Load time: 12/08/2011 04:27:16 PM Read time: 12/08/2011 04:27:16 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2047 Comment: Standards (23.5 highest), DNA Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 71172280 cps Min counts: 60609 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25834438.00 26686434.00 27331216.00 121219.00 29099058.00 B 16206454.00 17170354.00 17062440.00 121428.00 17045444.00 C 9910599.00 8557107.00 10703924.00 124051.00 32084998.00 D 6187702.00 6546242.00 6289051.00 214705.00 71172280.00 E 3745143.00 3883111.00 3941118.00 159883.00 124774.00 F 2383566.00 2508261.00 2527922.00 126123.00 124964.00 G 1488166.00 1556710.00 1522553.00 126199.00 128119.00 H 999296.00 994715.00 1021917.00 126256.00 128233.00 Load time: 12/08/2011 04:28:57 PM Read time: 12/08/2011 04:28:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2048 Comment: Plate#1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 11049475 cps Min counts: 70399 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 5323438.00 4746050.00 5246537.00 7152659.00 4123425.00 3126904.00 7407919.00 144562.00 5336090.00 6938293.00 7149447.00 8753399.00 B 2931778.00 6149455.00 5072926.00 5701803.00 3363233.00 8959619.00 5923871.00 4599897.00 7515455.00 8428223.00 5644307.00 612428.00 C 4697904.00 6879350.00 6403142.00 5469264.00 2388245.00 7897620.00 5441126.00 4617027.00 6311907.00 3956551.00 4916732.00 9075012.00 D 6403816.00 7913385.00 6945354.00 4918515.00 5011147.00 6012836.00 4982166.00 6419438.00 9945691.00 5330564.00 6167346.00 8647008.00 E 4967417.00 8404160.00 8581307.00 5897014.00 4888465.00 5533882.00 6377176.00 3578657.00 8175559.00 9561030.00 705381.00 8075693.00 F 6169003.00 6581796.00 7582136.00 6252120.00 4159348.00 5256962.00 5860030.00 4018563.00 6815727.00 4369647.00 5505105.00 11049475.00 G 6156072.00 5813948.00 5085594.00 4630834.00 3296364.00 5160069.00 5815699.00 4414527.00 4216083.00 144220.00 146254.00 4891465.00 H 5469161.00 4709256.00 140798.00 4235917.00 4076314.00 7495140.00 5879109.00 151386.00 7272807.00 3687585.00 7812323.00 3888332.00 Load time: 12/08/2011 04:30:46 PM Read time: 12/08/2011 04:30:46 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2049 Comment: Plate#2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 8616063 cps Min counts: 88914 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4322529.00 5713879.00 1982826.00 177828.00 1818117.00 1742129.00 2439749.00 4882258.00 2672299.00 3230179.00 2971450.00 2297731.00 B 5131611.00 4390980.00 3969863.00 3481070.00 4884751.00 3966769.00 5833883.00 4874390.00 3499623.00 4947468.00 4417871.00 4453091.00 C 4858219.00 5125351.00 3529922.00 4044648.00 4012117.00 4438831.00 4086089.00 5180280.00 4814974.00 4688481.00 4257443.00 5701443.00 D 4025710.00 4311748.00 5221524.00 5159584.00 5209520.00 4080070.00 3799961.00 3606133.00 4861030.00 5064970.00 4370326.00 5342879.00 E 7712114.00 3107646.00 4494625.00 5737238.00 4675541.00 4795336.00 4048913.00 3528793.00 3829806.00 4691874.00 4239113.00 4249005.00 F 5112062.00 386127.00 4357413.00 2960969.00 5051651.00 3574133.00 6643391.00 3334484.00 5541507.00 5501607.00 3130349.00 4665724.00 G 4180535.00 5292744.00 3748192.00 5756431.00 3312846.00 3111710.00 4924403.00 4757980.00 3222418.00 5277555.00 3100595.00 5045065.00 H 5501708.00 3716306.00 4716739.00 8616063.00 6605488.00 6105414.00 3110677.00 4324957.00 5114124.00 3772385.00 2979464.00 3707566.00 Load time: 12/08/2011 04:32:38 PM Read time: 12/08/2011 04:32:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2050 Comment: Plate#3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 12922985 cps Min counts: 71359 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6214564.00 5245402.00 6883905.00 7738458.00 6522660.00 4993349.00 3009459.00 3534014.00 3105577.00 3043689.00 4898013.00 6175364.00 B 5610796.00 6962583.00 5100486.00 2813847.00 6706155.00 5779926.00 4740188.00 4450711.00 7545791.00 5004628.00 7405960.00 4624920.00 C 8504438.00 11439755.00 12922985.00 11626031.00 8488213.00 7293251.00 6057964.00 6880845.00 142718.00 7859537.00 5066849.00 164464.00 D 6981656.00 7390832.00 7442844.00 8875219.00 1838989.00 8028942.00 4564378.00 5564322.00 7979252.00 7519331.00 8647046.00 4537734.00 E 6297424.00 5992636.00 6469923.00 8141610.00 4804062.00 6836529.00 9685555.00 6120018.00 7199498.00 6776752.00 7334756.00 5779746.00 F 4583897.00 6982163.00 8728562.00 