Load time: 04/19/2011 11:05:46 AM Read time: 04/19/2011 11:05:46 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1450 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 183979360 cps Min counts: 68394 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 183979360.00 160944928.00 159470608.00 141434.00 B 109385400.00 90213768.00 92166888.00 141396.00 C 68724560.00 52386700.00 51764996.00 298329.00 D 39229940.00 28765480.00 31656050.00 340371.00 E 26448192.00 20087026.00 20500292.00 136788.00 F 14856304.00 10873542.00 11812531.00 139016.00 G 7330070.00 6730501.00 7713374.00 138749.00 H 3794172.00 4279794.00 4463776.00 142253.00 Load time: 04/19/2011 11:07:30 AM Read time: 04/19/2011 11:07:30 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1451 Comment: 393 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19871202 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14935595.00 13788776.00 11353663.00 13319130.00 14161999.00 15767546.00 17282988.00 14183814.00 12706683.00 13545177.00 15239791.00 14650981.00 B 14462781.00 13135341.00 11205447.00 14588033.00 14069439.00 15018745.00 14702409.00 14120281.00 12433690.00 14297653.00 13922932.00 15450157.00 C 15304807.00 16318025.00 14743627.00 14859248.00 14523460.00 16873396.00 15544355.00 15631246.00 15319416.00 14463988.00 13441295.00 14583096.00 D 15119662.00 13997346.00 14735049.00 15478521.00 14865196.00 15068220.00 15644290.00 16270943.00 14681762.00 16394968.00 13958665.00 15959200.00 E 15619225.00 15327274.00 15240773.00 14731402.00 14628008.00 15823592.00 16120475.00 16280520.00 15665627.00 18758766.00 15683363.00 14976466.00 F 13341527.00 13114512.00 13581054.00 13313332.00 14379160.00 15618661.00 19871202.00 17028352.00 12136345.00 16805260.00 15357790.00 17247634.00 G 12996825.00 12739040.00 11953073.00 13828136.00 16509496.00 14998441.00 16370567.00 15804098.00 15087692.00 15020887.00 13537019.00 13927158.00 H 14100830.00 12983129.00 12837214.00 14871630.00 14215665.00 15748157.00 15227532.00 15563650.00 16743855.00 15319989.00 13419624.00 12894647.00 Load time: 04/19/2011 11:09:34 AM Read time: 04/19/2011 11:09:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1452 Comment: 394 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26590576 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21335280.00 20718898.00 22671056.00 21366788.00 24053832.00 22658802.00 25469342.00 22015038.00 21277542.00 20986172.00 19801478.00 17265426.00 B 20174790.00 22662292.00 19345762.00 21683234.00 22260986.00 25032078.00 23403622.00 21886166.00 20756338.00 18244076.00 19399898.00 19340628.00 C 21370242.00 22586358.00 22399470.00 23199434.00 24295108.00 25140934.00 18580052.00 21652954.00 21604370.00 19579664.00 18770280.00 21497594.00 D 22222230.00 22881304.00 22796564.00 23200328.00 25605392.00 24470894.00 22541380.00 23553104.00 22482768.00 22059374.00 19640808.00 24079898.00 E 22106332.00 22756520.00 24784994.00 21410544.00 23416830.00 24449414.00 23462624.00 23154800.00 24206280.00 23531360.00 19038884.00 21907746.00 F 21138782.00 20829570.00 21653558.00 22399276.00 23670556.00 26289588.00 26590576.00 23474414.00 22860378.00 22874324.00 25442090.00 22648014.00 G 21818630.00 21091678.00 21738598.00 22131800.00 19458076.00 25201374.00 24544286.00 23808936.00 22716288.00 21855438.00 18609178.00 20185860.00 H 20680604.00 22798898.00 21589600.00 23258240.00 24328454.00 24479498.00 25034568.00 24466670.00 20408560.00 22009446.00 21350234.00 22020694.00 Load time: 04/19/2011 11:11:34 AM Read time: 04/19/2011 11:11:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1453 Comment: 395 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26829876 