Load time: 06/08/2012 02:00:35 PM Read time: 06/08/2012 02:00:35 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2646 Comment: Standards, Whan, TILL, Takashi, gDNA Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24504054 cps Min counts: 1180 counts Well list: r0(a1:h7) = cps(f2(a1:h7)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24504054.00 20871282.00 21485482.00 122943.00 1634970.00 3333283.00 2920151.00 B 17102114.00 14553321.00 14045181.00 123566.00 3587420.00 1768424.00 3958166.00 C 8449009.00 8672561.00 8183693.00 123170.00 3397179.00 2699692.00 7430963.00 D 6162316.00 5359328.00 5208499.00 124095.00 2098190.00 1520950.00 2839007.00 E 3769305.00 3215722.00 3533450.00 127022.00 2133844.00 2201624.00 3864739.00 F 2464601.00 1967975.00 2069479.00 125530.00 1597610.00 1965665.00 13706813.00 G 1519496.00 1341030.00 1282032.00 126682.00 1459818.00 2950516.00 2360.00 H 995390.00 849595.00 888650.00 124888.00 1241221.00 2465001.00 2530.00 Load time: 06/08/2012 02:02:37 PM Read time: 06/08/2012 02:02:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2647 Comment: Plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 75183832 cps Min counts: 773544 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9403486.00 16913056.00 27646716.00 4007798.00 13781170.00 4082321.00 15737120.00 6202249.00 12458751.00 20748344.00 4018677.00 16032264.00 B 38346840.00 11002813.00 24808502.00 9058093.00 9437882.00 7197632.00 2574742.00 4559818.00 8041841.00 29963626.00 23430928.00 7266735.00 C 19347742.00 19141820.00 4468560.00 5600002.00 8914312.00 4586187.00 13935017.00 8465876.00 6096196.00 9830533.00 9380247.00 26295814.00 D 11282762.00 24246366.00 9731239.00 2514316.00 23444634.00 11736865.00 7231086.00 8051182.00 13311443.00 35370580.00 20587872.00 13661063.00 E 28270946.00 8356440.00 4148008.00 4320606.00 3836681.00 2057680.00 14978012.00 16439075.00 5854531.00 19059426.00 25207136.00 12171501.00 F 17302988.00 5289750.00 13829337.00 5897235.00 8442877.00 5033423.00 10669145.00 7523876.00 13151886.00 15664239.00 20254242.00 17968042.00 G 13180503.00 12386058.00 6588158.00 3319617.00 6168066.00 2613343.00 8973192.00 11461252.00 5193013.00 17497352.00 75183832.00 19121474.00 H 17102378.00 21566650.00 11753094.00 3695000.00 29157770.00 8971736.00 10038764.00 5739261.00 19094052.00 16332550.00 43203644.00 24382634.00 Load time: 06/08/2012 02:04:14 PM Read time: 06/08/2012 02:04:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2648 Comment: Plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 57514696 cps Min counts: 773544 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18708090.00 9321906.00 11238945.00 13163009.00 15308245.00 14595967.00 12153258.00 3916840.00 10273811.00 3793708.00 12373873.00 12790227.00 B 6850941.00 3232275.00 39832128.00 31097242.00 19167766.00 28778212.00 35342464.00 4968622.00 14526975.00 4396757.00 2987218.00 14483347.00 C 20020912.00 11113387.00 5278866.00 9693812.00 14400007.00 11714859.00 10147713.00 2846426.00 3341592.00 8285737.00 21262598.00 57514696.00 D 4824615.00 21705596.00 11012478.00 8875384.00 24232618.00 12512237.00 23724558.00 8671541.00 1793884.00 32890732.00 3204727.00 20362798.00 E 10960843.00 3400505.00 13974793.00 16144390.00 13039264.00 20994942.00 16840628.00 15908882.00 8831651.00 25154674.00 8293048.00 31551846.00 F 12761930.00 10076123.00 15484320.00 4846669.00 8045254.00 