Load time: 09/30/2011 01:47:10 PM Read time: 09/30/2011 01:47:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1898 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 87399072 cps Min counts: 65027 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 60951936.00 62432996.00 49144940.00 132911.00 B 76048048.00 39199924.00 31361330.00 134130.00 C 27411600.00 22029140.00 28530154.00 148775.00 D 15246323.00 21199262.00 11971921.00 140776.00 E 7197858.00 8633158.00 11242270.00 142186.00 F 6062291.00 7085805.00 6184452.00 130054.00 G 5824024.00 6294367.00 4527184.00 133044.00 H 2002354.00 1508820.00 2101421.00 87399072.00 Load time: 09/30/2011 01:48:07 PM Read time: 09/30/2011 01:48:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1899 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 101930352 cps Min counts: 67865 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 63135892.00 63318328.00 54833988.00 137310.00 B 70220888.00 32809110.00 33408692.00 137996.00 C 32814770.00 18036550.00 28924962.00 157421.00 D 15809756.00 19806694.00 13356670.00 145957.00 E 7739579.00 8435984.00 9866096.00 147709.00 F 6266652.00 8248723.00 6727736.00 135730.00 G 5929060.00 6488747.00 4661878.00 138605.00 H 2274865.00 1607473.00 2161918.00 101930352.00 Load time: 09/30/2011 01:50:37 PM Read time: 09/30/2011 01:50:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1900 Comment: plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37872460 cps Min counts: 990 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15638978.00 16762386.00 15798102.00 17597838.00 19057252.00 15737798.00 27199604.00 20180964.00 13612760.00 22718778.00 26593356.00 33149450.00 B 13406412.00 11598722.00 11595172.00 12480585.00 13778280.00 12509688.00 17779574.00 19219240.00 17483768.00 15274984.00 21776144.00 19458712.00 C 13266307.00 9147378.00 7178668.00 7793972.00 11219907.00 10880857.00 17700902.00 15116824.00 19388874.00 22306824.00 21763810.00 20885834.00 D 10212640.00 11611292.00 6483499.00 9168678.00 13281237.00 16689906.00 37872460.00 17223352.00 21181992.00 20747788.00 21629060.00 21723386.00 E 10625026.00 6385400.00 2094.00 7925970.00 9088824.00 2037.00 27988332.00 22551882.00 2285.00 26751312.00 24175702.00 2075.00 F 9692257.00 6440546.00 2113.00 7154400.00 10094060.00 2094.00 21740460.00 31951586.00 2171.00 25776550.00 24470794.00 1980.00 G 11810098.00 8840612.00 2190.00 8390570.00 13884495.00 2228.00 20129044.00 23827660.00 2266.00 22937290.00 29362430.00 1999.00 H 14870237.00 9331710.00 2247.00 10465627.00 13440021.00 2132.00 20360690.00 10740984.00 2171.00 29802170.00 24063836.00 2056.00 Load time: 09/30/2011 01:52:24 PM Read time: 09/30/2011 01:52:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1901 Comment: plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 80816264 cps Min counts: 961 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 70743088.00 64991160.00 60579876.00 68364200.00 34390060.00 62756968.00 27573120.00 29430780.00 26001314.00 31112410.00 29583918.00 31392502.00 B 72628168.00 63571696.00 62237732.00 69333368.00 65140408.00 70893544.00 32173912.00 36094304.00 30478824.00 31627716.00 33991148.00 27689296.00 C 73146440.00 62646596.00 65907064.00 71397056.00 75764416.00 61910740.00 31051602.00 29081432.00 33478950.00 28870472.00 32123032.00 30616754.00 D 80709240.00 65899272.00 68826848.00 67074484.00 80816264.00 76820928.00 32868140.00 32834244.00 34304460.00 35772528.00 36223196.00 33247358.00 E 60310096.00 62382572.00 2171.00 63613168.00 68006008.00 2113.00 30477156.00 29654546.00 2342.00 18795608.00 30119886.00 2056.00 F 63488912.00 63931724.00 2209.00 61566248.00 66323416.00 2018.00 31563220.00 33090104.00 2152.00 33595304.00 35622404.00 2018.00 G 61761228.00 57805984.00 2037.00 60236512.00 63398608.00 2266.00 31061248.00 28226126.00 2113.00 31621308.00 31498602.00 