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20047374.00 18363238.00 21782190.00 21721220.00 23779176.00 23278166.00 23786788.00 22404038.00 18588244.00 18403062.00 19306108.00 18637150.00 B 20015732.00 18778188.00 17338124.00 22130418.00 23874604.00 23343378.00 22436778.00 23573184.00 20231312.00 19306922.00 17997202.00 18062350.00 C 25899258.00 22228184.00 21221032.00 20983742.00 24889560.00 26446398.00 25903042.00 22925698.00 23826248.00 18869462.00 18091468.00 19298704.00 D 22954168.00 22155628.00 22765358.00 25482158.00 22845312.00 26829876.00 22160160.00 25326588.00 22888488.00 20347222.00 17971282.00 20575414.00 E 20922498.00 23148478.00 23249784.00 23491176.00 18190970.00 24625892.00 22963768.00 24150288.00 21228370.00 19786056.00 17977164.00 19405110.00 F 21924126.00 20547444.00 20025704.00 21945038.00 22059124.00 24363720.00 22732708.00 21704056.00 20702124.00 18324320.00 19603434.00 19166100.00 G 23116092.00 19927510.00 19138490.00 21419426.00 20532242.00 22231100.00 21102694.00 20707180.00 19710100.00 19265332.00 16779624.00 20371242.00 H 20498674.00 19982706.00 19875304.00 21605928.00 22279430.00 23227900.00 22175410.00 21585772.00 20247436.00 19082020.00 18763820.00 16905912.00 Load time: 04/19/2011 11:13:17 AM Read time: 04/19/2011 11:13:17 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1454 Comment: 396 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22716090 cps Min counts: 87463 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14388496.00 12312868.00 12312090.00 13274809.00 14055011.00 14662137.00 14864488.00 16291674.00 12492423.00 12306982.00 15294873.00 3241466.00 B 17472952.00 17745042.00 16443880.00 19731692.00 21260200.00 22716090.00 21782432.00 18146024.00 18254966.00 18360558.00 18661722.00 18536906.00 C 13677802.00 14693905.00 15067289.00 14469005.00 19486956.00 18550884.00 584949.00 15909163.00 14701993.00 13863682.00 15044182.00 174926.00 D 9861056.00 12502355.00 12633897.00 13813270.00 14983169.00 15918189.00 17249922.00 15873932.00 12724885.00 14399974.00 12813739.00 12960713.00 E 9787277.00 10929618.00 11059451.00 12675415.00 13811045.00 13653498.00 14149999.00 14341797.00 14384729.00 13736585.00 11629276.00 13344197.00 F 8876308.00 9569152.00 8054410.00 10810211.00 12715715.00 14014878.00 14309509.00 14057113.00 13735568.00 13536502.00 13020563.00 12785549.00 G 9554019.00 8480365.00 8121324.00 10158160.00 11509469.00 12927242.00 16364625.00 13657898.00 11602978.00 11703988.00 10410585.00 11025571.00 H 10889407.00 11649584.00 10910598.00 11349268.00 13467523.00 12819236.00 13828775.00 14626253.00 12287785.00 11444211.00 13499974.00 11331640.00 Load time: 04/19/2011 11:15:00 AM Read time: 04/19/2011 11:15:00 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1455 Comment: 397 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33217918 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28387402.00 27276816.00 27232910.00 30386064.00 30257098.00 31929638.00 31875610.00 32407542.00 30161962.00 28582362.00 28317724.00 23871242.00 B 26461472.00 20024880.00 20384768.00 22695200.00 25737380.00 23354632.00 24598088.00 26067108.00 25353098.00 19848798.00 30416874.00 30790582.00 C 29186824.00 29782330.00 29492330.00 30315788.00 33217918.00 32654770.00 29451010.00 32467306.00 29722970.00 27785050.00 28023362.00 26986400.00 D 24872100.00 22778186.00 26133838.00 24918668.00 31524768.00 31368680.00 25448816.00 31581188.00 23105614.00 23044104.00 20016346.00 24902118.00 E 24355806.00 24213868.00 25128460.00 24610276.00 25301364.00 25789806.00 24920100.00 25347330.00 24123470.00 23297634.00 19980348.00 22110090.00 F 23672494.00 21885122.00 19916570.00 21051426.00 23673646.00 25692528.00 