Load time: 02/08/2011 10:28:59 AM Read time: 02/08/2011 10:28:59 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1357 Comment: standard, tsl Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 176186704 cps Min counts: 826 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34849616.00 32087158.00 19574280.00 176186704.00 74989744.00 17483230.00 117021.00 1957.00 1805.00 361956.00 479358.00 270744.00 B 46990068.00 32192904.00 9118738.00 104064824.00 25397968.00 24986714.00 115596.00 1938.00 2071.00 337514.00 308928.00 261146.00 C 19059684.00 13905584.00 6325029.00 75365064.00 11064599.00 8000183.00 120880.00 1919.00 2033.00 394742.00 521248.00 207529.00 D 21308002.00 11008802.00 3690733.00 16892740.00 14334034.00 15505932.00 114646.00 1900.00 2109.00 275686.00 880429.00 233264.00 E 4899382.00 28152000.00 3248393.00 15861667.00 7138056.00 7677712.00 137719.00 2109.00 2185.00 346713.00 851749.00 462119.00 F 3203087.00 2257707.00 1201142.00 6826222.00 5073906.00 7011113.00 116413.00 2014.00 2090.00 364636.00 544587.00 745237.00 G 7075600.00 1618285.00 3294152.00 3536045.00 3429534.00 3709063.00 121336.00 2052.00 1938.00 650548.00 483178.00 1748.00 H 1370070.00 912835.00 935718.00 1726250.00 1679299.00 1843107.00 138156.00 1938.00 2014.00 257630.00 633385.00 1653.00 Load time: 02/08/2011 10:31:35 AM Read time: 02/08/2011 10:31:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1358 Comment: standard, magic ampure Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 92313544 cps Min counts: 68679 counts Well list: r0(a1:h4,a7:h9) = cps(f2(a1:h4,a7:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62984808.00 92313544.00 66927320.00 140247.00 11844678.00 11917113.00 11683238.00 B 46966824.00 36898640.00 43027848.00 137358.00 10150128.00 10307922.00 9462566.00 C 29468378.00 22905370.00 23913920.00 137795.00 12713522.00 12808446.00 12361063.00 D 12553168.00 18515974.00 14446120.00 139962.00 11992454.00 12523464.00 11520887.00 E 12893317.00 15236504.00 12378950.00 152791.00 9865188.00 10235348.00 10217133.00 F 6438012.00 6041855.00 4569611.00 138423.00 10958765.00 11058500.00 10570561.00 G 3930984.00 3147638.00 2628912.00 139297.00 10906106.00 10824733.00 10398670.00 H 1997636.00 2953217.00 2729355.00 139430.00 10790554.00 11206014.00 11163126.00 Load time: 02/08/2011 10:33:31 AM Read time: 02/08/2011 10:33:31 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1359 Comment: t16 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18000758 cps Min counts: 778498 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18000758.00 12121672.00 14440466.00 17512808.00 17008592.00 14966462.00 B 15305973.00 10427418.00 9932653.00 17193712.00 15404618.00 13157514.00 C 16824290.00 9501324.00 13491381.00 4262069.00 17126986.00 13180995.00 D 12816605.00 14886416.00 12849478.00 16096787.00 17742990.00 13938823.00 E 10949409.00 13144519.00 10480707.00 17259006.00 17298354.00 12362810.00 F 13453587.00 11141080.00 13790793.00 15797646.00 14255762.00 13251535.00 G 17360034.00 17652708.00 9470174.00 12604549.00 14522600.00 14138247.00 H 13014344.00 16202956.00 11610613.00 15063477.00 14017302.00 11974028.00 Load time: 02/08/2011 10:34:43 AM Read time: 02/08/2011 10:34:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1360 Comment: t16 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18224476 cps Min counts: 778498 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17462642.00 11087575.00 14129113.00 16816404.00 17025964.00 14929896.00 17768422.00 12443512.00 15362273.00 17326574.00 17122764.00 14117505.00 B 14620296.00 10617184.00 10036310.00 17054794.00 15880661.00 13172965.00 15816165.00 10866222.00 10266556.00 17545844.00 15188615.00 12676694.00 C 16482054.00 9208913.00 13022464.00 4167131.00 17363248.00 13087793.00 16140606.00 10016670.00 13473449.00 4171512.00 17442142.00 12934147.00 D 12725097.00 14742029.00 12364892.00 15945812.00 17428318.00 15363517.00 13024578.00 15845170.00 13587974.00 16242812.00 17694596.00 14728502.00 E 11596275.00 13758815.00 10123216.00 17325032.00 17129962.00 12813736.00 11423963.00 14284718.00 10543246.00 17322062.00 16625772.00 12726179.00 F 13075615.00 10346610.00 13536468.00 15329844.00 14176454.00 13504698.00 13062231.00 11841939.00 14396404.00 15341082.00 14500906.00 13283031.00 G 17158428.00 