1999.00 H 73484080.00 54960324.00 2037.00 47235884.00 57538644.00 2037.00 30656222.00 27125924.00 1923.00 32754144.00 32933638.00 1999.00 Load time: 09/30/2011 01:54:08 PM Read time: 09/30/2011 01:54:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1902 Comment: plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 50273444 cps Min counts: 980 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28582840.00 11054905.00 18372708.00 10378297.00 44369904.00 18684140.00 19789308.00 15951649.00 13010263.00 24667734.00 31580852.00 20766732.00 B 22201496.00 23302570.00 23070936.00 25027204.00 21282026.00 34345392.00 14771204.00 14597886.00 9736339.00 13249048.00 15053113.00 8837597.00 C 22452956.00 19908654.00 15613561.00 9517732.00 50273444.00 39020308.00 11082470.00 12111817.00 10458247.00 13940648.00 10108094.00 14708011.00 D 21870900.00 26239828.00 20702140.00 41110252.00 36933220.00 8262029.00 12146179.00 11974200.00 9878215.00 11471901.00 9575905.00 7831366.00 E 21482234.00 37603720.00 2113.00 47325580.00 46259664.00 2171.00 19261620.00 13215849.00 2113.00 28126996.00 7552915.00 2056.00 F 18703448.00 31335502.00 2228.00 36150172.00 27516610.00 2018.00 49157324.00 19382754.00 2152.00 34428460.00 8087592.00 1961.00 G 17651476.00 30315600.00 2132.00 24202704.00 21670942.00 2171.00 14461275.00 19711152.00 2075.00 4882619.00 9061089.00 2018.00 H 15455451.00 9069950.00 2190.00 27046166.00 7160823.00 2171.00 6732399.00 11932913.00 2132.00 4402009.00 10830807.00 2094.00 Load time: 09/30/2011 01:55:55 PM Read time: 09/30/2011 01:55:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1903 Comment: plate4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 80362512 cps Min counts: 895 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12822157.00 9021697.00 5997651.00 4449368.00 3624731.00 7777491.00 9157720.00 10199794.00 10700496.00 27007680.00 14487219.00 16592423.00 B 13815958.00 8867938.00 7241101.00 10877127.00 7985999.00 8883278.00 14997863.00 24262022.00 13756485.00 21442256.00 18276626.00 18661432.00 C 14977044.00 9850174.00 5935287.00 18243160.00 10385647.00 9604426.00 13946805.00 18312280.00 16148363.00 19423730.00 19918108.00 12875468.00 D 16674423.00 9183000.00 4381745.00 23905210.00 11000124.00 7896692.00 27348844.00 22343560.00 10756121.00 17246886.00 19113284.00 8107051.00 E 13371597.00 9870664.00 2094.00 25275182.00 14718417.00 1961.00 20506492.00 17275188.00 2266.00 19811772.00 14284850.00 2056.00 F 14381107.00 9290068.00 1866.00 80362512.00 10014016.00 2209.00 16665908.00 17268992.00 2285.00 13603478.00 12289351.00 2018.00 G 14719082.00 9759007.00 1999.00 14144052.00 10524963.00 2113.00 12419267.00 14513473.00 2152.00 8006697.00 11463707.00 1904.00 H 10611830.00 9271848.00 2209.00 5948008.00 7905535.00 1999.00 10911588.00 7268610.00 1790.00 4432915.00 5242121.00 1980.00 Load time: 09/30/2011 01:57:42 PM Read time: 09/30/2011 01:57:42 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1904 Comment: plate5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 71685752 cps Min counts: 971 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 63588276.00 58672960.00 54626252.00 53805604.00 45828088.00 58036936.00 46516484.00 26995532.00 14236282.00 15319952.00 17758244.00 19752576.00 B 71685752.00 55447872.00 58481616.00 62141552.00 50420320.00 61129140.00 51580544.00 35606144.00 22926976.00 14056251.00 34803572.00 38447196.00 C 65825872.00 65630364.00 65794220.00 62773128.00 28728314.00 50500944.00 54145464.00 34823460.00 14471282.00 19035254.00 18839868.00 21452636.00 D 65324784.00 60161720.00 63203920.00 61732392.00 57698660.00 57012968.00 62073804.00 26922578.00 22310522.00 22274974.00 32198612.00 27636716.00 E 65893704.00 66113752.00 2228.00 60368908.00 49167864.00 2266.00 60963844.00 42522056.00 2152.00 22537808.00 20137150.00 1942.00 F 62791324.00 