25312122.00 23184122.00 20665864.00 20813894.00 20993400.00 10540566.00 G 27893566.00 19126102.00 16704477.00 23012418.00 20994870.00 22631384.00 23371078.00 20972344.00 19107638.00 18698590.00 18334102.00 21813018.00 H 22927314.00 20848390.00 19983522.00 23466928.00 22255970.00 23543296.00 23961526.00 21910576.00 18125652.00 19946980.00 20062948.00 18748032.00 Load time: 04/19/2011 11:16:38 AM Read time: 04/19/2011 11:16:38 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1456 Comment: 398 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 47284856 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35200392.00 28431486.00 34554112.00 33563008.00 31206890.00 37105032.00 36587692.00 34068228.00 30645998.00 29247130.00 26414334.00 25807046.00 B 30354016.00 28521218.00 27625514.00 39736028.00 31468164.00 36073736.00 36428080.00 32704478.00 30212440.00 30825388.00 26550656.00 23662438.00 C 33301034.00 33807572.00 29939306.00 37925284.00 33235332.00 40155852.00 42650492.00 35294700.00 34305900.00 32755024.00 28294302.00 34112488.00 D 27707464.00 26814288.00 29400050.00 39640896.00 32745948.00 36863528.00 36037900.00 33770972.00 33305584.00 35126356.00 28553272.00 32663522.00 E 26025612.00 28032428.00 28771528.00 36858476.00 30991978.00 34998520.00 36537468.00 32939848.00 33948112.00 33485478.00 26632616.00 29202560.00 F 27719382.00 22757386.00 21485336.00 33048026.00 30727622.00 37664108.00 36680100.00 33726584.00 30460714.00 31279608.00 31351534.00 28586974.00 G 24766420.00 21174942.00 18110626.00 35820732.00 28647348.00 31352538.00 35460952.00 33668780.00 47284856.00 28152742.00 25156828.00 25388092.00 H 23873144.00 24375978.00 22116738.00 43521836.00 30211504.00 32159122.00 35110856.00 33296202.00 28655246.00 31194156.00 24472504.00 16563071.00 Load time: 04/19/2011 11:18:14 AM Read time: 04/19/2011 11:18:14 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1457 Comment: 399 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40672472 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 31890992.00 30870898.00 29840332.00 33021860.00 34123380.00 35422300.00 32242348.00 31850746.00 30873708.00 28999508.00 26459408.00 22404616.00 B 30725924.00 30500620.00 30343386.00 32927752.00 34257232.00 38096648.00 33087988.00 29313418.00 27818850.00 26653826.00 25922156.00 29574306.00 C 34630212.00 35087640.00 34244892.00 35191496.00 31907040.00 37459868.00 35854496.00 35389344.00 31555762.00 27630798.00 25943886.00 27381876.00 D 34425228.00 35025712.00 34634824.00 37322192.00 39685072.00 40672472.00 37819172.00 36595076.00 33349024.00 30898542.00 27615340.00 31776258.00 E 33770392.00 36841936.00 37068000.00 34149528.00 37321476.00 36474756.00 36658720.00 35155492.00 32977876.00 37615608.00 29022924.00 29492442.00 F 33601180.00 32122960.00 33997984.00 35192768.00 36189568.00 39848504.00 38374452.00 35423424.00 31963662.00 30637450.00 29409476.00 32998664.00 G 32079752.00 31687436.00 32527022.00 33417042.00 35081168.00 38094784.00 38645224.00 34530088.00 33191622.00 28122692.00 29502370.00 26721130.00 H 33388138.00 33689284.00 30070092.00 32887066.00 33534576.00 35921872.00 34686116.00 34589664.00 34368492.00 32098504.00 31450144.00 29323890.00 Load time: 04/19/2011 11:19:59 AM Read time: 04/19/2011 11:19:59 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1458 Comment: 402 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30253224 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24307072.00 22230464.00 23493790.00 24148418.00 25019634.00 26502656.00 25811486.00 28137102.00 28018026.00 24743474.00 25775998.00 18906422.00 B 25260714.00 26448730.00 20360500.00 24106098.00 25228766.00 28765162.00 26660568.00 25407116.00 