Load time: 1/25/2011 11:13 Read time: 1/25/2011 11:13 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1341 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 85062696 cps Min counts: 61314 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37254636 39603240 40652836 122629 B 22648938 25355834 25265892 123504 C 15920071 15875701 16069253 123637 D 10053185 9640384 10931173 123827 E 5616055 5625774 5856275 124588 F 4364528 4005538 4445297 85002392 G 3272990 3113434 3335276 85062696 H 2218425 2118177 2158287 82273144 Load time: 1/25/2011 11:15 Read time: 1/25/2011 11:15 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1342 Comment: plate 422 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46698236 cps Min counts: 779225 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28334542 23240826 24227836 28193784 31690000 26615890 21484968 20975902 19740470 16553096 24138674 18538066 B 28231168 22800970 21185890 24427818 23094866 36006076 21844298 20502856 20399586 20651250 21430252 21175928 C 28013466 30014886 26940256 29542076 33003204 36090292 24495932 25099544 24375628 22503730 19789548 22553296 D 30157882 28632414 31018638 30611520 36055384 36625480 26594996 26181272 26628896 24649282 26013634 24459026 E 29277240 30655958 30746924 33848312 27170498 36621524 25699790 25338768 25998530 36066064 24738100 44516228 F 28258604 27524644 27150524 33850224 33944896 34586740 25159434 25525862 25021606 26558738 21899858 23602304 G 23616828 20036910 25233494 29672568 31313182 29737568 23569608 23006970 22925880 20876992 18147772 25944000 H 26496514 31333200 23269838 31973078 42810348 46698236 27267382 28066036 27417146 27092656 28505258 26841056 Load time: 1/25/2011 11:16 Read time: 1/25/2011 11:16 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1343 Comment: plate 423 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31071080 cps Min counts: 779225 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11060264 26806706 14465379 18152508 22093774 18237308 25615564 23462096 19371428 20091830 22262502 19866442 B 14650344 18241618 12451332 20517216 24585274 24996246 28036448 24065244 16045429 22431242 22063062 21491486 C 20542314 22344532 19086734 21088930 22958572 25573360 28983002 27664184 24929948 23132094 13506225 21579036 D 21099494 21459584 22027822 23384924 26381510 25281716 30304136 30164654 28183076 26779160 28604562 21727898 E 22302134 22491586 14442991 24402804 25090978 25386566 29988086 30319466 26325182 30006100 26394288 25792344 F 20552932 20064410 18048864 24784602 23628786 25383258 31071080 29089142 26251836 29867626 26260332 25621544 G 18237650 19122988 19299078 22177788 20178194 25962584 28703818 28967164 25216426 26485438 24633852 28079184 H 15043919 19908620 19873280 10505881 22425366 27750784 29342062 27560176 26405560 18257946 27145224 28395150 Load time: 1/25/2011 11:18 Read time: 1/25/2011 11:18 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1344 Comment: plate 424 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38571124 cps Min counts: 779225 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25071764 23559846 24088680 27955878 29393196 32294678 27274254 27070444 26565528 25319420 25399888 22703512 B 25215612 21531632 24273724 29182724 28936294 32459070 32415322 29103482 28852506 27579488 26556744 27005708 C 26504806 27091620 24050612 27836142 29793168 32805208 27973942 26616072 23788348 20587470 17800370 20986750 D 27702640 26098234 28936184 30619338 32527358 38571124 34097588 33572540 31837606 29374472 31731942 26925738 E 28033422 26552132 27330404 30460148 32178918 33143014 33478926 33064118 31000494 30916124 29845772 29823386 F 24627466 22484964 24516434 29634202 29548238 32027482 32319326 32967460 27867398 25203540 27446210 25513994 G 19507750 16706232 17007194 21447240 35443504 27001404 32592254 29770744 27412610 24181596 24102762 26563536 H 20542528 18791548 17320548 21107438 25885652 26297020 27602350 27113584 24533106 25043078 26227538 25075960 Load time: 1/25/2011 11:19 Read time: 1/25/2011 11:19 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1345 Comment: plate 426 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26180306 cps Min counts: 779225 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17025438 17823414 15882527 19100910 20500160 20222184 23369706 22350814 20484962 19201968 19239040 17434050 B 17385196 16783812 17125570 20924646 20277806 22278542 23042008 21763702 19701042 18032072 17898292 19646154 C 18329194 20527920 17250604 19932554 21046152 22054508 23630076 21994932 21011084 17670912 19083788 19168336 D 19174846 19985826 18743410 22332686 23141300 23776226 23420488 25560106 23151886 23185160 23070660 20565192 E 17191918 21208088 20836352 21718388 22831366 25143360 24685436 24700460 11579402 26180306 12993067 21208954 F 17430698 18549234 18382552 19041442 20675306 24703828 24029698 22961414 20521648 19484678 22104496 21800844 G 15471750 16822076 17347962 19809674 19796388 25315060 23294810 20741844 21620708 12300478 19465794 21820380 H 16728467 18444820 18041842 19852320 22744730 23537712 21696378 23234936 21347064 16730191 23166824 21562970 Load time: 1/25/2011 11:21 Read time: 1/25/2011 11:21 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1346 Comment: plate 428 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29234950 cps Min counts: 779225 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20547568 19583940 18920128 22965002 21861028 20091878 25778182 22334928 20808420 21226864 19638280 16848918 B 19718238 19244980 19591278 29234950 22558648 26528356 24322518 22973126 20737644 21884356 18961330 22594758 C 21563928 20670756 19181360 22819930 23622554 25064826 27027344 24417410 21690520 22076750 20903538 21497712 D 22427046 17029492 22778974 22653020 24855754 28105212 27561346 26084606 23836156 22180818 24937208 23728080 E 21178228 22848304 22999298 22445712 25742754 27359386 28208582 26985882 24731662 27544392 24290522 24474926 F 20810756 20267784 20123584 18921184 24143086 25072330 28273678 26077270 22645714 20669438 23705848 22790632 G 19248404 19075750 19060400 22747056 20472852 26639276 27178650 24399290 24334518 22907882 19253016 24107312 H 18648002 19344258 19294780 21183410 24890290 24139874 25561364 24529474 22422590 15493962 24932340 22147214 Load time: 1/25/2011 11:23 Read time: 1/25/2011 11:23 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1347 Comment: plate 429 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40454448 cps Min counts: 779225 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28197964 26339864 23565832 29126004 28354442 27545272 25397406 27205314 24290220 25835528 27434230 28194392 B 27229744 24479770 40454448 29823616 27223940 32739736 25282288 24282198 25110710 30261768 28672978 32026820 C 28796848 29237692 27174004 28989148 31017648 34236768 26889592 29649990 26125182 30059576 31751594 33132022 D 29251520 30278702 32764378 33560544 31538904 36316860 27918416 30395984 32340520 34199504 34868104 34862796 E 28192558 31538648 32143876 34746208 31665662 36479564 29085124 33177590 32262856 33996280 32747170 36000248 F 27804358 28126920 27961798 27749106 32108908 36183968 27693188 28291336 28430686 27285044 30383304 32683436 G 28666122 26606982 27297280 30362114 25262452 35611640 26474370 23542690 25076524 32470568 28941450 32583396 H 29511082 29098050 30724254 34511832 39345680 35640472 30910726 29561350 29564490 33083070 35605940 33713728