Load time: 5/2/2011 9:46 Read time: 5/2/2011 9:46 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1517 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 79550008 cps Min counts: 63017 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 75114320 79550008 75672696 127385 B 42290736 43976284 44887788 127708 C 26027124 27992206 27946598 127385 D 16388278 17124924 17637022 131359 E 10290940 11201690 11432840 137120 F 6449007 6536228 6829412 128374 G 4068029 4196275 4431049 129305 H 2592169 2608608 2664584 126035 Load time: 5/2/2011 9:48 Read time: 5/2/2011 9:48 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1518 Comment: 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27442868 cps Min counts: 778762 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19760786 19772830 18170428 27442868 27133458 22037046 20699666 22140482 17836414 22210512 25302578 21699748 B 17615042 16692733 19955688 16384175 18403026 23124814 20946026 19154976 18109752 16619882 14364500 17399680 C 19069422 18517896 17668876 18811330 19818118 22378368 17482802 17860424 19033998 19087182 17293926 18744364 D 18585068 15856969 16781944 17832452 19233128 20456344 18616836 16766155 15858293 14847151 17955468 18319950 E 18161444 15987510 14039275 20876510 20611350 16351697 19361804 17053448 18001706 18510282 19302200 14805432 F 18333666 15220704 16664727 19275350 20766288 20007420 18527678 18325338 19224438 16665263 16858818 13778706 G 18404202 16790084 15142806 14120249 19533724 19326440 18051110 19293356 17211596 14434792 16046807 18378880 H 17418750 12843072 15729562 21277962 20785410 13207741 17583502 17460070 12005937 19385952 15337946 15118907 Load time: 5/2/2011 9:49 Read time: 5/2/2011 9:49 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1519 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21842594 cps Min counts: 778762 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15885692 16080604 13992759 19741752 21374794 16860424 17288550 15430975 12801187 16886932 18910500 15911106 B 16642969 16297767 16669330 15137596 18033302 20275106 20866498 18242020 16567657 15260641 13671908 15755499 C 18826474 18684866 17873010 17144264 20635764 21842594 17579850 17919564 18292668 18230404 17355282 16862176 D 18216546 15942408 16531396 15956618 17180270 18988518 19266864 15977602 15746864 14999543 17482178 18035850 E 18417564 16451912 14154510 19125244 20711774 15804002 17433570 16123687 16898582 18232110 19949350 14417555 F 18361458 15123045 17196810 18020450 21458958 19706132 18085484 15921745 17182772 17073150 16234676 12906073 G 17319772 16038082 13681275 13168790 19821292 20194978 16155126 18232710 14955758 13479607 15736395 18233008 H 17510046 13373817 16076852 19338628 19935254 14443116 16859328 17545076 10822944 19575560 16176974 15223352 Load time: 5/2/2011 9:51 Read time: 5/2/2011 9:51 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1520 Comment: 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21582240 cps Min counts: 778762 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14694495 14513694 14052752 19774184 20831722 16178286 16464295 15581394 12456159 17329600 18387342 15289254 B 16463947 15387040 16640763 15198515 17477508 19395368 19726300 16187161 15829476 15446432 13550558 15564517 C 16553042 17054270 16766227 17745736 19388076 21582240 17015888 17674570 17496394 17595658 17107318 16242735 D 17467548 15380750 16175091 16480077 18400550 20572038 17013880 16078877 16086053 14773161 16494354 17195786 E 16939862 16036563 13540121 19392856 20316152 15972982 17457760 15905420 16475022 16539017 19201260 14112060 F 18115902 14344312 17092412 18059692 20809458 19727698 17914330 16913448 17312764 17299494 16280666 13133035 G 17192332 15258309 13140902 12595388 19439182 19512924 15888578 18330818 14715404 13817870 15251913 18385868 H 16102082 12424006 14491898 18165596 18273310 13710177 15795348 16228586 10501145 19015084 15025908 14315675