Load time: 6/12/2012 15:10 Read time: 6/12/2012 15:10 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2655 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56038884 cps Min counts: 57601 counts Well list: "r0(b1:h5,a1:a5) = cps(f2(b1:h5,a1:a5))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 56038884 55473160 50258848 115751 115203 B 34096464 30941152 32256426 118509 117961 C 17973890 17134848 17129042 122740 119925 D 9277801 9670903 9825192 121361 124534 E 5296553 5350374 5482709 126650 124402 F 2686678 2706371 2879643 123779 126574 G 1428965 1441016 1792866 123703 123250 H 924215 959613 1038644 131901 126064 Load time: 6/12/2012 15:13 Read time: 6/12/2012 15:13 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2656 Comment: Plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 11384989 cps Min counts: 55391 counts Well list: "r0(a1:h4,a5:f5,a6:h9,a10:f10) = cps(f2(a1:h4,a5:f5,a6:h9,a10:f10))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8868907 1511150 8543865 281784 463609 2382281 833605 6533410 385806 512002 B 6566024 666476 2474114 187944 397422 4282026 323378 1299254 214445 321791 C 3687344 1132465 3117835 1323923 274191 2722398 882848 1781248 643394 331254 D 4311130 8684370 5341733 3118490 342323 1072285 4263657 2424858 833511 321942 E 11384989 4731995 3180121 420391 505448 3254066 703101 1469708 461191 330367 F 4096081 735156 3010184 414366 116941 2396505 500801 1968503 221038 110783 G 1785118 1908611 4466226 2668584 306699 482479 3370455 174287 H 979408 9880097 319411 333804 396251 4557450 320620 297953 Load time: 6/12/2012 15:15 Read time: 6/12/2012 15:15 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2657 Comment: Plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27060226 cps Min counts: 59367 counts Well list: "r0(a1:h4,a5:f5,a6:h9,a10:f10) = cps(f2(a1:h4,a5:f5,a6:h9,a10:f10))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17774080 10504382 23704672 403882 418710 12469775 5755995 19313802 368636 492339 B 24468520 7563784 11150371 253999 355697 13868513 4908582 4022571 214030 432121 C 11153925 6992345 8059780 7396347 928295 8623765 4396224 7311070 4909666 463685 D 17450756 27060226 14940739 12934884 787195 14814492 19630368 10138866 9110635 683004 E 23276142 15481721 13288136 575337 1553907 17877576 12967555 8784821 546342 1075270 F 16414718 9602654 9924901 368900 125139 14496662 5371514 7864818 546796 118735 G 2105251 14964233 14353052 222832 524828 11772999 280991 242986 H 2509699 23867748 2583833 350918 589258 20561380 1969462 354261