5891972.00 26118948.00 14234289.00 14261267.00 32929798.00 2593345.00 14734759.00 G 15357937.00 17367946.00 40474888.00 9179385.00 15333734.00 40960112.00 8704366.00 13031641.00 5041299.00 4412395.00 19079828.00 8447634.00 H 9714484.00 44749216.00 15292325.00 22230064.00 5659050.00 7610528.00 5980392.00 9349896.00 4469520.00 10418367.00 12066094.00 6539552.00 Load time: 06/08/2012 02:05:47 PM Read time: 06/08/2012 02:05:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2649 Comment: Plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 73497960 cps Min counts: 690391 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 5883573.00 4695678.00 10004150.00 3499225.00 9264043.00 13888057.00 11599900.00 20074168.00 10570082.00 36425036.00 25840702.00 18691092.00 B 7598447.00 4375461.00 1380783.00 9444278.00 2869345.00 10039962.00 20240992.00 6422970.00 3510141.00 20762992.00 4714621.00 14288717.00 C 23637272.00 11489085.00 5175163.00 5040727.00 13833987.00 5697748.00 8815376.00 7258437.00 7081168.00 12653273.00 31407088.00 4160198.00 D 42203512.00 12044563.00 5497668.00 12965678.00 9550031.00 19397228.00 9731741.00 3481156.00 15589453.00 7544055.00 47492232.00 13802318.00 E 45053904.00 26643978.00 6510501.00 32710338.00 7886280.00 25062012.00 14548449.00 8292896.00 4792847.00 15454096.00 4716262.00 3177321.00 F 46570976.00 22441860.00 22436134.00 14194797.00 23118676.00 24986400.00 57503152.00 11026825.00 73497960.00 28571470.00 10173686.00 16717576.00 G 11927026.00 20244222.00 28931482.00 4211759.00 14236281.00 14487224.00 5463283.00 2204532.00 9147924.00 26120536.00 18527688.00 15920276.00 H 16561167.00 8566265.00 11508537.00 2859197.00 5454848.00 10693127.00 3566316.00 4464916.00 4725800.00 20788268.00 5785219.00 2585919.00 Load time: 06/08/2012 02:10:52 PM Read time: 06/08/2012 02:10:52 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2650 Comment: Plate4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36052576 cps Min counts: 453985 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 25813194.00 8182931.00 12330676.00 966118.00 7362864.00 12140842.00 907970.00 15622511.00 3067017.00 19655694.00 18184988.00 7235482.00 B 10503457.00 19507976.00 28497158.00 3209840.00 15637164.00 7290789.00 4791831.00 1589248.00 2961260.00 2726796.00 7799544.00 4622928.00 C 28293592.00 19568972.00 3869120.00 5965352.00 5330709.00 11812970.00 3744418.00 4989016.00 7800850.00 29560230.00 7348616.00 2399434.00 D 16856958.00 5052996.00 9891689.00 3578097.00 8627564.00 3284726.00 17839336.00 1914855.00 7514447.00 5630500.00 12826024.00 8937672.00 E 6635305.00 14335612.00 6351931.00 12428685.00 1858337.00 11130689.00 3473855.00 4276367.00 6045938.00 33135604.00 20886498.00 6950518.00 F 9155581.00 2484482.00 22547044.00 7145625.00 11078800.00 10817859.00 6139442.00 4407469.00 6224744.00 8536712.00 12739042.00 26577610.00 G 14074850.00 2437032.00 2468660.00 12583905.00 11590462.00 10972846.00 1401538.00 9418600.00 7757089.00 12679615.00 21821690.00 36052576.00 H 4261429.00 8516683.00 8529662.00 8202115.00 22254430.00 6659532.00 4222627.00 12081849.00 1594603.00 3325433.00 14689486.00 9213770.00 Load time: 06/08/2012 02:14:02 PM Read time: 06/08/2012 02:14:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2651 Comment: Plate5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32627968 cps Min counts: 643490 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10817616.00 