Load time: 1/19/2011 10:13 Read time: 1/19/2011 10:13 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1325 Comment: wheat Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45563736 cps Min counts: 65839 counts Well list: r0(a1:h7) = cps(f2(a1:h7)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 41417064 23970994 20219694 45563736 42387324 42464008 133808 B 19713778 13485676 15028259 29031432 31311876 30766838 131678 C 13364420 9052747 7433573 20210570 20700612 19487114 139402 D 10807316 5320372 5209245 12109119 11682879 11715881 139878 E 4946199 3687477 3189514 7390275 7371387 7374065 134056 F 4539202 1489230 1514554 5032926 5058299 5145819 133961 G 882781 1195014 1113488 24215380 3828570 3880760 137252 H 724961 628766 654908 2959611 2648051 2693256 148630 Load time: 1/19/2011 10:16 Read time: 1/19/2011 10:16 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1327 Comment: 425 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36780568 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27876324 22949054 20007942 25132204 28657774 29019102 28107780 31936802 29352144 24839336 26115494 35438808 B 24134462 19588702 19623778 24127664 24735738 30267580 31707838 28069408 26187368 23938226 22736404 26963884 C 28607906 28162528 21156090 25470964 29345842 31269158 35892236 34272540 31314772 26910718 27318822 28775336 D 29205948 25884840 25457232 27863668 29985970 30751780 36033132 35488028 33266854 31426530 33788268 31455452 E 27215402 27804512 26569862 29351258 28961516 32018544 36296280 35826896 30594954 35076240 30803076 32048170 F 28671478 23174604 22770020 23519984 28684354 30554648 34714368 35131428 32076664 26883888 31456340 30343948 G 26975736 22019022 21098184 26115494 25768436 31087790 33753584 33259330 36780568 29481170 27399792 32427300 H 26900406 22972800 23982722 24859780 29986546 30175226 33086210 35140772 33260610 31080474 32524880 31440222 Load time: 1/19/2011 10:18 Read time: 1/19/2011 10:18 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1328 Comment: 427 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36152320 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25738820 25043124 24324146 27471168 27239564 32642862 29190740 26302536 24750034 25408182 24255780 24621792 B 25029126 22471796 24145904 25231476 27895882 32204450 28092982 25390712 24641410 23935062 20532734 25671666 C 24351582 27133622 25409754 26260610 29445860 34237460 30240214 29426846 23917874 23724410 25138204 26241598 D 27160668 26891496 28929480 28213506 31464596 29391218 31754480 31431850 29087992 27633358 29392996 26947378 E 27030192 30072058 29038676 32182106 32254834 34728140 31549942 31291886 23747472 31023064 28102202 29284860 F 26338270 25741542 27282000 25759664 30048522 36152320 29292232 30949018 25178246 23684320 27667792 27581914 G 24036868 24336526 25176862 27692078 26814326 33378566 29414184 28424260 26153180 25022378 22763040 30069044 H 23831784 24497564 25924882 4801107 30541116 33062066 29519028 28982414 27973788 27230998 29608752 27323894 Load time: 1/19/2011 10:19 Read time: 1/19/2011 10:19 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1329 Comment: wheat 8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16115177 cps Min counts: 875 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6641407 5889216 13783737 2383664 303329 1262385 9852053 5070800 12132609 5224156 9565664 9049750 B 6905597 6694155 13735172 1210939 971232 488945 8536376 4502536 4566444 5030909 9609649 9717241 C 6659478 13234463 7125641 6310116 6713029 957838 9217252 10040241 2565874 5274407 4888470 7667590 D 7070226 7018768 16115177 6593425 6976599 1048249 7310967 3907339 11471131 4930183 7513180 5012806 E 14947061 15423118 1959 6047804 6858465 1826 4557951 8819524 2054 7452611 5132436 1864 F 11978422 14589606 1921 2903666 6958645 2092 4587763 9632014 2016 10106294 7844563 1864 G 12230672 13151001 1997 1557209 1185273 2207 5072440 10046712 2187 10709105 8471437 1959 H 13466449 12771652 1921 2181249 940924 2035 9801978 9136498 1769 11412653 8388113 1750 Load time: 1/19/2011 10:21 Read time: 1/19/2011 10:21 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1330 Comment: wheat 9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15574214 cps Min counts: 865 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12230520 12595124 13474156 13659601 11602690 13982803 9135351 7533706 6446894 6823002 7368983 8020833 B 13606417 12553875 13432237 13626355 13943973 13623448 9310959 7262768 6452259 5834013 7779030 7384484 C 13725640 13142552 11297190 11689117 13696500 14220221 11826489 7027293 8429989 6760286 8505667 8347716 D 13060789 12630787 10819515 13498709 12745271 14960461 11844842 9065880 9762182 11101194 9731656 8314371 E 11434942 15129794 2092 14580841 14276438 1940 5144179 9064403 1921 8786025 8361330 1864 F 13235114 13307049 2054 15574214 13688970 1997 6923973 5415178 2302 6440644 5922051 1826 G 12657540 13436798 1997 15385850 12575288 1997 10624280 3911038 1978 4369673 5300379 1769 H 13468497 13251430 2054 14553123 10127585 2149 2898289 2626002 1940 1814814 3386778 1731