Load time: 02/02/2012 02:53:36 PM Read time: 02/02/2012 02:53:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2183 Comment: Standard and Nagu Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37532792 cps Min counts: 66358 counts Well list: r0(a1:h4,a7:h10) = cps(f2(a1:h4,a7:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36033572.00 37532792.00 37269620.00 134218.00 1388244.00 956484.00 4439100.00 804129.00 B 22934500.00 23421940.00 22027152.00 133343.00 1848677.00 944241.00 1185244.00 19742678.00 C 14482512.00 14666911.00 13957894.00 136347.00 1386381.00 1019410.00 1192982.00 6925856.00 D 8681582.00 9316066.00 8969544.00 136385.00 3294267.00 1996222.00 915249.00 2718089.00 E 5408269.00 5499398.00 5476424.00 134199.00 1028232.00 1013289.00 2550298.00 3643994.00 F 3323040.00 3292772.00 3094144.00 132716.00 1347465.00 1572365.00 2672817.00 2362036.00 G 2049526.00 2077660.00 1972056.00 135149.00 496378.00 881618.00 1445315.00 3399784.00 H 1299120.00 1308873.00 1280927.00 135472.00 1302162.00 2556900.00 1256649.00 3320881.00 Load time: 02/02/2012 02:55:21 PM Read time: 02/02/2012 02:55:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2184 Comment: Standard and Nagu Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 17958782 cps Min counts: 778692 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17105142.00 11406962.00 10318719.00 12050264.00 11842174.00 11986878.00 12971446.00 11887949.00 9113881.00 13315335.00 B 15298093.00 9810328.00 7154644.00 8497673.00 7919629.00 10891281.00 11080446.00 6369977.00 7404394.00 11441269.00 C 16528476.00 9617362.00 12168348.00 11456031.00 12299194.00 11193293.00 12076651.00 13187007.00 11303833.00 11311256.00 D 15292113.00 11656642.00 10191167.00 10552666.00 11225518.00 12097271.00 10087478.00 12889278.00 9052609.00 10702946.00 E 17958782.00 13157128.00 10999279.00 12462793.00 13305938.00 8404517.00 10818589.00 12722014.00 11329672.00 14546577.00 F 15995596.00 12205006.00 11466997.00 10852801.00 12934645.00 13576555.00 10550478.00 11705775.00 12254040.00 13762151.00 G 16565078.00 12189453.00 7312218.00 3917466.00 11856634.00 10178578.00 7646447.00 9566098.00 7781900.00 3028681.00 H 14465911.00 11651879.00 9881436.00 14147659.00 11459150.00 10299379.00 7205387.00 9585304.00 5796877.00 9746416.00