Load time: 04/20/2011 11:35:51 AM Read time: 04/20/2011 11:35:51 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1462 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 202884432 cps Min counts: 67779 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 202884432.00 175434896.00 173977936.00 283732.00 B 117247824.00 94861824.00 98656736.00 136738.00 C 68294296.00 53225740.00 54433648.00 135559.00 D 39599952.00 30959594.00 32333392.00 143169.00 E 24708444.00 21287446.00 21693122.00 139706.00 F 16033317.00 12312919.00 12610961.00 138755.00 G 7794295.00 7955354.00 6931510.00 140277.00 H 4941807.00 4762698.00 4669746.00 137404.00 Load time: 04/20/2011 11:37:31 AM Read time: 04/20/2011 11:37:31 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1463 Comment: 405 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28492536 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19796054.00 21029010.00 18934886.00 21943688.00 24438948.00 25531046.00 22415240.00 24536746.00 20223472.00 17843104.00 19029442.00 17697674.00 B 20158448.00 20378796.00 18486654.00 22600440.00 23683312.00 26849616.00 27131356.00 24737780.00 22099884.00 18756470.00 19109232.00 19719514.00 C 24385490.00 22772680.00 20235970.00 24121984.00 24402748.00 28171588.00 28077904.00 24330902.00 25079874.00 20033714.00 19707792.00 19318374.00 D 22517702.00 22307700.00 23227586.00 23885912.00 25980544.00 26253798.00 24764112.00 25273128.00 23711416.00 21867152.00 19642522.00 18233618.00 E 22202894.00 23925072.00 23009630.00 23848632.00 23360994.00 24527558.00 24314430.00 24079056.00 26290732.00 23702908.00 20789578.00 18237330.00 F 20698524.00 20518732.00 20885074.00 19170392.00 24844324.00 28492536.00 25089484.00 23435250.00 21063228.00 18636972.00 19016716.00 18203506.00 G 13742463.00 16745046.00 18085178.00 20059342.00 21683164.00 22937504.00 22076096.00 20795292.00 19186296.00 17577282.00 15599903.00 19598556.00 H 18434048.00 19957318.00 16609178.00 20799900.00 20555534.00 24021756.00 23193366.00 21386116.00 21440954.00 18815474.00 14861347.00 17042658.00 Load time: 04/20/2011 11:39:14 AM Read time: 04/20/2011 11:39:14 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1464 Comment: 406 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28960226 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24116090.00 22839688.00 21189164.00 23205106.00 22607850.00 26159062.00 24386102.00 20755912.00 24301086.00 22394818.00 20447182.00 17483742.00 B 25715632.00 21743298.00 22153662.00 23649318.00 24884466.00 26745124.00 24902916.00 23711922.00 21559052.00 20601180.00 19273748.00 20174990.00 C 24633700.00 22120900.00 23397116.00 22515748.00 25588048.00 25291418.00 24646322.00 22867908.00 22872404.00 21010698.00 21020218.00 23322332.00 D 24359730.00 23125230.00 23701320.00 25192976.00 26215294.00 28960226.00 23633610.00 26166000.00 23996678.00 27123424.00 21194754.00 22725464.00 E 24622804.00 23972320.00 27251754.00 24577296.00 25267964.00 27351420.00 24385034.00 24212370.00 24533812.00 25440364.00 21121458.00 21016192.00 F 25306940.00 20727152.00 23904450.00 22530592.00 24418806.00 26979660.00 25383682.00 23327290.00 24284502.00 21601782.00 23117546.00 23052646.00 G 22910664.00 20296792.00 23333504.00 24162598.00 23984714.00 25013944.00 24712518.00 23921544.00 25225024.00 21514534.00 23334132.00 21550230.00 H 21918694.00 21811214.00 22169294.00 25568824.00 23806814.00 25116730.00 25541920.00 24685276.00 24211920.00 21704118.00 23350772.00 21428574.00 Load time: 04/20/2011 11:40:52 AM Read time: 04/20/2011 11:40:52 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1465 Comment: 407 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31497408 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 