24081312.00 23439846.00 21876282.00 20813502.00 C 26169180.00 23028522.00 21999950.00 24149092.00 25551788.00 26631346.00 27520818.00 25498322.00 28250742.00 22123766.00 21650672.00 20896654.00 D 26590486.00 24483798.00 24373996.00 21376450.00 28985714.00 27012850.00 24540888.00 28519860.00 27547744.00 25743158.00 21390756.00 22290060.00 E 24753136.00 29535888.00 24341514.00 25348484.00 26402352.00 29248010.00 29247680.00 27105608.00 26394132.00 27708350.00 21033600.00 22167280.00 F 24542742.00 24261022.00 22824652.00 24006446.00 27400922.00 30253224.00 26722778.00 26717926.00 22791366.00 24697170.00 25416388.00 23208616.00 G 21056768.00 20956002.00 20334382.00 23392670.00 23824066.00 24464984.00 26796414.00 26122636.00 23281568.00 21326940.00 19532882.00 20369542.00 H 21903932.00 22853344.00 20258586.00 23197502.00 24717206.00 23767144.00 25004230.00 26079484.00 22324580.00 21136090.00 22846918.00 19627312.00 Load time: 04/19/2011 11:21:37 AM Read time: 04/19/2011 11:21:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1459 Comment: 403 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 11198030 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8205030.00 6941984.00 8410296.00 7662561.00 8188085.00 6990449.00 7119531.00 7585419.00 7445049.00 7200213.00 7419325.00 6527133.00 B 7396661.00 7862813.00 7315946.00 7148066.00 9007882.00 7478524.00 8239417.00 7465168.00 7913138.00 9044821.00 8049315.00 6898626.00 C 10237861.00 7769499.00 9642754.00 7416496.00 8688706.00 7806415.00 8351693.00 6713433.00 9122284.00 7732822.00 8268129.00 9145153.00 D 7698405.00 7834230.00 8483381.00 8565135.00 7376687.00 7657678.00 6836653.00 7889507.00 8870220.00 7734601.00 6361129.00 7707565.00 E 8146067.00 8567967.00 8959557.00 8716839.00 7383000.00 7189692.00 6994361.00 7363393.00 9023515.00 8437265.00 6995108.00 7042220.00 F 8873872.00 8358763.00 8799025.00 8514352.00 8706417.00 8769601.00 7365694.00 8151738.00 9032303.00 8941979.00 8435266.00 8171065.00 G 8571254.00 7705608.00 7480749.00 8787099.00 9111095.00 8245723.00 9164777.00 8797964.00 7783192.00 8708712.00 7800153.00 6138621.00 H 7853478.00 8356534.00 7688099.00 8422103.00 8473041.00 8649622.00 8756722.00 8877934.00 7972666.00 11198030.00 7959168.00 6400954.00 Load time: 04/19/2011 11:23:17 AM Read time: 04/19/2011 11:23:17 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1460 Comment: 404 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24144828 cps Min counts: 779907 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23305292.00 20154510.00 20144774.00 22049332.00 23913550.00 19925066.00 22932976.00 19469056.00 22216278.00 20911950.00 20630712.00 15928757.00 B 20434064.00 16347987.00 16251243.00 15179399.00 21262284.00 15843136.00 20604496.00 15934451.00 18407014.00 18068626.00 18780132.00 19575584.00 C 16212057.00 16225211.00 14989563.00 14993915.00 15679011.00 16502266.00 15292022.00 15019035.00 17591026.00 15251086.00 15050742.00 14832373.00 D 16500170.00 19854156.00 15736080.00 15704205.00 20281504.00 18958448.00 15683866.00 17378844.00 21385358.00 15529992.00 15274712.00 13872459.00 E 19110448.00 15018573.00 18685958.00 17950684.00 16845078.00 20853684.00 14905708.00 16727264.00 18763684.00 22266134.00 17198948.00 15086233.00 F 16627836.00 15334327.00 18328240.00 14906649.00 16045815.00 18304968.00 16572643.00 16019482.00 17039176.00 16417505.00 18302304.00 16266089.00 G 15092684.00 13233229.00 14472873.00 17241608.00 16060025.00 16885086.00 16688998.00 20970202.00 17779546.00 16168867.00 14637206.00 13333931.00 H 16569897.00 22312254.00 20787210.00 24144828.00 16552138.00 18188214.00 14358828.00 17464108.00 18781174.00 18202138.00 17565512.00 13776159.00