Load time: 02/02/2011 02:21:03 PM Read time: 02/02/2011 02:21:03 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1353 Comment: standard, tsl enriched Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65983892 cps Min counts: 940 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37663340.00 35879456.00 29737988.00 179599.00 1937.00 3981849.00 3880447.00 4107455.00 2089.00 2013.00 1956.00 1880.00 B 38869748.00 12410889.00 15798023.00 182810.00 2146.00 1578080.00 2102813.00 4591492.00 1899.00 2070.00 2127.00 1880.00 C 65983892.00 17723592.00 9601394.00 193734.00 2032.00 1925707.00 2707970.00 15794399.00 1994.00 1994.00 1994.00 1880.00 D 13893112.00 6805374.00 5912365.00 186249.00 1918.00 1885188.00 7542824.00 3070938.00 1956.00 2032.00 1994.00 2089.00 E 5069603.00 4577224.00 5944294.00 185128.00 2070.00 2187489.00 6872001.00 8051743.00 2184.00 2089.00 1975.00 1918.00 F 5561306.00 3504640.00 1644702.00 184368.00 2013.00 2344665.00 3936089.00 8274550.00 2032.00 2146.00 2146.00 1880.00 G 1142327.00 1447834.00 955983.00 260916.00 2127.00 4761353.00 3832262.00 2241.00 2032.00 1956.00 2032.00 2013.00 H 780240.00 676523.00 636986.00 183437.00 1956.00 2253370.00 4815490.00 1956.00 1899.00 1975.00 1956.00 1975.00