23665404.00 21897260.00 22981268.00 26307242.00 26356352.00 28107980.00 27608000.00 27604970.00 24843294.00 22474710.00 24291390.00 15746125.00 B 21908034.00 19467058.00 20310176.00 23209944.00 27547204.00 28649450.00 30822816.00 26357156.00 22963390.00 21878572.00 22512176.00 21177420.00 C 25555824.00 24450372.00 20387110.00 21258008.00 27290982.00 31497408.00 31246592.00 25326350.00 25531462.00 23613702.00 22671978.00 23044946.00 D 20570728.00 17863186.00 19099116.00 22615196.00 25600320.00 25923244.00 28176580.00 25075756.00 24401376.00 26924570.00 22101764.00 22443836.00 E 20545402.00 19820372.00 21286574.00 23596898.00 24857718.00 25999266.00 27206660.00 26060300.00 26285148.00 28093288.00 22422398.00 22161664.00 F 20479368.00 17112526.00 15654743.00 18404748.00 26143090.00 29939890.00 28373034.00 25036606.00 23939254.00 21668874.00 22796996.00 24094690.00 G 15452662.00 12866032.00 13037758.00 17227416.00 24966102.00 25346780.00 24952192.00 23054216.00 22993878.00 20880598.00 20123674.00 18619382.00 H 18497008.00 17289754.00 14350074.00 19679526.00 24872870.00 27985440.00 27102578.00 24706408.00 24419038.00 25424844.00 23004536.00 21433582.00 Load time: 04/20/2011 11:42:26 AM Read time: 04/20/2011 11:42:26 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1466 Comment: 408 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 39090840 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 31081962.00 30429852.00 32236288.00 34599240.00 32414084.00 36171612.00 32403034.00 34689956.00 32347886.00 31151416.00 30339342.00 16764462.00 B 29841520.00 30347020.00 29775388.00 32418400.00 33255072.00 37871980.00 27987854.00 31975320.00 31473618.00 28482748.00 17834284.00 29641864.00 C 32479468.00 30651632.00 29717258.00 28679182.00 34689488.00 34940832.00 33677156.00 31989762.00 36187744.00 28681922.00 27287730.00 30120064.00 D 31816322.00 32884190.00 32424602.00 34889992.00 37137612.00 35059672.00 32713982.00 39090840.00 30597836.00 33167744.00 29817202.00 32536558.00 E 28750706.00 31829574.00 32142288.00 32561302.00 33252374.00 36145612.00 33827428.00 30097958.00 31642822.00 31756550.00 27177342.00 28723364.00 F 29509526.00 27832020.00 31243084.00 30282280.00 36477740.00 35897032.00 33186244.00 32232954.00 31556972.00 30083784.00 31581652.00 28746464.00 G 29256052.00 30348912.00 32810118.00 32057402.00 33895108.00 35089032.00 34518472.00 33116720.00 32036052.00 29816402.00 28246742.00 28002434.00 H 27722418.00 30370912.00 32709590.00 32523388.00 32902930.00 35292600.00 35016356.00 34619068.00 34910312.00 32517148.00 29906568.00 27099184.00 Load time: 04/20/2011 11:44:02 AM Read time: 04/20/2011 11:44:02 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1467 Comment: 409 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26535936 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19852636.00 20343258.00 20538092.00 23403016.00 25103060.00 24290558.00 26494436.00 24034276.00 24231040.00 22346400.00 21307704.00 17858724.00 B 18592152.00 19126306.00 17342238.00 21629002.00 23650682.00 24304572.00 23000598.00 26535936.00 21809870.00 21323970.00 19178176.00 20240280.00 C 20450510.00 21085566.00 18049198.00 20810062.00 21930966.00 25822058.00 24000448.00 23789154.00 22374692.00 20367716.00 18040342.00 19354600.00 D 19460834.00 17803890.00 17448356.00 22716986.00 21351194.00 25387320.00 22429368.00 24175266.00 23604616.00 26095816.00 20490460.00 20108616.00 E 17012820.00 18308628.00 20072620.00 20522136.00 21154366.00 25106134.00 22814482.00 22749682.00 22631880.00 24344814.00 18709186.00 17848622.00 F 18169638.00 16392049.00 15984251.00 19306074.00 21815792.00 25905924.00 21958592.00 24201392.00 22517506.00 21869300.00 