6035545.00 6124192.00 10175933.00 8362386.00 8541064.00 150949.00 5482953.00 6108432.00 5546177.00 G 7383766.00 7597613.00 6662543.00 6508408.00 7410161.00 8395968.00 7468181.00 6277916.00 8331702.00 4980139.00 4079347.00 4819015.00 H 5197592.00 6151880.00 8602678.00 6036130.00 5610335.00 7006120.00 5455025.00 6526613.00 6054007.00 5675941.00 3105240.00 1722487.00 Load time: 12/08/2011 04:34:24 PM Read time: 12/08/2011 04:34:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2051 Comment: Plate#4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10642548 cps Min counts: 67082 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2924683.00 7253172.00 134164.00 2851592.00 3896781.00 3310571.00 4859507.00 2391048.00 1464500.00 5466734.00 4206693.00 6403378.00 B 2261205.00 3861176.00 4833095.00 4655560.00 3526628.00 4444915.00 2373024.00 240766.00 1129260.00 1471363.00 4309490.00 4472814.00 C 4101825.00 5454086.00 4141794.00 5236984.00 4438677.00 4964737.00 4159161.00 3836439.00 3331574.00 4048674.00 3857256.00 4791054.00 D 2376364.00 3758975.00 5753547.00 3016164.00 3039230.00 4116414.00 152451.00 4253754.00 2258994.00 4231046.00 4499848.00 4744015.00 E 2598443.00 4505885.00 381527.00 3606804.00 3028185.00 4287553.00 3850185.00 3806454.00 2503383.00 4667136.00 2670585.00 8071958.00 F 6648309.00 138992.00 3072238.00 4016848.00 4599647.00 4038788.00 3146537.00 4389196.00 143288.00 2562401.00 2975489.00 10642548.00 G 5534189.00 6552301.00 4143105.00 2210620.00 5363926.00 4910889.00 4480897.00 3574464.00 3333733.00 4842434.00 2356803.00 5465697.00 H 3856171.00 4991927.00 3211200.00 3267630.00 7206969.00 6230352.00 2616267.00 4187526.00 4722590.00 2184294.00 3002592.00 5260223.00 Load time: 12/08/2011 04:36:18 PM Read time: 12/08/2011 04:36:18 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2052 Comment: Plate#5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 7717441 cps Min counts: 81481 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3327123.00 2869784.00 2948979.00 6597233.00 4024634.00 1805881.00 3120657.00 3656642.00 3376399.00 5494530.00 5851301.00 3802294.00 B 3419760.00 4363167.00 2030682.00 4899958.00 1229475.00 2797637.00 162963.00 4183684.00 6964795.00 4406066.00 5592949.00 6870135.00 C 3213516.00 2196482.00 2367159.00 3233156.00 3595028.00 3950416.00 4600788.00 5608164.00 4141401.00 4247793.00 3850608.00 165054.00 D 3624438.00 2222910.00 6730968.00 5141696.00 4970607.00 3183676.00 2964169.00 271408.00 5073359.00 4716307.00 4582853.00 3366459.00 E 2076435.00 3114158.00 3280069.00 5135893.00 3677619.00 3253426.00 7717441.00 2727377.00 4783578.00 5237048.00 4975114.00 4417367.00 F 3108722.00 2496213.00 2635464.00 4256903.00 3394592.00 4178056.00 2760089.00 4699645.00 4970185.00 3667953.00 3123840.00 3886751.00 G 1139049.00 3010974.00 3428873.00 2911125.00 3090832.00 4029588.00 4444905.00 3805200.00 5875827.00 3554965.00 4436428.00 3244536.00 H 3319694.00 1885283.00 2949600.00 3405643.00 2401370.00 1975584.00 5415245.00 4025643.00 1965043.00 3314203.00 5565329.00 4325740.00 Load time: 12/08/2011 04:38:15 PM Read time: 12/08/2011 04:38:15 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2053 Comment: Plate#6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14899476 cps Min counts: 70779 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 141559.00 6993458.00 5115848.00 7982369.00 9881745.00 7872386.00 8296192.00 8044870.00 6675745.00 7625375.00 7274376.00 11599065.00 B 9124113.00 8846390.00 4423154.00 8868678.00 9971511.00 9690896.00 11552425.00 7407088.00 6717461.00 4700632.00 12585809.00 13391481.00 C 168304.00 8842751.00 144524.00 10565930.00 10040031.00 9605154.00 7702280.00 6757708.00 6086753.00 7317811.00 8717440.00 8780506.00 D 10103480.00 7858569.00 6873343.00 8585017.00 9359790.00 10686441.00 10654694.00 8122932.00 6197779.00 4658131.00 7402046.00 10345900.00 E 11163173.00 7742059.00 9445312.00 9492617.00 7191372.00 10283535.00 10067436.00 9364292.00 12685259.00 5462714.00 9225683.00 10728939.00 F 11628549.00 7516761.00 9529831.00 14899476.00 8110427.00 5277644.00 10974793.00 10654547.00 7339160.00 5948232.00 6783737.00 7350571.00 G 9727934.00 9479487.00 8809964.00 12511163.00 9488278.00 10263350.00 11066703.00 6860286.00 12002982.00 5965043.00 11303356.00 12837227.00 H 10237414.00 9654331.00 8045534.00 11295681.00 12341273.00 9983517.00 5937812.00 8297280.00 10659127.00 6069004.00 9171510.00 12148634.00