Load time: 5/25/2012 14:27 Read time: 5/25/2012 14:27 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2598 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 51971824 cps Min counts: 68270 counts Well list: "r0(a1:h4,a5:h6,a7:d7) = cps(f2(a1:h4,a5:h6,a7:d7))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51971824 51713108 45976100 137220 16491120 28549212 16395375 B 33427078 27045144 27248040 149693 6150591 19313094 10099667 C 16815754 15879169 15977216 156543 18004078 8609386 11852960 D 12408616 10374957 10094838 139390 16153391 14555718 16315497 E 7853057 6427905 6104620 136541 17271756 20690450 F 4558855 3772570 3703449 140485 10136090 14374559 G 2350017 2316491 2303550 136711 14623492 8659778 H 1446233 1373584 1393171 141221 19295832 15209589 Load time: 5/25/2012 14:28 Read time: 5/25/2012 14:28 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2599 Comment: db count 2-hyb Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 12747969 cps Min counts: 73356 counts Well list: "r0(a1:h4,a5:e5,a6:h9,a10:e10) = cps(f2(a1:h4,a5:e5,a6:h9,a10:e10))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4437250 3983761 7344352 1968046 3545625 1492162 9615988 5064531 1838505 268667 B 10132742 3202703 4636403 274196 4016079 4740222 3012087 5085157 1092293 1242628 C 6102312 7199269 5549861 5362315 3329813 5055967 4117197 5118551 3536294 1155450 D 10235041 8005472 4757044 7864860 2151217 10164469 12073553 9746793 8784220 1017191 E 6527262 3375826 4731215 146712 4574960 5781948 9579055 9938513 593396 3574373 F 5582055 3280934 3495720 2290397 7875046 10644216 12747969 2734234 G 1321183 756751 5661846 2333307 1147808 2022941 10420234 674819 H 3001799 6850033 1535581 1302181 1169225 9874859 3341444 633041 Load time: 5/25/2012 14:30 Read time: 5/25/2012 14:30 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2600 Comment: db count 3-control Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19753124 cps Min counts: 86083 counts Well list: "r0(a1:h4,a5:e5,a6:h9,a10:e10) = cps(f2(a1:h4,a5:e5,a6:h9,a10:e10))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 178375 7011111 19229822 3945754 4450807 7512787 8954408 5457592 3497036 1771359 B 11511572 2370831 13593754 19753124 10028235 5309122 10889656 3194111 2194693 2721533 C 12502029 3966011 3577369 5452310 7723651 1914829 10756682 3820990 3590910 2658958 D 10872254 18670762 9383960 12389105 3078818 7311282 11634832 9324052 10942661 1780375 E 12836404 5314328 4232761 1512258 3936909 9454740 4182384 172167 2743442 5553179 F 12856026 1869031 7524276 2579169 10241930 2293201 3326081 1701150 G 11824906 1220834 3953300 2063376 3364218 2183956 2397839 1685916 H 3567753 7794556 1786491 1163357 1674091 8901637 1919765 1452969