Load time: 08/23/2012 10:07:34 AM Read time: 08/23/2012 10:07:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2807 Comment: Standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 51037132 cps Min counts: 57447 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49876416.00 47048920.00 51037132.00 115141.00 B 32013174.00 34171332.00 32512646.00 116256.00 C 20820764.00 21104390.00 20629460.00 114895.00 D 12604803.00 12453058.00 11996073.00 118335.00 E 7644653.00 7505058.00 7302069.00 116124.00 F 4436332.00 4233635.00 4280985.00 116124.00 G 2584793.00 2717051.00 2574944.00 117919.00 H 1588099.00 1546857.00 1610871.00 117220.00 Load time: 08/23/2012 10:09:13 AM Read time: 08/23/2012 10:09:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2808 Comment: Plate 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 122057472 cps Min counts: 55302 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8536305.00 8429654.00 18824464.00 2526802.00 9226031.00 111418.00 113024.00 7396716.00 92528928.00 16013739.00 14532382.00 46242080.00 B 1081634.00 8453467.00 24445568.00 6669921.00 4123514.00 112363.00 114555.00 20769876.00 40717708.00 26961032.00 42601432.00 18986186.00 C 5554970.00 5884195.00 19243156.00 3956422.00 9698614.00 111701.00 113780.00 122057472.00 37659828.00 28944524.00 33019770.00 29580560.00 D 3364874.00 6150697.00 14520607.00 7268552.00 4217640.00 112627.00 111096.00 45258852.00 35925480.00 31894866.00 68984848.00 20935400.00 E 2741287.00 8963151.00 7907743.00 4412566.00 2290738.00 110832.00 114933.00 49365636.00 29356338.00 18022328.00 5250605.00 41611536.00 F 3445757.00 18846278.00 27452544.00 7172547.00 3656173.00 110605.00 113591.00 15886075.00 16391417.00 35907816.00 10200109.00 55592304.00 G 2645748.00 5896618.00 17262288.00 5022019.00 2516106.00 112041.00 113327.00 25718636.00 53247976.00 38400120.00 19788956.00 61836972.00 H 2590173.00 11458623.00 24857176.00 5642200.00 8983788.00 113837.00 119620.00 36551892.00 46628944.00 14756140.00 8462792.00 70572096.00 Load time: 08/23/2012 10:11:22 AM Read time: 08/23/2012 10:11:22 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2809 Comment: Plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 72000984 cps Min counts: 774161 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 69209344.00 27601516.00 21718116.00 44719248.00 31559282.00 19383410.00 28763744.00 36916708.00 27607322.00 13520092.00 14094145.00 33077758.00 B 29981402.00 29871732.00 22952274.00 25892132.00 20968630.00 21151846.00 35362448.00 20935400.00 23033674.00 21620646.00 23930220.00 26115208.00 C 72000984.00 20451956.00 15579987.00 14011445.00 15510472.00 21234520.00 7066144.00 19297792.00 22924678.00 18459586.00 21852310.00 13801495.00 D 38499424.00 18984652.00 19978524.00 14315969.00 14215263.00 29467070.00 16096211.00 14469556.00 23655066.00 12450114.00 27298534.00 30791452.00 E 33109306.00 23280400.00 27514700.00 17035550.00 11332403.00 25771374.00 13872748.00 27811132.00 31106076.00 23012176.00 25147290.00 14307475.00 F 20517216.00 19771722.00 26814326.00 22342268.00 10460953.00 29824664.00 18330296.00 20188434.00 26750766.00 27733122.00 37148588.00 30466816.00 G 18011762.00 19257708.00 17593546.00 20012868.00 12050448.00 27073800.00 35066120.00 20892688.00 31068124.00 24686124.00 61556220.00 27359022.00 H 39437684.00 26569990.00 11628660.00 17160198.00 15952954.00 20839820.00 14141774.00 39349088.00 31200726.00 17204092.00 26353548.00 18747968.00