7575409.00 4687369.00 1569392.00 3504695.00 3711892.00 3337979.00 4393932.00 3751447.00 25373578.00 10683601.00 29723796.00 B 5386534.00 8361056.00 10536449.00 7044886.00 1862971.00 25980522.00 6351571.00 11255166.00 2890565.00 13428276.00 16322936.00 6340566.00 C 4899311.00 6844546.00 8811440.00 9913989.00 1790581.00 11857370.00 8545842.00 11980503.00 10190815.00 21879274.00 13072914.00 20854176.00 D 9510030.00 3924555.00 3138440.00 6215741.00 7032460.00 11227086.00 5546958.00 6167908.00 3081648.00 32627968.00 10083518.00 22883274.00 E 1971425.00 3071279.00 10043469.00 11030474.00 5477146.00 25447536.00 5216406.00 9028576.00 3753792.00 9977878.00 9956674.00 9889286.00 F 5186777.00 2548773.00 4500133.00 1702906.00 12445522.00 12721946.00 3326089.00 6390722.00 11934219.00 30737170.00 13405958.00 17405460.00 G 5263489.00 5695201.00 6719498.00 9246436.00 4862457.00 13160835.00 3423116.00 2577249.00 2113907.00 16910912.00 13934415.00 19335312.00 H 6209399.00 6909857.00 6321312.00 9835795.00 10105966.00 16154033.00 2542174.00 1286980.00 6744145.00 31498142.00 12283331.00 24095090.00 Load time: 06/08/2012 02:15:56 PM Read time: 06/08/2012 02:15:56 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2652 Comment: Plate6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38641840 cps Min counts: 58657 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38641840.00 22460430.00 5744372.00 12646757.00 1953059.00 4342415.00 14981232.00 6207729.00 121338.00 121791.00 119884.00 117315.00 B 8497822.00 11617687.00 9851302.00 6373872.00 4997783.00 10568449.00 1707502.00 7795370.00 121376.00 123850.00 120677.00 118656.00 C 10707336.00 6248003.00 5150151.00 3771494.00 12042443.00 8228709.00 5765887.00 6681367.00 123755.00 124133.00 122358.00 122509.00 D 4526751.00 11063146.00 22509776.00 9457904.00 4836605.00 1437307.00 2523333.00 4622180.00 124643.00 124548.00 124208.00 122358.00 E 12958271.00 7651684.00 11022332.00 4826216.00 3901906.00 5323548.00 8190260.00 10085516.00 121734.00 123944.00 120016.00 126569.00 F 11950998.00 5815014.00 1931882.00 3493817.00 948479.00 3479802.00 5289392.00 10404774.00 128080.00 125776.00 125039.00 121829.00 G 15903910.00 14759219.00 3520389.00 3417921.00 4730692.00 1628738.00 2334535.00 30726550.00 122773.00 128212.00 123604.00 119204.00 H 16732474.00 2031588.00 3062934.00 1756243.00 4215266.00 3988378.00 11224414.00 7833936.00 121791.00 169401.00 124416.00 122943.00 Load time: 06/08/2012 02:21:14 PM Read time: 06/08/2012 02:21:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2653 Comment: Standards 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 122150744 cps Min counts: 1114 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24812320.00 14654311.00 16050194.00 117391.00 1271286.00 2593699.00 2225017.00 122150744.00 2455.00 2228.00 2247.00 2341.00 B 16828722.00 12743114.00 9250462.00 117957.00 2837402.00 1373588.00 3057848.00 121758504.00 2304.00 2360.00 2455.00 2285.00 C 7067381.00 6097542.00 5753416.00 122811.00 1992119.00 1950431.00 7516287.00 120343504.00 2587.00 2379.00 2436.00 2247.00 D 6110282.00 3857601.00 3952581.00 121678.00 1599637.00 1113461.00 2051184.00 60145548.00 2436.00 2398.00 2285.00 2417.00 E 3646666.00 2347180.00 2680513.00 126645.00 1661468.00 1493755.00 2808024.00 61416748.00 2908.00 3059.00 2341.00 2247.00 F 2399124.00 1420915.00 1608023.00 121489.00 1271437.00 1479195.00 