Load time: 03/21/2012 01:29:39 PM Read time: 03/21/2012 01:29:39 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2365 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 66624056 cps Min counts: 59624 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66624056.00 66301516.00 63424756.00 131063.00 12162137.00 B 37266840.00 37832964.00 38406896.00 131729.00 12694580.00 C 23196722.00 24034502.00 23153438.00 132700.00 12750372.00 D 15003904.00 15652798.00 15164578.00 134526.00 13134959.00 E 8741827.00 9868515.00 9774073.00 132243.00 13307585.00 F 5598952.00 6069601.00 5912201.00 137989.00 13283336.00 G 3628410.00 3697743.00 3612910.00 136486.00 120295.00 H 2212934.00 2223237.00 2166000.00 134659.00 119249.00 Load time: 03/21/2012 01:31:06 PM Read time: 03/21/2012 01:31:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2366 Comment: Plate 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 8912717 cps Min counts: 223478 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1416076.00 1770702.00 4399953.00 1943564.00 1557987.00 768290.00 3921405.00 2600295.00 2081423.00 3916417.00 5450915.00 3116745.00 B 2259210.00 1820311.00 2547575.00 2044973.00 1349907.00 1121073.00 3641073.00 3083092.00 1373365.00 3147133.00 8912717.00 3272264.00 C 446956.00 2424399.00 2267233.00 1306492.00 2990663.00 1137206.00 1380442.00 2111858.00 2636732.00 2553274.00 4696325.00 2168024.00 D 2160438.00 2321684.00 2030233.00 3006994.00 3719700.00 1024787.00 5221812.00 1168560.00 1904695.00 2432919.00 4019864.00 2333311.00 E 1922163.00 4074160.00 1929858.00 1277269.00 1672581.00 1802362.00 2267875.00 1592878.00 2289429.00 2983545.00 4229969.00 3333699.00 F 3465296.00 2992654.00 1421441.00 987992.00 6247445.00 1184446.00 1510346.00 1336456.00 3493156.00 2467188.00 5432542.00 3605581.00 G 2969409.00 5591885.00 790188.00 4192725.00 1631006.00 1948505.00 2752076.00 1248846.00 1796648.00 2001856.00 6037535.00 1744958.00 H 4812996.00 6694555.00 1530265.00 1429965.00 1564679.00 1902998.00 4504646.00 2284068.00 1394578.00 6187586.00 1885525.00 2716674.00 Load time: 03/21/2012 01:33:11 PM Read time: 03/21/2012 01:33:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2367 Comment: Plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28374174 cps Min counts: 779268 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3034992.00 4177092.00 11787039.00 2248396.00 2030301.00 2335710.00 4897491.00 6206634.00 1923412.00 5079495.00 2325051.00 3077113.00 B 4207269.00 5196597.00 2321013.00 3294130.00 1896164.00 5012999.00 3017361.00 4254442.00 2565599.00 2774925.00 2119120.00 4835814.00 C 2770381.00 3961554.00 3249859.00 3278899.00 6948879.00 2319131.00 5574161.00 3769568.00 5063129.00 3277023.00 2928699.00 2988106.00 D 2444916.00 6722942.00 1573140.00 2810716.00 4460302.00 6263182.00 6303046.00 16677544.00 2738940.00 2421695.00 3528647.00 2984066.00 E 2641541.00 2341523.00 4187246.00 2787024.00 7786445.00 6069563.00 5353794.00 3165214.00 5536770.00 2948153.00 6770076.00 2338757.00 F 4415020.00 5161035.00 5635278.00 3142854.00 3529665.00 8082078.00 4610054.00 4541675.00 3029320.00 2317904.00 2137765.00 3505567.00 G 2672875.00 3040422.00 3042728.00 4044902.00 3871780.00 4293896.00 4004523.00 6834266.00 4902671.00 2880764.00 9732642.00 2535292.00 H 4248613.00 2206725.00 28374174.00 3614279.00 4567225.00 6486993.00 6071805.00 4166414.00 8464511.00 3167793.00 5430338.00 2191460.00 Load time: 03/21/2012 01:35:05 PM Read time: 03/21/2012 01:35:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2368 Comment: Plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19826236 cps Min counts: 607319 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2001873.00 2613058.00 1371691.00 1963270.00 2511789.00 5914422.00 2487041.00 2021261.00 4009396.00 3042891.00 3267251.00 5436673.00 B 2018396.00 1409684.00 1395758.00 