Load time: 05/30/2012 01:27:14 PM Read time: 05/30/2012 01:27:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2620 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45560568 cps Min counts: 55841 counts Well list: r0(a1:h3,a4,a5,b5,b4,c4,c5,d4,d5,e4,f5,e5,f4,g4,g5,h4,h5,a6,b6,c6,d6:e6,f6,g6,h6,a7,b7,a8,b8,c8,d8,e8,f8,g8,h8,h9,g9,f9,e9,d9,b9,c9,a9) = cps(f2(a1:h3,a4,a5,b5,b4,c4,c5,d4,d5,e4,f5,e5,f4,g4,g5,h4,h5,a6,b6,c6,d6:e6,f6,g6,h6,a7,b7,a8,b8,c8,d8,e8,f8,g8,h8,h9,g9,f9,e9,d9,b9,c9,a9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44458752.00 45560568.00 44649884.00 119013.00 114574.00 5704867.00 6652286.00 116897.00 118598.00 B 33519518.00 30382836.00 28315720.00 118465.00 115556.00 6214904.00 5022285.00 116595.00 116935.00 C 18387822.00 17469334.00 16672690.00 121148.00 116274.00 2014843.00 111759.00 111683.00 D 10545900.00 11088679.00 8965871.00 122640.00 116331.00 2246895.00 222631.00 122130.00 E 5974769.00 6277914.00 5921071.00 120468.00 119863.00 1326077.00 119693.00 124208.00 F 3448734.00 3838233.00 3392229.00 119731.00 116028.00 1055727.00 123453.00 121545.00 G 2259007.00 2385745.00 2047791.00 122074.00 118673.00 1178084.00 121998.00 124341.00 H 1434814.00 1313533.00 1291960.00 123472.00 116501.00 115065.00 122621.00 124435.00 Load time: 05/30/2012 01:29:34 PM Read time: 05/30/2012 01:29:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2621 Comment: plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24566874 cps Min counts: 92585 counts Well list: r0(a1:h4,a5:e5,a6:h9,a10:e10) = cps(f2(a1:h4,a5:e5,a6:h9,a10:e10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24566874.00 2637945.00 6597781.00 1146272.00 703654.00 10633609.00 3614565.00 9066492.00 3972612.00 497156.00 B 14510713.00 3813581.00 3831141.00 1000395.00 1730012.00 3173941.00 727400.00 275677.00 185170.00 602606.00 C 7827411.00 4763135.00 4784629.00 11190980.00 693604.00 1795018.00 844940.00 980635.00 1099932.00 245149.00 D 13105084.00 6872949.00 3335694.00 4388591.00 3877857.00 14408924.00 16025123.00 4384593.00 16069487.00 650230.00 E 7090079.00 3314867.00 10439487.00 1656665.00 1519975.00 2312954.00 11785677.00 5175360.00 726002.00 5510210.00 F 5303202.00 4425302.00 17227568.00 2402123.00 2730766.00 15164237.00 7731069.00 3583210.00 G 1503483.00 2415414.00 4906589.00 797561.00 2416282.00 9336811.00 4766503.00 837422.00 H 3383512.00 8826682.00 1522298.00 424576.00 4319808.00 18926688.00 10171813.00 1520201.00 Load time: 05/30/2012 01:31:42 PM Read time: 05/30/2012 01:31:42 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2622 Comment: plate 5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 19285434 cps Min counts: 162699 counts Well list: r0(a1:h4,a5:e5,a6:h9,a10:e10) = cps(f2(a1:h4,a5:e5,a6:h9,a10:e10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10262171.00 9005387.00 12996678.00 5254345.00 13326501.00 7596358.00 1331423.00 2892095.00 626087.00 642938.00 B 13365864.00 7034440.00 11857866.00 10600759.00 17236126.00 9635033.00 731197.00 1923087.00 991478.00 647736.00 C 7218616.00 8492173.00 7999317.00 7872197.00 15941432.00 8809950.00 1375571.00 1029317.00 1055689.00 496324.00 D 9884695.00 10733037.00 12955549.00 11192940.00 8642985.00 10475328.00 2740989.00 1590911.00 1059373.00 566675.00 E 7131409.00 8280101.00 9171169.00 5190197.00 11395292.00 17761988.00 690033.00 1269536.00 375063.00 616491.00 F 7129773.00 8260636.00 6576674.00 8888094.00 17556854.00 4249673.00 1396238.00 755831.00 G 3900656.00 7454924.00 11272989.00 7510621.00 7890331.00 7552088.00 1052950.00 855973.00 H 5923296.00 12543229.00 1985882.00 3796755.00 7700439.00 19285434.00 325398.00 375535.00 Load time: 05/30/2012 01:33:21 PM Read time: 05/30/2012 01:33:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2623 Comment: plate 6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16029738 cps Min counts: 183488 counts Well list: r0(a1:h4,a5:e5,a6:h9,a10:e10) = cps(f2(a1:h4,a5:e5,a6:h9,a10:e10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16029738.00 4618001.00 2507905.00 1212390.00 8971618.00 10096729.00 9488421.00 11460881.00 2287289.00 964388.00 B 7163563.00 3387703.00 5961203.00 8885777.00 2480564.00 4073336.00 2438054.00 9842099.00 984866.00 1394406.00 C 7739126.00 852516.00 1943645.00 11649388.00 1188512.00 8025718.00 1077395.00 2780747.00 7892189.00 838593.00 D 8005756.00 4485102.00 7171091.00 15539801.00 770849.00 3631672.00 7896934.00 12015354.00 5843344.00 1895249.00 E 3958548.00 2119451.00 3292950.00 2036734.00 1464341.00 10705906.00 4181666.00 13188850.00 1995222.00 978519.00 F 1151372.00 1622423.00 6225244.00 906978.00 3703417.00 3364551.00 2821634.00 1000659.00 G 879416.00 504863.00 6184400.00 3154657.00 620817.00 4182959.00 16028278.00 921317.00 H 887540.00 4569976.00 1408744.00 1839065.00 1714284.00 6758098.00 1423725.00 366977.00