Load time: 05/10/2012 11:06:13 AM Read time: 05/10/2012 11:06:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2562 Comment: 20120510_revtakashi Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 105730144 cps Min counts: 1180 counts Well list: r0(a1:h7) = cps(f2(a1:h7)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 102021056.00 36584452.00 25703484.00 108292.00 2307835.00 4070376.00 2530.00 B 105730144.00 32502726.00 13061756.00 110748.00 2439266.00 4102851.00 2511.00 C 42697880.00 17518654.00 10405313.00 112221.00 4083121.00 4129424.00 2568.00 D 42764684.00 8604241.00 9957837.00 141513.00 2629263.00 4138958.00 2474.00 E 13707502.00 10728462.00 12886531.00 2492.00 3638991.00 4308953.00 2492.00 F 2296646.00 2993020.00 8854575.00 2719.00 3942436.00 3610876.00 2455.00 G 2069274.00 2632315.00 3964800.00 2492.00 3490641.00 3810322.00 2398.00 H 1494683.00 2644190.00 3425629.00 2360.00 3442676.00 3906980.00 2474.00