920.00 81390952.00 179.00 79916680.00 80184184.00 449.00