17181266.00 9246188.00 12383223.00 14834081.00 14352214.00 17702604.00 18224476.00 9267741.00 12428713.00 14628839.00 14691644.00 H 12443633.00 15391248.00 11588715.00 14745464.00 14490810.00 11648677.00 13049729.00 16072559.00 11686430.00 14591195.00 14410555.00 11665416.00 Load time: 02/08/2011 10:36:21 AM Read time: 02/08/2011 10:36:21 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1361 Comment: t12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 5624819 cps Min counts: 778498 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4699475.00 4389721.00 4457414.00 4104821.00 2826853.00 4609730.00 3986269.00 4422877.00 2710684.00 4369841.00 4503771.00 4078349.00 B 4579637.00 5041596.00 4685691.00 2235248.00 3345507.00 4542701.00 4133983.00 4422707.00 4197337.00 4501108.00 4371551.00 3829759.00 C 4939765.00 4884145.00 4780980.00 3997817.00 4226989.00 4388174.00 4210119.00 4560998.00 4120616.00 4004165.00 4270025.00 4229287.00 D 4599464.00 4562835.00 4427516.00 3736535.00 4603166.00 4484614.00 4116099.00 4203817.00 4323935.00 4637357.00 2952379.00 4531787.00 E 4647353.00 4762426.00 4626278.00 4445778.00 4670934.00 4617733.00 3914972.00 4237833.00 4742347.00 4355343.00 4646828.00 4427042.00 F 4632241.00 5164265.00 4399641.00 4333900.00 4142937.00 4089137.00 3916721.00 4644187.00 4790615.00 4014501.00 4335194.00 3992488.00 G 4739477.00 5624819.00 4859926.00 4546935.00 3567044.00 4792435.00 4166926.00 4541126.00 4468779.00 4272487.00 4239885.00 3690576.00 H 4526733.00 5360257.00 4734761.00 4259027.00 4091316.00 5582152.00 3882127.00 4362433.00 4573269.00 4012643.00 4062288.00 3692905.00 Load time: 02/08/2011 10:38:10 AM Read time: 02/08/2011 10:38:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1362 Comment: t12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 6097808 cps Min counts: 778498 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 5351896.00 5444149.00 5166647.00 4546608.00 3317865.00 4934070.00 4665878.00 5157153.00 2891015.00 4428503.00 4785662.00 4312182.00 B 5448518.00 5445641.00 5318397.00 2536600.00 3574895.00 4801234.00 4743214.00 4910730.00 4246683.00 4900066.00 4636494.00 4519572.00 C 5925666.00 5527001.00 5428633.00 4558781.00 4930979.00 5198204.00 5553076.00 5070941.00 4605037.00 4701782.00 4456968.00 4623193.00 D 5293960.00 5763616.00 5253342.00 4343388.00 5495538.00 4896082.00 4663586.00 4778049.00 5077516.00 4992692.00 3294891.00 4712092.00 E 5153394.00 5554304.00 5468251.00 5019799.00 5264597.00 5279882.00 4325691.00 4578743.00 4896531.00 4588742.00 4951348.00 4473635.00 F 5371241.00 6097808.00 5358622.00 5107705.00 4656919.00 4664793.00 4718639.00 4447236.00 4751485.00 4589768.00 4674235.00 4386265.00 G 5742956.00 6096551.00 5733271.00 5150152.00 3954190.00 5237459.00 4732597.00 4740851.00 4794903.00 4349530.00 4482883.00 3716725.00 H 5783810.00 5897769.00 5132245.00 4738635.00 4630171.00 4632133.00 4602222.00 4421719.00 4769735.00 4362614.00 4680344.00 3899570.00 Load time: 02/08/2011 10:39:57 AM Read time: 02/08/2011 10:39:57 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1363 Comment: t12 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 6128655 cps Min counts: 778498 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4954957.00 5014661.00 4835049.00 4279863.00 3150457.00 4642815.00 4537434.00 4643727.00 2776548.00 4373793.00 4492092.00 3886694.00 B 4868726.00 5354277.00 5153483.00 2377238.00 3539513.00 4689363.00 4411992.00 4445215.00 4352244.00 4487670.00 4430943.00 4083937.00 C 5484021.00 5261604.00 5249120.00 4444169.00 4557860.00 4800486.00 4490217.00 4733904.00 4355166.00 4232357.00 3999440.00 4121331.00 D 4962167.00 5219102.00 5083763.00 3993232.00 5143786.00 4831646.00 4391784.00 4373602.00 4390641.00 4423860.00 2982491.00 4298629.00 E 4980121.00 5860678.00 5203335.00 4814132.00 4914046.00 4867219.00 3987113.00 4255595.00 4683177.00 4380995.00 4635718.00 4099859.00 F 5180362.00 5681310.00 4862880.00 4858459.00 4313068.00 4550068.00 4274376.00 4388389.00 4745504.00 4406026.00 4483746.00 3988258.00 G 5618526.00 6128655.00 5662075.00 5082880.00 3673663.00 4619503.00 4457736.00 4399649.00 4582821.00 4250669.00 4269266.00 3494963.00 H 5536214.00 5824861.00 5120848.00 4892109.00 4286947.00 4590593.00 4405206.00 4445791.00 4667375.00 4791149.00 4015360.00 3663496.00