Load time: 01/07/2011 11:02:19 AM Read time: 01/07/2011 11:02:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1296 Comment: Dario Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 75323480 cps Min counts: 874 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 75323480.00 69286184.00 68591192.00 136224.00 1920.00 4481187.00 9201455.00 2632319.00 11036512.00 10945869.00 8607831.00 5237124.00 B 57038020.00 49393712.00 48475148.00 136433.00 1920.00 5843668.00 5014450.00 5449427.00 12111984.00 10993564.00 9109429.00 5316903.00 C 41046420.00 37274760.00 24565838.00 138639.00 1958.00 5813099.00 6077184.00 5028052.00 12205425.00 11358809.00 9993531.00 7215239.00 D 17780280.00 16639532.00 17148554.00 135558.00 2034.00 4687919.00 5875673.00 6527997.00 13158106.00 12200224.00 9519349.00 4050661.00 E 10681503.00 10076172.00 10390200.00 137194.00 1920.00 3893639.00 5146478.00 5212585.00 13402598.00 12569940.00 10707317.00 7451365.00 F 6213694.00 5765817.00 5968435.00 136129.00 1863.00 5071795.00 4113808.00 4907226.00 10996575.00 11010783.00 10896345.00 10354494.00 G 3489456.00 3393173.00 3476845.00 137916.00 1882.00 3350230.00 4618046.00 1787.00 11449797.00 9291576.00 1749.00 6814470.00 H 1835019.00 1889443.00 1869172.00 137593.00 1825.00 3618917.00 3121536.00 1901.00 11352401.00 11180363.00 5780.00 13566954.00 Load time: 01/07/2011 11:04:29 AM Read time: 01/07/2011 11:04:29 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1297 Comment: standards helen Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37740212 cps Min counts: 846 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19784938.00 25320050.00 32074284.00 30136036.00 25729828.00 37740212.00 138905.00 1901.00 1844.00 1977.00 1787.00 1882.00 B 27041398.00 14525683.00 17243508.00 16922504.00 19572686.00 15039099.00 137460.00 2034.00 2167.00 1901.00 2015.00 1844.00 C 11541076.00 8998564.00 9110293.00 11464478.00 10948007.00 9293815.00 136718.00 2072.00 1882.00 1939.00 1806.00 1844.00 D 7166431.00 5203736.00 4769129.00 9836238.00 5147026.00 7565911.00 134551.00 1996.00 1958.00 2053.00 1939.00 1996.00 E 2771115.00 2759483.00 2835945.00 5412527.00 3004876.00 3054301.00 138297.00 2110.00 1958.00 1825.00 1768.00 1863.00 F 1952885.00 1353174.00 7318295.00 2293705.00 1546348.00 3213029.00 135501.00 1901.00 2091.00 1863.00 2034.00 1825.00 G 1613302.00 1664042.00 1804191.00 1184586.00 1607288.00 980268.00 137650.00 1958.00 1996.00 1863.00 1920.00 2015.00 H 725560.00 935468.00 644727.00 653683.00 784279.00 723830.00 136794.00 1977.00 1920.00 1882.00 1787.00 1692.00 Load time: 01/07/2011 11:06:44 AM Read time: 01/07/2011 11:06:44 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1298 Comment: wheat #1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14129413 cps Min counts: 893 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6014826.00 8871104.00 6791338.00 8849041.00 10938291.00 9941259.00 13354707.00 7773572.00 9596196.00 10975129.00 10692324.00 8375837.00 B 8552997.00 5917134.00 7317085.00 8095995.00 10125518.00 5704491.00 9107810.00 7167704.00 8467378.00 6600441.00 6782886.00 6612690.00 C 9313586.00 7099610.00 4698314.00 5599860.00 6030624.00 6652418.00 9455935.00 7384161.00 8722217.00 6107732.00 8380009.00 4633591.00 D 7897409.00 7704496.00 6067271.00 7426752.00 8919921.00 11397972.00 8569957.00 7545162.00 8085229.00 7729261.00 7557087.00 14129413.00 E 8594695.00 7324469.00 1977.00 7123265.00 7329604.00 1787.00 9273764.00 8331941.00 1825.00 3531017.00 7355069.00 1863.00 F 8525640.00 3471763.00 1977.00 4815413.00 7654665.00 2015.00 9856017.00 11862791.00 1939.00 3065542.00 4964916.00 1806.00 G 7122646.00 10835166.00 1825.00 5185965.00 8469366.00 2034.00 9397542.00 10005047.00 1825.00 7997239.00 5258456.00 1825.00 H 8510409.00 6491153.00 1863.00 8531010.00 9936212.00 2053.00 13372707.00 9775413.00 1863.00 9611104.00 9590287.00 1939.00 Load time: 01/07/2011 11:08:39 AM Read time: 01/07/2011 11:08:39 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1299 Comment: wheat #2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18726464 cps Min counts: 922 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9572301.00 11885909.00 11134921.00 13437167.00 13217143.00 12455560.00 14458164.00 17248546.00 3976063.00 5292825.00 14543965.00 6953025.00 B 10905454.00 12007473.00 11342598.00 12214574.00 11578599.00 11224140.00 13662506.00 13378357.00 6884261.00 6349684.00 7868432.00 13015163.00 C 11191930.00 11920054.00 5441804.00 12145622.00 12162368.00 12027609.00 14714154.00 15891532.00 5876507.00 6108872.00 6532333.00 14197330.00 D 7405631.00 12860920.00 5150460.00 12229745.00 12404835.00 13539173.00 14290351.00 