20752320.00 20473450.00 G 15395575.00 14021143.00 13939558.00 17544556.00 19797562.00 22416658.00 22712882.00 22255014.00 22816356.00 20159176.00 18578190.00 19383580.00 H 16392118.00 16724131.00 12782588.00 17896702.00 22791396.00 23784218.00 23419546.00 23545994.00 22330646.00 21417384.00 19472116.00 19841416.00 Load time: 04/20/2011 11:45:37 AM Read time: 04/20/2011 11:45:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1468 Comment: 410 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38587580 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28854606.00 32944662.00 31715708.00 32785826.00 34054336.00 32155416.00 34624908.00 35046904.00 31542024.00 29295096.00 28670846.00 23121662.00 B 31407278.00 29296968.00 27551882.00 31936534.00 33655476.00 35703460.00 34606608.00 32750552.00 34368976.00 28376026.00 28119136.00 29847922.00 C 30351986.00 32682972.00 30284164.00 30594234.00 36589744.00 35879676.00 38587580.00 31950808.00 35541600.00 27354108.00 29535474.00 30668522.00 D 29315042.00 30424624.00 30899564.00 33760456.00 38186908.00 29118320.00 33079890.00 33960692.00 34123372.00 33052672.00 28063848.00 29327068.00 E 28642604.00 32524742.00 34162864.00 29906224.00 33290160.00 33825812.00 35426240.00 32633434.00 31604066.00 32581584.00 26339872.00 28528002.00 F 31578580.00 30746930.00 28270204.00 27436158.00 31563614.00 34433064.00 33646036.00 32580766.00 23821806.00 29429956.00 28775016.00 30560160.00 G 27812054.00 26817648.00 26124250.00 30609252.00 30677816.00 32810808.00 32015122.00 30245494.00 29463558.00 28945596.00 28016730.00 27571766.00 H 27718276.00 27876724.00 30346548.00 30851122.00 30984580.00 31419816.00 32069274.00 33468014.00 31372004.00 29073236.00 30388438.00 27826754.00 Load time: 04/20/2011 11:47:13 AM Read time: 04/20/2011 11:47:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1469 Comment: 411 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 44785000 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36531424.00 30878628.00 32519450.00 35342212.00 37247128.00 37136552.00 39881036.00 36881008.00 37868120.00 33177066.00 31002206.00 26347264.00 B 30234348.00 31016028.00 29535364.00 34294108.00 36471932.00 40842496.00 40150204.00 36803844.00 37152488.00 32719750.00 27940690.00 32105210.00 C 33246702.00 34382928.00 31092132.00 35175352.00 38178120.00 42208480.00 44785000.00 34809576.00 35436228.00 30034858.00 26732002.00 31725264.00 D 34471600.00 36672740.00 34428952.00 40324720.00 37673340.00 42946068.00 39640644.00 38758776.00 37820156.00 35436552.00 31338446.00 34716684.00 E 33740432.00 35595016.00 36128352.00 39762400.00 40551972.00 37793744.00 38678360.00 35086036.00 33669440.00 37957572.00 33961872.00 32727448.00 F 33819796.00 31738958.00 31739478.00 35347988.00 37363208.00 39851264.00 38786744.00 35858704.00 32073760.00 35256032.00 33245568.00 33823904.00 G 31453166.00 29175442.00 34304376.00 33640808.00 35604772.00 40576252.00 36456408.00 36713856.00 37089536.00 30828912.00 30254418.00 30058024.00 H 30496180.00 32761290.00 35782804.00 35403068.00 42632424.00 39752264.00 42279928.00 44364332.00 34554288.00 40147516.00 32956504.00 37445432.00 Load time: 04/20/2011 11:48:47 AM Read time: 04/20/2011 11:48:47 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1470 Comment: 412 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42462616 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27706648.00 27266916.00 26619142.00 33574860.00 35251248.00 35685640.00 34282344.00 35613716.00 34205304.00 31328492.00 28289812.00 21853050.00 B 27407018.00 27605170.00 28547230.00 30360500.00 33481674.00 33963204.00 33576304.00 33440868.00 26665230.00 30807584.00 28206870.00 25636198.00 C 26971538.00 30969928.00 