Load time: 06/20/2012 02:09:28 PM Read time: 06/20/2012 02:09:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2685 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 57944636 cps Min counts: 66609 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57944636.00 42941040.00 43772280.00 133634.00 B 34051020.00 25717770.00 24738880.00 135030.00 C 17823436.00 15640059.00 19448694.00 140240.00 D 12533433.00 10217723.00 10049718.00 136956.00 E 7729826.00 6586135.00 6410798.00 134426.00 F 4192318.00 3887180.00 3713181.00 133218.00 G 2654406.00 2166929.00 2260136.00 141863.00 H 1520106.00 1372071.00 1420485.00 137484.00 Load time: 06/20/2012 02:11:18 PM Read time: 06/20/2012 02:11:18 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2686 Comment: Plate 472 (1) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25092900 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13765822.00 14545439.00 10950944.00 14195881.00 14774427.00 20671020.00 21686734.00 16810680.00 14204044.00 14169369.00 B 14245343.00 13006225.00 14383450.00 9344109.00 16770459.00 22143214.00 19017644.00 17700446.00 6676616.00 12641415.00 C 15696327.00 16296307.00 10410693.00 11784621.00 17590658.00 24874032.00 18795668.00 12503312.00 7440778.00 10978781.00 D 16050180.00 14543467.00 20548884.00 16769802.00 16952264.00 20998168.00 19164158.00 22465972.00 13341733.00 12852815.00 E 17913050.00 19976454.00 14522188.00 15692441.00 17378440.00 22607480.00 22454552.00 14293092.00 16286009.00 12279088.00 F 15896690.00 15537934.00 17654368.00 9713400.00 12696846.00 20888500.00 20972194.00 17268358.00 6965864.00 7306128.00 G 17342928.00 17719106.00 15072254.00 11909134.00 14512700.00 25092900.00 18925048.00 13379554.00 6683975.00 5850113.00 H 17843512.00 18748130.00 13541537.00 16841294.00 8798162.00 22059496.00 17200816.00 11344144.00 10170395.00 7020816.00 Load time: 06/20/2012 02:12:50 PM Read time: 06/20/2012 02:12:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2687 Comment: Plate 476 (2) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23928258 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17725730.00 18618056.00 16725217.00 19500446.00 22029262.00 23361530.00 21459316.00 17711720.00 15431710.00 15833657.00 B 17053074.00 14222807.00 16373584.00 10369056.00 20331322.00 22100230.00 19550006.00 17866108.00 8149554.00 13280177.00 C 17623260.00 20896180.00 9286988.00 10709785.00 21097874.00 23928258.00 18003654.00 13053858.00 7489615.00 12137496.00 D 14764862.00 10818831.00 16539443.00 14595278.00 18991714.00 20798030.00 18395800.00 23405804.00 14022874.00 13260086.00 E 15690944.00 16799682.00 10434946.00 12318041.00 17827670.00 21445328.00 21164818.00 13887308.00 17331342.00 12747110.00 F 13729788.00 10110662.00 14402206.00 6050647.00 13538170.00 21602262.00 20439524.00 16156980.00 7312112.00 8369833.00 G 14001187.00 12293226.00 8518870.00 7989125.00 14539556.00 22087480.00 18881694.00 13114487.00 7607585.00 5724562.00 H 16443476.00 15119624.00 9706071.00 14882674.00 15556130.00 22506850.00 17549330.00 12333644.00 12187531.00 9184230.00 Load time: 06/20/2012 02:14:19 PM Read time: 06/20/2012 02:14:19 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2688 Comment: Plate 473 (3) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25853136 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10983757.00 12602673.00 8835496.00 11384938.00 14112003.00 19101800.00 20491316.00 16017195.00 14429625.00 14984529.00 B 10907560.00 9690255.00 9368624.00 5322784.00 13671034.00 21566768.00 18664578.00 17791728.00 6178536.00 11802936.00 C 10450223.00 13528976.00 4406001.00 4784283.00 12913656.00 25853136.00 18188794.00 12005820.00 7711052.00 10842972.00 D 9718430.00 6240140.00 11767293.00 8757719.00 12495592.00 21490334.00 20324054.00 23818708.00 14352022.00 12914412.00 E 9849864.00 11431890.00 5942055.00 9883080.00 12209916.00 23669654.00 21817028.00 14921308.00 17595264.00 12764241.00 F 7510525.00 5684166.00 8524967.00 2372047.00 6916619.00 22386932.00 21735450.00 17885660.00 5641460.00 8379431.00 G 8726983.00 6985577.00 3960371.00 3333855.00 7222941.00 9997734.00 19526848.00 15462450.00 5995680.00 6249548.00 H 5647790.00 10136379.00 4459064.00 7772013.00 8366164.00 23519376.00 19920864.00 13397802.00 13515212.00 9553450.00 Load time: 06/20/2012 02:15:50 PM