14967137.00 6109471.00 6159737.00 18726464.00 18333490.00 E 10669927.00 12217754.00 2015.00 6172971.00 11126903.00 2167.00 13822807.00 16353706.00 2091.00 6544452.00 14573519.00 1939.00 F 11751037.00 12390922.00 1882.00 11776036.00 10525688.00 2053.00 13899574.00 14510528.00 2148.00 6203430.00 14754374.00 1882.00 G 11257283.00 10993144.00 1920.00 5840915.00 11331674.00 2015.00 11248602.00 12719340.00 2167.00 13942521.00 13729992.00 1844.00 H 10752802.00 12044703.00 1920.00 10830616.00 11297124.00 2053.00 7352493.00 12406020.00 2015.00 9460576.00 10946577.00 1882.00 Load time: 01/07/2011 11:10:33 AM Read time: 01/07/2011 11:10:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1300 Comment: wheat #3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23663556 cps Min counts: 922 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16768137.00 16064995.00 15267810.00 16462625.00 16352742.00 13688223.00 19320556.00 15670595.00 23663556.00 14876144.00 15457526.00 14243623.00 B 16759550.00 14579930.00 14038013.00 13697779.00 13296275.00 13277688.00 16706165.00 6522257.00 3218111.00 13935038.00 15890527.00 13382887.00 C 17137272.00 15093101.00 14570385.00 15169650.00 13723098.00 12945291.00 12660888.00 5944506.00 14599798.00 16112921.00 16030310.00 17466714.00 D 16974394.00 14758150.00 14462863.00 13402854.00 12933318.00 14031488.00 12785339.00 13448211.00 14831047.00 7335050.00 15699465.00 17596422.00 E 12419512.00 13186711.00 2053.00 15685585.00 13370710.00 1939.00 12687019.00 13466557.00 2167.00 19060410.00 10171495.00 2015.00 F 15072510.00 13268685.00 2072.00 14682089.00 12912412.00 2129.00 13391561.00 10781933.00 2015.00 19016804.00 15904220.00 1958.00 G 14909949.00 13758466.00 2072.00 15095792.00 15472018.00 2072.00 12774037.00 14585500.00 2034.00 22698616.00 14953373.00 1844.00 H 13323774.00 13726048.00 2053.00 15955111.00 14797600.00 2015.00 14580885.00 13935087.00 1863.00 20818784.00 17476626.00 2072.00 Load time: 01/07/2011 11:12:23 AM Read time: 01/07/2011 11:12:23 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1301 Comment: wheat #4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21541710 cps Min counts: 931 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19888000.00 17783610.00 19059106.00 18572586.00 19078386.00 19163588.00 21541710.00 18680650.00 18136556.00 15082930.00 15995740.00 15493748.00 B 16958612.00 16540516.00 17075544.00 18206806.00 17721984.00 18419558.00 20194194.00 16048543.00 16405961.00 14078766.00 14451269.00 15398818.00 C 18696256.00 15811491.00 17022770.00 16592490.00 16933356.00 18083372.00 17143722.00 14947403.00 15173195.00 14276887.00 13556069.00 15223941.00 D 10426574.00 17375842.00 16877142.00 17198196.00 16514424.00 19400974.00 16304850.00 15189501.00 15124638.00 14041759.00 14064123.00 17731504.00 E 18067390.00 15360522.00 2034.00 16397224.00 16312261.00 1977.00 16702048.00 8099986.00 2053.00 13582855.00 13568869.00 1863.00 F 19258354.00 14834268.00 1920.00 14134413.00 15673117.00 1863.00 16386735.00 16897316.00 1939.00 14976001.00 14673485.00 1901.00 G 18253916.00 15021579.00 1977.00 16958058.00 17231184.00 2281.00 17904658.00 17531544.00 1958.00 14920687.00 13284687.00 1939.00 H 18230244.00 16036845.00 1958.00 17716782.00 18196852.00 1901.00 18815812.00 17390544.00 2129.00 16133313.00 14990614.00 2015.00 Load time: 01/07/2011 11:14:10 AM Read time: 01/07/2011 11:14:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1302 Comment: wheat #5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23961678 cps Min counts: 903 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12703573.00 16999402.00 15436508.00 17852456.00 20011458.00 19986400.00 9160105.00 8358892.00 7502455.00 11241473.00 16074113.00 16258800.00 B 18268688.00 13116358.00 14625652.00 14795575.00 18239678.00 15209908.00 8648377.00 5993567.00 6029695.00 10722101.00 14034878.00 13607035.00 C 19033674.00 10569096.00 10056474.00 10994742.00 13327262.00 12930655.00 7537655.00 5892160.00 4258683.00 10377811.00 16853076.00 15302817.00 D 17461230.00 11040251.00 9548877.00 12267190.00 14134592.00 23961678.00 6804706.00 3723486.00 3517435.00 11118025.00 16718144.00 16482515.00 E 11747332.00 7872647.00 1863.00 10192781.00 13334655.00 1806.00 8151205.00 5035539.00 1920.00 17731140.00 16755475.00 1882.00 F 11531507.00 8489223.00 1806.00 6126690.00 11603369.00 1901.00 6582339.00 5451887.00 1977.00 12033520.00 15910523.00 1901.00 G 15760620.00 11049789.00 1939.00 4900336.00 14554619.00 2072.00 9006556.00 5352801.00 1939.00 15703200.00 15199846.00 1844.00 H 13193300.00 15725996.00 1958.00 14234305.00 16247742.00 1901.00 9122349.00 6545454.00 1977.00 11959589.00 12661835.00 1844.00