30529750.00 31321314.00 31918092.00 34934096.00 37014488.00 34169160.00 32449716.00 28546184.00 28347332.00 25892150.00 D 33909116.00 33123338.00 32417452.00 34765628.00 35080816.00 38140376.00 33469882.00 35338692.00 33924176.00 32095824.00 28853430.00 25978982.00 E 29063260.00 35754896.00 32370054.00 34102168.00 32805974.00 34849736.00 34110976.00 31656832.00 42462616.00 35849632.00 30197092.00 26394204.00 F 29457992.00 28844244.00 29195334.00 30223326.00 34309360.00 34630296.00 33685444.00 33250956.00 29537376.00 29336562.00 29225224.00 26998448.00 G 29704230.00 27542140.00 26518418.00 29318240.00 31518044.00 34518316.00 33224304.00 32046196.00 28047384.00 27081292.00 26106396.00 24681604.00 H 26619688.00 28384710.00 24922346.00 30631272.00 31064862.00 32111958.00 37194512.00 34318124.00 28532178.00 30002824.00 29708420.00 27717490.00 Load time: 04/20/2011 11:50:21 AM Read time: 04/20/2011 11:50:21 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1471 Comment: 413 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32898486 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19730746.00 20572682.00 20526676.00 25167510.00 23205520.00 23172744.00 23989884.00 23072304.00 21029350.00 14410788.00 18015026.00 13257788.00 B 19891656.00 21264910.00 20425478.00 24931676.00 23586402.00 23927864.00 23726070.00 23330150.00 18673546.00 18373376.00 16605499.00 17238506.00 C 22032656.00 23356652.00 21938128.00 23980582.00 24899016.00 24470638.00 25121024.00 22979302.00 24636344.00 19354552.00 18954506.00 20208054.00 D 23291730.00 24600960.00 23004874.00 27431524.00 26047146.00 26365626.00 25856500.00 24801154.00 24493714.00 22513150.00 18744962.00 23086454.00 E 20492940.00 27095320.00 24509430.00 25715716.00 25823342.00 27654636.00 26221910.00 25820604.00 23497360.00 23311728.00 19920080.00 20092754.00 F 21667188.00 23467218.00 21475992.00 22448748.00 25721996.00 26068754.00 24805416.00 26210532.00 24083594.00 21331498.00 21844778.00 20876682.00 G 19535328.00 19962124.00 20662686.00 20579420.00 23388688.00 24270434.00 27392760.00 24396182.00 24021386.00 23809506.00 20385884.00 17929106.00 H 19722008.00 23067182.00 19607434.00 25180440.00 23456412.00 32898486.00 24804628.00 26907928.00 23663032.00 21576302.00 22059474.00 20246276.00 Load time: 04/20/2011 11:51:54 AM Read time: 04/20/2011 11:51:54 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1472 Comment: 431 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40697036 cps Min counts: 779299 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28739678.00 25869030.00 25736694.00 30132876.00 31596376.00 34500744.00 31764058.00 33571968.00 28896544.00 31301186.00 31876314.00 25632486.00 B 31738958.00 29117012.00 24506654.00 27756978.00 28787242.00 37363388.00 37089184.00 34150440.00 31170852.00 31112738.00 27016886.00 30012994.00 C 27935882.00 27575372.00 24450218.00 26481922.00 34452240.00 37191848.00 35046900.00 29610214.00 33454798.00 29922644.00 25607388.00 29155794.00 D 23485748.00 22827780.00 25732360.00 27488710.00 31102804.00 40697036.00 31150668.00 33484986.00 31413734.00 34302716.00 27659938.00 31763282.00 E 23544998.00 31451136.00 25956746.00 31624328.00 29290694.00 36428452.00 34581276.00 27802730.00 31048776.00 33173250.00 24145904.00 32639856.00 F 28450306.00 19361234.00 19278564.00 21094642.00 29135846.00 33380278.00 34677912.00 34559344.00 34338540.00 26834362.00 29758788.00 31980310.00 G 18359092.00 17427010.00 16583802.00 23306706.00 25179058.00 28279852.00 35831012.00 31096474.00 28532178.00 27142694.00 26763400.00 24193204.00 H 25924970.00 24724434.00 20524894.00 29619670.00 31393490.00 30622484.00 32027364.00 36136392.00 32197132.00 28997078.00 32283962.00 28224542.00