Read time: 06/20/2012 02:15:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2689 Comment: Plate 465 (4) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37876428 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10700950.00 12426821.00 7515644.00 10495007.00 10282341.00 24262420.00 27916426.00 23814746.00 22938164.00 31141334.00 B 12169420.00 10181632.00 10883621.00 6171779.00 13096802.00 24750444.00 21660364.00 23573806.00 17507246.00 31128542.00 C 12544903.00 13327452.00 6333679.00 8240036.00 15376892.00 29500206.00 32145208.00 17519506.00 17882680.00 34825460.00 D 12577598.00 11062892.00 16020415.00 14211537.00 15772509.00 31537042.00 26392564.00 36072580.00 26643788.00 33216674.00 E 15600200.00 15589914.00 11218739.00 15487013.00 16702958.00 33449634.00 37859924.00 27030000.00 28158012.00 31726118.00 F 14419909.00 11310189.00 14032624.00 8211190.00 11419666.00 29966850.00 28395360.00 31986012.00 13952370.00 24537406.00 G 14266031.00 13755634.00 11390390.00 9163612.00 12822225.00 29652612.00 31562662.00 23936554.00 15934432.00 26164974.00 H 16137488.00 14691436.00 11516457.00 13894322.00 14736943.00 37876428.00 35314624.00 29170292.00 27140358.00 23116490.00 Load time: 06/20/2012 02:17:15 PM Read time: 06/20/2012 02:17:15 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2690 Comment: Plate 466 (5) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23585528 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7713938.00 9335151.00 5912358.00 8116272.00 9396691.00 19377234.00 17780316.00 13396826.00 8167746.00 9202487.00 B 8149356.00 7565553.00 8610619.00 6515400.00 11220384.00 21995356.00 18250150.00 15205834.00 19524826.00 7166220.00 C 8596698.00 9574330.00 5892468.00 7473029.00 12344515.00 23585528.00 17471914.00 9927625.00 20460946.00 5986826.00 D 9252587.00 8986812.00 12356353.00 11169790.00 11800522.00 20214158.00 18193162.00 20818696.00 7158178.00 9783538.00 E 9956891.00 11779020.00 8946016.00 9881955.00 11945513.00 20897594.00 21673968.00 12670273.00 10730581.00 7113916.00 F 9842415.00 8957875.00 10843490.00 6476299.00 8867724.00 19289628.00 20222512.00 14436523.00 18505972.00 19595592.00 G 9951367.00 9738028.00 8005446.00 6832159.00 10282518.00 22973336.00 18687540.00 11937876.00 19224442.00 20391542.00 H 10573102.00 10461620.00 7658423.00 10215387.00 10338219.00 21384290.00 15921012.00 8542185.00 7877319.00 19201236.00 Load time: 06/20/2012 02:18:47 PM Read time: 06/20/2012 02:18:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2691 Comment: Plate 467 (6) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32122900 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11784118.00 13606672.00 10216683.00 14249939.00 15099721.00 22265144.00 24802612.00 18740266.00 24400330.00 25745346.00 B 13006510.00 10524224.00 10118549.00 8382055.00 13831382.00 24507226.00 20400584.00 23400136.00 15649369.00 27349756.00 C 11753983.00 13626721.00 6065385.00 7371865.00 14272959.00 26325344.00 26223460.00 14358846.00 16950516.00 28319326.00 D 10282190.00 7118868.00 10925286.00 11201234.00 14154711.00 25712724.00 22347910.00 31384434.00 26644888.00 29508084.00 E 11180548.00 9765890.00 7716679.00 11021744.00 13493912.00 27419600.00 31799718.00 22006688.00 26929634.00 28465826.00 F 10240310.00 3500262.00 9661651.00 6603438.00 10433863.00 26066260.00 26105336.00 30534238.00 18391818.00 25909280.00 G 9722378.00 6756189.00 5611560.00 4922316.00 8762631.00 31150870.00 32122900.00 23419770.00 17614026.00 29732668.00 H 11367379.00 9843830.00 7073205.00 9389160.00 11476812.00 30094230.00 29370428.00 21034788.00 26014596.00 25550924.00 Load time: 06/20/2012 02:20:19 PM Read time: 06/20/2012 02:20:19 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2692 Comment: Plate 475 (7) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32080916 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 18321426.00 16387848.00 13464394.00 14831027.00 16415930.00 21751350.00 23661684.00 16752397.00 9236559.00 12149474.00 B 18551756.00 14786775.00 15807273.00 8242944.00 17184912.00 26346244.00 21642600.00 17684494.00 17060148.00 9280421.00 C 19871810.00 17319654.00 10532525.00 10057903.00 18917686.00 28746630.00 23206274.00 11578930.00 18821980.00 8347258.00 D 19937200.00 14737167.00 20838448.00 14995601.00 17871272.00 25539784.00 25276754.00 