Load time: 06/15/2012 09:43:01 AM Read time: 06/15/2012 09:43:01 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2664 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24458578 cps Min counts: 48504 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24458578.00 7228603.00 5547155.00 97443.00 9206041.00 6097875.00 3991228.00 5083224.00 2628764.00 5503152.00 4136190.00 3807230.00 B 3749408.00 3363130.00 2190510.00 98123.00 8766702.00 7012382.00 8234439.00 6112978.00 4382760.00 5591213.00 4263812.00 4325174.00 C 1843715.00 2455635.00 2533215.00 98312.00 7066165.00 6808817.00 7844013.00 7313621.00 4759657.00 5113284.00 4215640.00 3702019.00 D 548405.00 686767.00 579916.00 97008.00 8596183.00 6436819.00 6678556.00 8132560.00 5716632.00 5189029.00 4992821.00 3487357.00 E 404129.00 503046.00 362945.00 6114654.00 9569473.00 6214405.00 8089159.00 8559451.00 6169166.00 4735661.00 5457596.00 3804660.00 F 179319.00 191504.00 188897.00 5432817.00 9872315.00 6838640.00 7132824.00 10161309.00 5936646.00 4871467.00 5233562.00 4577490.00 G 142877.00 138022.00 143104.00 6067242.00 9669370.00 8141861.00 4858806.00 9709298.00 5615938.00 4930948.00 4473018.00 7053650.00 H 101013.00 107398.00 108834.00 7642017.00 9665649.00 6316309.00 6812089.00 9617439.00 5927080.00 6451502.00 4733254.00 6584912.00