Load time: 06/13/2012 03:45:08 PM Read time: 06/13/2012 03:45:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2659 Comment: Control Tech Rep 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53809820 cps Min counts: 48416 counts Well list: r0(a1:h6,a7:c7,a8:c11,d8:h11,a12:f12) = cps(f2(a1:h6,a7:c7,a8:c11,d8:h11,a12:f12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53809820.00 47863560.00 46606752.00 110986.00 4121863.00 660119.00 292254.00 8083248.00 2611445.00 13818227.00 238698.00 216663.00 B 26450464.00 25895378.00 26371972.00 110495.00 1275545.00 844864.00 899422.00 12670574.00 3031322.00 3134705.00 181324.00 239454.00 C 15800896.00 14879821.00 14878592.00 113046.00 4074540.00 3862981.00 995310.00 7830207.00 2368173.00 5397228.00 2955166.00 279045.00 D 9337824.00 9488680.00 9055871.00 113632.00 4169573.00 466586.00 10194127.00 16795572.00 9284421.00 6417982.00 474467.00 E 5491971.00 5552915.00 5586665.00 109002.00 2963858.00 522921.00 16252733.00 12084477.00 9064558.00 410120.00 2864314.00 F 3035693.00 2980085.00 2988609.00 111875.00 4481142.00 232764.00 10251478.00 5247442.00 8803654.00 165185.00 96832.00 G 1650456.00 1740416.00 1779188.00 109399.00 8856852.00 220216.00 1414236.00 8762147.00 12722869.00 254629.00 H 922326.00 951618.00 1027625.00 109267.00 4904102.00 225828.00 459689.00 17387172.00 2253929.00 235126.00 Load time: 06/13/2012 03:46:57 PM Read time: 06/13/2012 03:46:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2660 Comment: Control Tech Rep 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55147784 cps Min counts: 49436 counts Well list: r0(a1:h6,a7:c7,a8:c11,d8:h11,a12:f12) = cps(f2(a1:h6,a7:c7,a8:c11,d8:h11,a12:f12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 55147784.00 49072460.00 47815660.00 112801.00 4160772.00 504571.00 291139.00 8241168.00 2619381.00 14065199.00 234540.00 212846.00 B 27204758.00 25982822.00 26514522.00 110571.00 1282707.00 863403.00 856543.00 12765441.00 3028184.00 3171707.00 239775.00 235542.00 C 15843262.00 15417412.00 15067512.00 114860.00 4115289.00 3899961.00 1009615.00 7863216.00 2375303.00 5385752.00 2966104.00 280689.00 D 9423827.00 9675811.00 9289870.00 113197.00 4160363.00 468835.00 10210949.00 16839714.00 9444108.00 6414908.00 475639.00 E 5577170.00 5625198.00 5650691.00 111572.00 3017461.00 527135.00 16301094.00 12014625.00 9150715.00 413276.00 2899573.00 F 3106974.00 3031034.00 3036330.00 111988.00 4557866.00 226830.00 10381087.00 5325565.00 8799842.00 164373.00 98873.00 G 1677551.00 1770154.00 1797779.00 111912.00 8906819.00 221973.00 1410948.00 8884064.00 12843153.00 257048.00 H 939013.00 963145.00 1041723.00 110325.00 4986920.00 223069.00 464470.00 17581074.00 2279230.00 244178.00