24417208.00 9399721.00 12201417.00 E 20076106.00 23066898.00 15359815.00 16787172.00 18587664.00 28978618.00 28468656.00 14261637.00 15268929.00 11844164.00 F 19971190.00 17939402.00 19240614.00 9225503.00 15141596.00 28486806.00 27297708.00 21926178.00 5802226.00 7567330.00 G 22885610.00 17391422.00 15103260.00 12718527.00 16153977.00 31315152.00 28271584.00 19370338.00 9661543.00 6805042.00 H 23032880.00 20185712.00 15280428.00 14993218.00 17520100.00 32080916.00 28488696.00 19720304.00 16619297.00 11926550.00 Load time: 06/20/2012 02:21:48 PM Read time: 06/20/2012 02:21:48 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2693 Comment: Plate 469 (8) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18827894 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12145389.00 13966358.00 8951143.00 12383825.00 14929233.00 13194863.00 12571912.00 11602094.00 9316308.00 10182571.00 B 12392916.00 10000139.00 11800468.00 8559975.00 15309233.00 13943411.00 12529006.00 11732864.00 6303057.00 9990835.00 C 15126990.00 16371953.00 9002247.00 10711032.00 17803448.00 14246367.00 13032008.00 10285267.00 8178157.00 9875278.00 D 17046646.00 13015597.00 18150024.00 15729060.00 18214078.00 13378211.00 14043993.00 14917565.00 10962840.00 11000354.00 E 17204106.00 18545394.00 14041595.00 15357526.00 18119650.00 14803113.00 14542373.00 12137132.00 12799828.00 8380284.00 F 16166372.00 13854310.00 16814556.00 9439936.00 13222034.00 13695009.00 14409562.00 13869644.00 7408997.00 7472408.00 G 17600990.00 15731236.00 11976787.00 10903914.00 14140369.00 14696713.00 13398173.00 11445329.00 7674540.00 4939399.00 H 18827894.00 17768544.00 12118717.00 14636808.00 15209992.00 14496210.00 13817317.00 10418859.00 10128438.00 6959161.00 Load time: 06/20/2012 02:23:19 PM Read time: 06/20/2012 02:23:19 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2694 Comment: Plate 470 (9) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22593474 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12638480.00 14293937.00 9063595.00 4952311.00 19894976.00 16747027.00 18735954.00 16208061.00 13304653.00 13731885.00 B 13930146.00 11959593.00 7666237.00 20544732.00 21917472.00 19041858.00 17490098.00 15646423.00 5382136.00 10631142.00 C 13911697.00 12525737.00 5570593.00 21790770.00 22593474.00 18916946.00 16846834.00 10973342.00 6401652.00 9930354.00 D 13929518.00 14101835.00 17827960.00 22311792.00 21697264.00 17433228.00 16604805.00 19960104.00 13897593.00 12536074.00 E 17713990.00 17865076.00 9694107.00 12437636.00 8095790.00 15681300.00 19515314.00 12338624.00 15359998.00 12496783.00 F 17739556.00 16370810.00 13303441.00 21296928.00 20557846.00 16740174.00 18891244.00 14217339.00 6266046.00 7562097.00 G 20088260.00 19684000.00 13440403.00 20698802.00 21429396.00 18042266.00 15742032.00 12675882.00 5175778.00 6069148.00 H 20513606.00 16217817.00 10244110.00 11262918.00 8798413.00 18421398.00 16481126.00 11732596.00 11676671.00 9894083.00 Load time: 06/20/2012 02:24:47 PM Read time: 06/20/2012 02:24:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2695 Comment: Plate 471 (10) Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 25 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15786905 cps Min counts: 773114 counts Well list: r0(a1:h4,a5:h5,a7:h11) = cps(f2(a1:h4,a5:h5,a7:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11638912.00 11333484.00 9219134.00 11925152.00 13045664.00 12028703.00 11965479.00 9676792.00 8466367.00 8393267.00 B 9455072.00 10299641.00 10521101.00 8462975.00 12682163.00 13609594.00 11915797.00 10164979.00 4211773.00 6250448.00 C 10694717.00 12019949.00 7895806.00 8566207.00 13695955.00 14723246.00 12462739.00 7803092.00 4984724.00 7353298.00 D 12302556.00 10468434.00 13650974.00 12497389.00 13546923.00 13641436.00 13523014.00 14599466.00 9024409.00 9798082.00 E 12970048.00 11856170.00 11490134.00 13234075.00 14481059.00 15519189.00 14720443.00 10396609.00 11801549.00 9392263.00 F 12964240.00 11882208.00 13311480.00 9469629.00 12290352.00 15179501.00 14562424.00 12531441.00 6858791.00 6903963.00 G 12990426.00 11032078.00 9966981.00 9487479.00 11590144.00 15511776.00 13491390.00 11471720.00 7058198.00 6770780.00 H 13993179.00 14375052.00 11406978.00 12523159.00 12586956.00 15786905.00 13707173.00 11039135.00 10364330.00 9469907.00