4953096.00 3317938.00 1900983.00 1855171.00 3295932.00 5274428.00 2353396.00 2006392.00 4446624.00 C 3146232.00 1347186.00 2015416.00 3123817.00 3356448.00 6312067.00 3357909.00 2181788.00 3118965.00 2789286.00 4130191.00 5549027.00 D 1502698.00 1554903.00 2504503.00 3417929.00 3383894.00 4748069.00 2624823.00 2055941.00 6289392.00 2686826.00 2547280.00 7736193.00 E 1674429.00 3204984.00 12872658.00 2084568.00 1861820.00 2619992.00 3586239.00 2535962.00 5368440.00 5413661.00 3075002.00 6272003.00 F 2659313.00 1878718.00 1943120.00 2752200.00 9619443.00 1779948.00 1694438.00 19826236.00 6755316.00 11093323.00 8242500.00 5997772.00 G 3053193.00 2171307.00 6272382.00 1758508.00 3164210.00 1505266.00 4296434.00 3887526.00 3399446.00 4601843.00 10366250.00 5140187.00 H 3563120.00 4419509.00 6362381.00 12098579.00 2876729.00 1214639.00 2712174.00 2194899.00 4079951.00 7794717.00 6409421.00 4165822.00 Load time: 03/21/2012 01:37:02 PM Read time: 03/21/2012 01:37:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2369 Comment: Plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31647084 cps Min counts: 691886 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2264205.00 2290220.00 4462470.00 7200465.00 7043603.00 4263214.00 4443838.00 3167372.00 2759312.00 3456612.00 3637875.00 1383772.00 B 3477036.00 4064112.00 4584953.00 4204579.00 7052692.00 6295565.00 3933193.00 6662684.00 2671248.00 4250541.00 3414942.00 2416388.00 C 2117969.00 6357378.00 7933938.00 11384572.00 5492125.00 8610785.00 5402780.00 3802141.00 2557799.00 5265451.00 3638975.00 3714958.00 D 5459060.00 3588840.00 5490356.00 5151284.00 9389419.00 11169919.00 7275252.00 3889870.00 3285463.00 3436550.00 3541830.00 2859598.00 E 31647084.00 5205355.00 4697500.00 11816244.00 6540267.00 10549285.00 4473568.00 5644360.00 4546500.00 5202419.00 5008830.00 4993449.00 F 10379905.00 4042693.00 18235664.00 3693431.00 9642726.00 5367808.00 5604259.00 2047127.00 6683163.00 3149095.00 2719007.00 4016505.00 G 10822676.00 10123707.00 6852873.00 4841643.00 19166842.00 5284694.00 4263454.00 2331877.00 2972535.00 5570579.00 2067417.00 4362129.00 H 16813816.00 14957366.00 8310440.00 3683456.00 9569546.00 9813225.00 2683927.00 7358015.00 4430014.00 3711886.00 2507763.00 6099813.00 Load time: 03/21/2012 01:38:52 PM Read time: 03/21/2012 01:38:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2370 Comment: Plate 5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26078096 cps Min counts: 67558 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4439948.00 1938195.00 3187467.00 9010110.00 3236412.00 5225496.00 3611855.00 8409548.00 7652955.00 8047200.00 3858329.00 1666772.00 B 5089890.00 12287945.00 3190850.00 6169988.00 2142727.00 3977879.00 5660510.00 14382156.00 8371874.00 2197588.00 3080682.00 5697034.00 C 3100526.00 3492501.00 3496358.00 4654535.00 4010005.00 8306976.00 4626272.00 5275474.00 7175094.00 5308826.00 2985615.00 2163925.00 D 5053126.00 7092000.00 3485105.00 8896020.00 4640651.00 5729050.00 4026003.00 8355205.00 5821547.00 6884622.00 3863886.00 2730974.00 E 2839457.00 13891493.00 6249044.00 2906073.00 4457808.00 4440163.00 3897674.00 6854368.00 135116.00 6652566.00 2312963.00 4085964.00 F 4785878.00 10986828.00 8547541.00 3991392.00 2936552.00 5916173.00 3039056.00 26078096.00 5214837.00 6463534.00 3671981.00 3753783.00 G 2587438.00 10169418.00 9549341.00 5476662.00 3394645.00 5008308.00 5981529.00 5220011.00 4104395.00 7424607.00 3669122.00 5197575.00 H 1961650.00 4671544.00 11132229.00 9654469.00 2507179.00 13903215.00 4817328.00 5959349.00 665041.00 2954722.00 3107541.00 4278111.00 Load time: 03/21/2012 01:40:40 PM Read time: 03/21/2012 01:40:40 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2371 Comment: Plate 6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46195292 cps Min counts: 779268 