12789233.00 57443372.00 2643.00 2360.00 2228.00 2285.00 G 1440404.00 1083622.00 966023.00 117787.00 1171005.00 2005310.00 2492.00 2322.00 2511.00 2341.00 2285.00 2304.00 H 958242.00 695132.00 718776.00 118618.00 943795.00 1891251.00 2398.00 2341.00 2360.00 2417.00 2285.00 2360.00 Load time: 06/12/2012 03:10:25 PM Read time: 06/12/2012 03:10:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2655 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56038884 cps Min counts: 57601 counts Well list: r0(b1:h5,a1:a5) = cps(f2(b1:h5,a1:a5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56038884.00 55473160.00 50258848.00 115751.00 115203.00 B 34096464.00 30941152.00 32256426.00 118509.00 117961.00 C 17973890.00 17134848.00 17129042.00 122740.00 119925.00 D 9277801.00 9670903.00 9825192.00 121361.00 124534.00 E 5296553.00 5350374.00 5482709.00 126650.00 124402.00 F 2686678.00 2706371.00 2879643.00 123779.00 126574.00 G 1428965.00 1441016.00 1792866.00 123703.00 123250.00 H 924215.00 959613.00 1038644.00 131901.00 126064.00 Load time: 06/12/2012 03:13:05 PM Read time: 06/12/2012 03:13:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2656 Comment: Plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 11384989 cps Min counts: 55391 counts Well list: r0(a1:h4,a5:f5,a6:h9,a10:f10) = cps(f2(a1:h4,a5:f5,a6:h9,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8868907.00 1511150.00 8543865.00 281784.00 463609.00 2382281.00 833605.00 6533410.00 385806.00 512002.00 B 6566024.00 666476.00 2474114.00 187944.00 397422.00 4282026.00 323378.00 1299254.00 214445.00 321791.00 C 3687344.00 1132465.00 3117835.00 1323923.00 274191.00 2722398.00 882848.00 1781248.00 643394.00 331254.00 D 4311130.00 8684370.00 5341733.00 3118490.00 342323.00 1072285.00 4263657.00 2424858.00 833511.00 321942.00 E 11384989.00 4731995.00 3180121.00 420391.00 505448.00 3254066.00 703101.00 1469708.00 461191.00 330367.00 F 4096081.00 735156.00 3010184.00 414366.00 116941.00 2396505.00 500801.00 1968503.00 221038.00 110783.00 G 1785118.00 1908611.00 4466226.00 2668584.00 306699.00 482479.00 3370455.00 174287.00 H 979408.00 9880097.00 319411.00 333804.00 396251.00 4557450.00 320620.00 297953.00 Load time: 06/12/2012 03:15:20 PM Read time: 06/12/2012 03:15:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2657 Comment: Plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27060226 cps Min counts: 59367 counts Well list: r0(a1:h4,a5:f5,a6:h9,a10:f10) = cps(f2(a1:h4,a5:f5,a6:h9,a10:f10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17774080.00 10504382.00 23704672.00 403882.00 418710.00 12469775.00 5755995.00 19313802.00 368636.00 492339.00 B 24468520.00 7563784.00 11150371.00 253999.00 355697.00 13868513.00 4908582.00 4022571.00 214030.00 432121.00 C 11153925.00 6992345.00 8059780.00 7396347.00 928295.00 8623765.00 4396224.00 7311070.00 4909666.00 463685.00 D 17450756.00 27060226.00 14940739.00 12934884.00 787195.00 14814492.00 19630368.00 10138866.00 9110635.00 683004.00 E 23276142.00 15481721.00 13288136.00 575337.00 1553907.00 17877576.00 12967555.00 8784821.00 546342.00 1075270.00 F 16414718.00 9602654.00 9924901.00 368900.00 125139.00 14496662.00 5371514.00 7864818.00 546796.00 118735.00 G 2105251.00 14964233.00 14353052.00 222832.00 524828.00 11772999.00 280991.00 242986.00 H 2509699.00 23867748.00 2583833.00 350918.00 589258.00 20561380.00 1969462.00 354261.00