63568584.00 2113.00 49255104.00 49309892.00 2018.00 68029136.00 35567500.00 2152.00 37108212.00 18281080.00 1999.00 G 46622692.00 51359796.00 1980.00 58907788.00 46432312.00 2113.00 47321564.00 24012056.00 2209.00 39207020.00 13063098.00 1999.00 H 39968380.00 46774488.00 2266.00 52079880.00 42231992.00 2285.00 50199356.00 20237560.00 2075.00 40740944.00 17927988.00 1961.00 Load time: 09/30/2011 01:59:29 PM Read time: 09/30/2011 01:59:29 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1905 Comment: plate6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27851234 cps Min counts: 952 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21588234.00 21377794.00 27851234.00 11094717.00 8694375.00 9376127.00 21317678.00 16804638.00 7195660.00 10798003.00 9473910.00 7171877.00 B 23134634.00 22935472.00 18012232.00 10737746.00 8997628.00 5902020.00 25284358.00 22177512.00 9280684.00 9455708.00 10500721.00 16592951.00 C 23879962.00 17175116.00 20186604.00 13012368.00 7789630.00 8855606.00 20203438.00 21679856.00 15198142.00 12865255.00 8972779.00 7433070.00 D 21891708.00 20508702.00 24207436.00 13624005.00 8725125.00 8796458.00 23419334.00 13001806.00 5282902.00 14503056.00 9334915.00 9067072.00 E 21765086.00 14085668.00 2266.00 14810044.00 7158234.00 1961.00 23989532.00 9934361.00 2685.00 11678567.00 8524280.00 1904.00 F 23950790.00 16623041.00 2190.00 11631081.00 6959708.00 1980.00 24471332.00 12761284.00 1980.00 13018189.00 7809469.00 2152.00 G 22508414.00 17780870.00 2056.00 9340772.00 7838386.00 1999.00 26904934.00 18550752.00 2075.00 11167158.00 7694263.00 2247.00 H 20817556.00 16057349.00 1980.00 8758679.00 7141013.00 1980.00 24390074.00 17200072.00 2132.00 20452188.00 8534343.00 2132.00 Load time: 09/30/2011 02:01:15 PM Read time: 09/30/2011 02:01:15 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1906 Comment: plate8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 48513184 cps Min counts: 990 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 48513184.00 15341072.00 9298860.00 21886672.00 16794796.00 20722324.00 25498666.00 28471940.00 24746774.00 25184602.00 35989064.00 36202016.00 B 34753952.00 15046987.00 13593096.00 24836040.00 12483602.00 26172038.00 32945740.00 32878394.00 28442458.00 35145724.00 37077344.00 12422115.00 C 14436170.00 19412130.00 16668438.00 28197558.00 22780146.00 22588518.00 33852192.00 27556756.00 33358114.00 36295836.00 36233460.00 33930676.00 D 14189024.00 21742882.00 19491046.00 46490428.00 20176160.00 23952996.00 31425512.00 27374660.00 31134142.00 35978932.00 35226828.00 37792096.00 E 14277137.00 17023316.00 2056.00 31960620.00 18277698.00 2056.00 32455152.00 30273482.00 2075.00 37753500.00 34207736.00 2132.00 F 15501644.00 25343584.00 2056.00 23028908.00 13764616.00 2285.00 32552394.00 47314968.00 2190.00 37275248.00 36055764.00 1980.00 G 11702553.00 21643464.00 2094.00 23762118.00 35031296.00 2171.00 33169322.00 29032182.00 2094.00 36671388.00 36849304.00 2056.00 H 15205251.00 15054652.00 2018.00 16912370.00 32627148.00 2171.00 30294646.00 27282652.00 1980.00 37385472.00 38719908.00 2075.00 Load time: 09/30/2011 02:19:44 PM Read time: 09/30/2011 02:19:44 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1907 Comment: standards final Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 214250016 cps Min counts: 59285 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 193271440.00 197744592.00 214250016.00 120894.00 145042.00 B 112121496.00 111814528.00 121355784.00 118571.00 146185.00 C 64541612.00 65738216.00 71968824.00 120551.00 171172.00 D 38735872.00 41440904.00 42941524.00 121256.00 147823.00 E 24285966.00 25415008.00 26174762.00 121370.00 147956.00 F 14846519.00 15110790.00 15279921.00 194749.00 149899.00 G 8643786.00 8946721.00 8967838.00 153384.00 125788.00 H 5306925.00 5312075.00 5349678.00 124874.00 471770.00