Load time: 01/27/2011 02:18:07 PM Read time: 01/27/2011 02:18:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1349 Comment: standards for atc4x Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 51104396 cps Min counts: 865 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49816392.00 51104396.00 202190.00 1978.00 1959.00 1978.00 2073.00 1959.00 2054.00 2054.00 1807.00 1883.00 B 29810394.00 29576720.00 155851.00 1788.00 2054.00 2111.00 2168.00 2073.00 1940.00 1978.00 1883.00 1807.00 C 18720930.00 19626824.00 144818.00 1959.00 1864.00 2092.00 2073.00 1978.00 2016.00 1902.00 1997.00 2016.00 D 12083066.00 12678226.00 1883.00 1978.00 1902.00 2092.00 1883.00 2073.00 1921.00 2035.00 1902.00 1864.00 E 6738545.00 7062121.00 1807.00 2016.00 2244.00 1750.00 1997.00 1978.00 1845.00 2035.00 1921.00 2016.00 F 4949305.00 4752189.00 1731.00 1921.00 1750.00 1883.00 2111.00 1959.00 2168.00 1788.00 1959.00 1826.00 G 3569805.00 3647223.00 1864.00 2035.00 3975.00 2035.00 2092.00 2054.00 1959.00 1959.00 2016.00 1750.00 H 2451266.00 2492209.00 2597999.00 1826.00 1940.00 1902.00 2073.00 1959.00 1959.00 1845.00 1883.00 1940.00 Load time: 01/27/2011 02:20:53 PM Read time: 01/27/2011 02:20:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1350 Comment: atc4x p429 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35396880 cps Min counts: 66540 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27005518.00 22772694.00 21263630.00 24664086.00 24643026.00 27488172.00 22091136.00 22658258.00 21875916.00 23103568.00 24288282.00 26764238.00 B 27819906.00 22104234.00 32906212.00 25600474.00 22828342.00 31145102.00 23411580.00 21734528.00 22231950.00 24439736.00 24657376.00 26672984.00 C 26355580.00 23090354.00 22305780.00 24611270.00 25802756.00 29463426.00 22828540.00 23234952.00 20027584.00 23716510.00 24626202.00 25739424.00 D 28730844.00 23480156.00 25075248.00 25860906.00 26224122.00 35396880.00 25490756.00 23711458.00 23160500.00 26468750.00 26495930.00 27593892.00 E 28338050.00 25451788.00 24105236.00 29467434.00 26501428.00 29998620.00 23577642.00 24571064.00 25981024.00 27583340.00 26482422.00 28545692.00 F 27178660.00 22634300.00 23273472.00 23859946.00 26211328.00 29023220.00 23498498.00 23028526.00 24274890.00 23054008.00 133081.00 28002170.00 G 22861026.00 21581856.00 21966946.00 24390160.00 21189726.00 29806174.00 21840840.00 19041320.00 21890234.00 26526240.00 22789212.00 28305212.00 H 23918908.00 20529092.00 21417350.00 24389018.00 26924982.00 29254346.00 20879632.00 20819438.00 23707778.00 22470332.00 25450706.00 23041598.00 Load time: 01/27/2011 02:22:41 PM Read time: 01/27/2011 02:22:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1351 Comment: standard w8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 106645568 cps Min counts: 1921 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18836472.00 16478159.00 16131645.00 44733476.00 134926.00 7513.00 19063868.00 16975046.00 17547772.00 18950688.00 18294982.00 18376404.00 B 12178135.00 10217368.00 10664572.00 43947276.00 132472.00 7437.00 17035954.00 21876590.00 18522046.00 16258681.00 16191984.00 17075192.00 C 6023784.00 7787800.00 5734994.00 36667712.00 129809.00 6391.00 16898362.00 18725294.00 17707370.00 17217024.00 17452654.00 17240222.00 D 3896587.00 3525753.00 3874481.00 39135640.00 691069.00 6600.00 17983400.00 19752252.00 17669262.00 19101116.00 16682794.00 20002650.00 E 2403804.00 2116630.00 2351822.00 94676984.00 135174.00 3842.00 15043620.00 17414660.00 9549.00 18199774.00 17579406.00 7913.00 F 1456213.00 1474988.00 1470784.00 4501205.00 132301.00 10538.00 17110814.00 18355882.00 7380.00 17171072.00 17493246.00 9682.00 G 1157578.00 1363745.00 922726.00 88806680.00 133481.00 5668.00 15445491.00 19410996.00 7304.00 19512592.00 16219152.00 9111.00 H 638986.00 605810.00 987403.00 106645568.00 158857.00 47137.00 21517426.00 20977390.00 52787.00 23230656.00 22534798.00 72475.00