Load time: 10/6/2011 8:21 Read time: 10/6/2011 8:21 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1915 Comment: "standards , #449" Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 191226896 cps Min counts: 70196 counts Well list: "r0(a1:h3,a4:h4,a8:h11,a7:h7,a12:h12) = cps(f2(a1:h3,a4:h4,a8:h11,a7:h7,a12:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 191226896 172651264 143419 146237 37587016 31972430 32957334 34704228 34888392 32300102 B 106727208 98204944 144923 146865 33322120 35403544 31948192 33190918 33751816 34717364 C 60300036 55107156 146655 148959 34712264 38803808 33972396 16735406 35349292 38208456 D 35788668 33411792 145075 148845 39560204 37398084 33581304 34252964 38437684 34092564 E 22461218 20965718 146960 149359 33416516 36811380 34533004 46770660 39178752 48690372 F 14161829 12613279 144980 148749 35272220 34886360 36930288 36373152 35676008 42500776 G 8450188 7601270 140392 141991 34369224 38723064 33196144 34728804 32665316 49627200 H 5130941 4640018 144047 143952 32253466 37150940 36521372 36102344 38026776 37180876 Load time: 10/6/2011 8:23 Read time: 10/6/2011 8:23 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1916 Comment: #448 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22610042 cps Min counts: 779727 counts Well list: r0(a1:h9) = cps(f2(a1:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 20891624 17159462 16491389 17638622 18519482 18798220 19383432 17741404 16240639 B 20668836 16307789 16520808 16230902 18387460 19194808 19016080 16725283 12514468 C 22610042 16561865 15206661 16417774 17592220 19437114 19499140 17741162 14547918 D 18339930 15694084 15110356 19904722 21793556 21246970 19835954 18703798 17497002 E 21996812 15586977 16883434 18339238 18629658 19775236 21093844 17695986 16057557 F 19319986 13109426 15377757 14597678 16299097 19283484 19407894 18166706 14796322 G 15847092 12404609 11864118 13471614 15475792 18851076 17735070 15743042 12129534 H 20760460 16035003 14649266 15967326 16314170 17326896 18477132 16082131 12408814 Load time: 10/6/2011 8:25 Read time: 10/6/2011 8:25 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1917 Comment: wheat 9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 67553920 cps Min counts: 904 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8771106 67553920 7849428 8926353 8571638 7716126 16892982 18007444 17668678 15722565 14847543 11800609 B 7859571 11995773 8275009 8597260 8566769 7390979 19225808 16156207 18537670 14347206 15768097 18474682 C 7741929 7044406 8147505 7975905 7895512 8174591 16230293 17537576 15893607 14413590 13942481 10087947 D 8399006 9186592 8661033 8644670 7557353 8369283 15280808 12824679 13235869 10944959 5651916 13102377 E 7572318 6366870 1846 8497449 7059144 1922 16338272 17898648 2151 12203495 10891724 1808 F 8411855 7745367 2017 7259818 7863598 2093 14788828 17347792 2113 13740252 12143494 2151 G 8166646 8894238 1884 7770112 8213876 2074 7894809 14596420 2208 13037006 14098046 2074 H 8052766 8129572 2113 7824451 5071660 2017 15925192 13769246 2151 13001202 11562158 1960 Load time: 10/6/2011 8:27 Read time: 10/6/2011 8:27 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1918 Comment: wheat 10 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32580148 cps Min counts: 942 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14207296 12317602 5566179 20143680 18981356 19604102 11668884 19297850 16410412 14177769 15345072 7264349 B 13451418 12693917 14092109 16772845 17008354 14857389 16216082 17974150 27039986 15001542 19316110 17180294 C 14502621 4188665 14736227 16718039 15271589 16404475 16713488 22334598 22078594 16838226 17112052 17662156 D 11463632 7934439 9325559 16200449 14964201 14692798 19186542 12623039 14350112 14255244 6668505 12469915 E 11110824 13038358 2170 15101168 16399299 2036 5954683 32580148 2113 22171448 29481758 1884 F 13470009 10867677 2113 15111791 16911850 2227 17406228 24188328 2113 26055712 24224850 1941 G 16605567 14002975 2170 18350290 18659886 2265 6683698 15024667 2265 17316584 21963786 1960 H 16643244 17710536 1998 19924150 19303486 2113 13831433 12026877 1941 14518418 29295778 1998