Load time: 08/23/2012 10:42:22 AM Read time: 08/23/2012 10:42:22 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2811 Comment: Plate 1 partial redo Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 113639768 cps Min counts: 489431 counts Well list: r0(a1:h9) = cps(f2(a1:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7468553.00 5941838.00 4387702.00 2359996.00 10150262.00 2386719.00 13512008.00 7044418.00 51057932.00 B 1123017.00 3725438.00 9316043.00 6503191.00 4269058.00 4345101.00 15480696.00 19273328.00 31469528.00 C 6089322.00 978862.00 3632707.00 3852394.00 8771774.00 5033628.00 15176828.00 113639768.00 18879322.00 D 3340964.00 2589807.00 4414461.00 6211817.00 4190345.00 3522855.00 10016393.00 46724308.00 11283147.00 E 3041573.00 3735784.00 3526730.00 4426409.00 2205784.00 4377971.00 4106352.00 51487092.00 6720800.00 F 4193002.00 6349056.00 3024153.00 6885451.00 2910188.00 11587810.00 25024828.00 16098227.00 3960772.00 G 2916244.00 1809178.00 3902449.00 4236554.00 2583818.00 4133780.00 12644538.00 26405696.00 2393869.00 H 2571057.00 5478586.00 7713558.00 5131089.00 7374241.00 5926116.00 16709572.00 36270648.00 1550456.00