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4342815.00 5036792.00 12141898.00 16549437.00 11363952.00 7188841.00 5160926.00 3767799.00 4896506.00 3093348.00 5071611.00 3478283.00 B 4427952.00 14100013.00 10476149.00 13152163.00 12965806.00 3022935.00 4775443.00 4985852.00 4882697.00 6245923.00 6090355.00 4119138.00 C 5200350.00 10203158.00 15325928.00 11102413.00 12798010.00 8315982.00 5885156.00 5048713.00 7872486.00 3723280.00 6463218.00 4306353.00 D 4154885.00 11577390.00 9898776.00 15200992.00 14777229.00 4374827.00 4872979.00 4146852.00 6718403.00 2294302.00 3990660.00 8108129.00 E 18024724.00 2399034.00 10655421.00 13175801.00 13007164.00 5933593.00 5415101.00 5826893.00 17589832.00 24320670.00 8501968.00 8226498.00 F 8609824.00 13848291.00 10584088.00 7596940.00 9635740.00 4887775.00 4901715.00 8110940.00 46195292.00 24152948.00 7130893.00 6002502.00 G 7560081.00 11412383.00 12190926.00 9856980.00 7287376.00 7153700.00 5561796.00 3008878.00 20614254.00 7395584.00 10092998.00 5012000.00 H 3669761.00 8311512.00 6683857.00 8079421.00 9149843.00 8580152.00 4890327.00 3452432.00 9451646.00 6388367.00 8218490.00 7136313.00 Load time: 03/21/2012 01:42:22 PM Read time: 03/21/2012 01:42:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2372 Comment: Plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19329678 cps Min counts: 352611 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2781016.00 1673862.00 726150.00 1614791.00 2341028.00 7167649.00 9613177.00 1582110.00 2505171.00 705222.00 4478036.00 3648240.00 B 2158284.00 1462973.00 6667598.00 940924.00 2687270.00 2540118.00 7753257.00 3134045.00 2867971.00 1594046.00 3065521.00 19329678.00 C 4301364.00 2000987.00 5420763.00 1960692.00 3043452.00 2004477.00 5892277.00 2082711.00 3004606.00 2252139.00 2749134.00 8269573.00 D 2188389.00 3452497.00 8134222.00 3732603.00 4168665.00 7084405.00 1856890.00 2296774.00 1723587.00 2990912.00 4662611.00 8726487.00 E 2420666.00 4150895.00 1648652.00 3041607.00 1468776.00 1992469.00 3254695.00 2509527.00 1850804.00 1755344.00 3323576.00 6777318.00 F 1790356.00 3311726.00 9701779.00 3660394.00 2472884.00 7911810.00 3066401.00 2503835.00 3269668.00 1386131.00 7147650.00 2108246.00 G 1821688.00 1709017.00 2492056.00 1977366.00 8949587.00 4936929.00 2516294.00 1757756.00 5550565.00 2911858.00 4467197.00 8282845.00 H 1637082.00 4927814.00 2570436.00 2290895.00 4535201.00 8953844.00 1574732.00 4193921.00 1257616.00 4311166.00 2901514.00 8800051.00 Load time: 03/21/2012 01:44:25 PM Read time: 03/21/2012 01:44:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2373 Comment: Plate 8 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 17124628 cps Min counts: 385591 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8287143.00 7697465.00 2232491.00 6866006.00 5352478.00 6106778.00 5284966.00 1771395.00 2541829.00 1709778.00 16474529.00 3056398.00 B 8182669.00 2109486.00 7828310.00 4275839.00 2860730.00 771182.00 1556720.00 1265474.00 2122268.00 1431506.00 12002518.00 12065274.00 C 6717477.00 7552366.00 5744084.00 3220465.00 2120501.00 1329855.00 2795861.00 2472870.00 2317646.00 1872719.00 13473736.00 3901948.00 D 6580223.00 5498534.00 7777213.00 2280395.00 3532278.00 1372775.00 1952085.00 1862267.00 1824763.00 1591708.00 7089948.00 3026449.00 E 7241247.00 5261572.00 2711359.00 1727120.00 2644753.00 2209437.00 2389247.00 2083124.00 1154253.00 5033138.00 8147004.00 5982700.00 F 5519159.00 3116193.00 4914246.00 2840894.00 1753457.00 3361123.00 2883523.00 1413375.00 1439744.00 5765102.00 12115978.00 4634882.00 G 4429740.00 2791802.00 2274312.00 1717624.00 3092256.00 6002886.00 1450150.00 1310848.00 1135209.00 4322635.00 10022834.00 2359170.00 H 6415018.00 1875559.00 1697758.00 5597242.00 1651492.00 5029471.00 1357232.00 1188365.00 921899.00 6738708.00 17124628.00 2248025.00