Load time: 03/20/2013 11:14:34 AM Read time: 03/20/2013 11:14:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2983 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42487536 cps Min counts: 58013 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40934592.00 42487536.00 42319064.00 123300.00 B 24811382.00 24706572.00 24904256.00 121978.00 C 14718128.00 14996070.00 14753571.00 122564.00 D 7776062.00 8298356.00 8140673.00 125492.00 E 4998005.00 5195320.00 4720946.00 123905.00 F 2936885.00 2995706.00 2756298.00 123414.00 G 1801329.00 1871401.00 1727466.00 120297.00 H 1116620.00 1141141.00 1045383.00 116027.00 Load time: 03/20/2013 11:17:37 AM Read time: 03/20/2013 11:17:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2984 Comment: plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65510460 cps Min counts: 773770 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 40102732.00 46787128.00 19937188.00 34849804.00 28097864.00 17212258.00 39159204.00 28898656.00 30885824.00 22332254.00 29074240.00 33711512.00 B 41344264.00 65510460.00 23870894.00 23321926.00 25974860.00 28554302.00 33724440.00 20728006.00 23539602.00 25699566.00 23315180.00 22672860.00 C 40759500.00 51639760.00 22102732.00 23176904.00 30601946.00 32106116.00 30970132.00 26186624.00 29183128.00 23368976.00 28444612.00 38106436.00 D 45589912.00 62137740.00 29351506.00 38908540.00 29186872.00 31556958.00 46091224.00 15213728.00 25411842.00 34357412.00 26960542.00 25553838.00 E 62881564.00 51265472.00 41987700.00 27879292.00 29824746.00 38371184.00 45478976.00 20785750.00 32228472.00 23978750.00 26444822.00 27704802.00 F 58211916.00 32387518.00 20390976.00 30588518.00 34316576.00 46349088.00 55251152.00 22349478.00 32161228.00 19589516.00 27985062.00 34859380.00 G 60979128.00 29026034.00 18590682.00 30588640.00 29529156.00 40055608.00 60533560.00 21320742.00 31567768.00 20226856.00 34281156.00 36083296.00 H 39804840.00 48132308.00 29087904.00 39287648.00 23147992.00 30406532.00 41852116.00 15578660.00 29829688.00 23226232.00 18964074.00 33166318.00 Load time: 03/20/2013 11:32:03 AM Read time: 03/20/2013 11:32:03 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2985 Comment: standard redo Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 127566968 cps Min counts: 57768 counts Well list: r0(a1:h4,a5,b5,c5) = cps(f2(a1:h4,a5,b5,c5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36867444.00 37521588.00 37785996.00 120486.00 127566968.00 B 21919030.00 21734324.00 22251594.00 122790.00 123619288.00 C 13053200.00 13497366.00 13195443.00 121808.00 123080504.00 D 7152360.00 7537858.00 7273698.00 124698.00 E 4457936.00 4643325.00 4316298.00 121468.00 F 2601915.00 2692179.00 2447648.00 122072.00 G 1629222.00 1674663.00 1554052.00 118275.00 H 1011568.00 1034332.00 954480.00 115536.00 Load time: 03/20/2013 11:34:38 AM Read time: 03/20/2013 11:34:38 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2986 Comment: plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 38277976 cps Min counts: 66883 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 12599596.00 14457429.00 134295.00 29127762.00 2943484.00 5569829.00 4633723.00 1740076.00 2113948.00 882695.00 9809038.00 9679193.00 B 11533472.00 6025558.00 135183.00 17765156.00 2775814.00 5462434.00 6992359.00 8615127.00 13874086.00 2701949.00 16730889.00 10819706.00 C 14621785.00 7552219.00 137808.00 14233556.00 4940087.00 7910394.00 12583472.00 11635101.00 8106881.00 4415614.00 19089978.00 10475437.00 D 9415886.00 15111401.00 137714.00 19580992.00 4131331.00 7544870.00 4951159.00 5131177.00 10363934.00 1696634.00 13764677.00 6916604.00 E 12790647.00 16428136.00 141114.00 38277976.00 5720694.00 8351062.00 9099828.00 10523420.00 15634595.00 689857.00 21217902.00 6347217.00 F 5950192.00 24742594.00 133766.00 30874362.00 10316263.00 1824351.00 17738804.00 4133297.00 7392439.00 1010076.00 25883538.00 12638968.00 G 14391950.00 5199446.00 134238.00 10289867.00 4126253.00 3704050.00 2807578.00 3926659.00 18830328.00 871795.00 1873321.00 8348584.00 H 11005279.00 3544949.00 135806.00 9822149.00 9188033.00 11224033.00 1993302.00 2512975.00 1594986.00 724332.00 2711909.00 6660046.00 Load time: 03/20/2013 11:36:34 AM Read time: 03/20/2013 11:36:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2987 Comment: plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27442066 cps Min counts: 514426 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6027262.00 8459602.00 4981963.00 8180738.00 8924695.00 6328678.00 1970962.00 3489813.00 17136174.00 8083168.00 13511979.00 11946043.00 B 4771038.00 5620965.00 18208672.00 6219693.00 12612083.00 5684680.00 4311416.00 12734024.00 1883687.00 14132789.00 4098014.00 15675424.00 C 3956898.00 8085331.00 12347607.00 9207856.00 10963693.00 7064362.00 7538938.00 5723563.00 11212567.00 7257591.00 6519473.00 19349770.00 D 5702602.00 10679837.00 9496408.00 11700231.00 10476174.00 11388670.00 2197873.00 10479126.00 19794734.00 8402098.00 12766905.00 13601451.00 E 6213584.00 7199523.00 8685021.00 9551351.00 8922997.00 9961971.00 1028853.00 5258871.00 27442066.00 7592019.00 7245066.00 15065532.00 F 10327870.00 20966484.00 9451945.00 11397646.00 15078537.00 6704249.00 1261230.00 9616198.00 8531028.00 13328135.00 15544859.00 13628785.00 G 3079457.00 22197910.00 5809426.00 21837434.00 19672342.00 9706014.00 1148810.00 4304860.00 8272432.00 12115146.00 6742314.00 7482728.00 H 3890079.00 4022785.00 6600361.00 6980827.00 5865423.00 11139620.00 1177449.00 13376337.00 23401488.00 17452532.00 8835606.00 14099080.00